Strain Fitness in Escherichia coli ECRC100 around OKFHMN_13385

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiC and thiE overlap by 1 nucleotidesthiE and thiF overlap by 8 nucleotidesthiF and thiS overlap by 17 nucleotidesthiS and thiG are separated by 1 nucleotidesthiG and thiH overlap by 4 nucleotides OKFHMN_13375: thiC - phosphomethylpyrimidine synthase ThiC, at 2,570,118 to 2,572,013 thiC OKFHMN_13380: thiE - thiamine phosphate synthase, at 2,572,013 to 2,572,648 thiE OKFHMN_13385: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 2,572,641 to 2,573,396 thiF OKFHMN_13390: thiS - sulfur carrier protein ThiS, at 2,573,380 to 2,573,580 thiS OKFHMN_13395: thiG - thiazole synthase, at 2,573,582 to 2,574,352 thiG OKFHMN_13400: thiH - 2-iminoacetate synthase ThiH, at 2,574,349 to 2,575,482 thiH Position (kb) 2572 2573 2574Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2571.648 kb on - strand, within thiCat 2571.659 kb on + strand, within thiCat 2571.666 kb on + strand, within thiCat 2571.667 kb on - strand, within thiCat 2571.668 kb on + strand, within thiCat 2571.668 kb on + strand, within thiCat 2571.797 kb on + strand, within thiCat 2571.810 kb on + strand, within thiCat 2571.915 kb on + strandat 2571.932 kb on + strandat 2571.932 kb on + strandat 2571.946 kb on + strandat 2571.954 kb on - strandat 2571.991 kb on - strandat 2571.991 kb on - strandat 2571.999 kb on - strandat 2572.016 kb on + strandat 2572.016 kb on + strandat 2572.017 kb on - strandat 2572.017 kb on - strandat 2572.077 kb on + strand, within thiEat 2572.077 kb on + strand, within thiEat 2572.077 kb on + strand, within thiEat 2572.077 kb on + strand, within thiEat 2572.077 kb on + strand, within thiEat 2572.078 kb on - strand, within thiEat 2572.078 kb on - strand, within thiEat 2572.110 kb on + strand, within thiEat 2572.110 kb on + strand, within thiEat 2572.111 kb on - strand, within thiEat 2572.114 kb on + strand, within thiEat 2572.114 kb on + strand, within thiEat 2572.114 kb on + strand, within thiEat 2572.115 kb on - strand, within thiEat 2572.126 kb on - strand, within thiEat 2572.126 kb on - strand, within thiEat 2572.199 kb on - strand, within thiEat 2572.385 kb on + strand, within thiEat 2572.385 kb on + strand, within thiEat 2572.386 kb on - strand, within thiEat 2572.668 kb on - strandat 2572.760 kb on + strand, within thiFat 2572.774 kb on + strand, within thiFat 2572.776 kb on + strand, within thiFat 2572.777 kb on - strand, within thiFat 2572.835 kb on - strand, within thiFat 2572.875 kb on - strand, within thiFat 2572.948 kb on + strand, within thiFat 2572.948 kb on + strand, within thiFat 2572.948 kb on + strand, within thiFat 2572.948 kb on + strand, within thiFat 2572.949 kb on - strand, within thiFat 2572.949 kb on - strand, within thiFat 2572.949 kb on - strand, within thiFat 2572.976 kb on - strand, within thiFat 2573.024 kb on + strand, within thiFat 2573.045 kb on + strand, within thiFat 2573.045 kb on + strand, within thiFat 2573.258 kb on + strand, within thiFat 2573.259 kb on - strand, within thiFat 2573.259 kb on - strand, within thiFat 2573.264 kb on - strand, within thiFat 2573.312 kb on + strand, within thiFat 2573.488 kb on - strand, within thiSat 2573.526 kb on + strand, within thiSat 2573.526 kb on + strand, within thiSat 2573.526 kb on + strand, within thiSat 2573.527 kb on - strand, within thiSat 2573.587 kb on - strandat 2573.785 kb on + strand, within thiGat 2573.786 kb on - strand, within thiGat 2574.089 kb on - strand, within thiGat 2574.089 kb on - strand, within thiGat 2574.186 kb on + strand, within thiGat 2574.299 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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2,571,648 - thiC OKFHMN_13375 0.81 -0.6
2,571,659 + thiC OKFHMN_13375 0.81 -0.9
2,571,666 + thiC OKFHMN_13375 0.82 -2.2
2,571,667 - thiC OKFHMN_13375 0.82 -1.9
2,571,668 + thiC OKFHMN_13375 0.82 -0.2
2,571,668 + thiC OKFHMN_13375 0.82 -1.0
2,571,797 + thiC OKFHMN_13375 0.89 -1.1
2,571,810 + thiC OKFHMN_13375 0.89 -0.3
2,571,915 + -2.0
2,571,932 + +0.4
2,571,932 + -0.8
2,571,946 + -0.9
2,571,954 - -2.8
2,571,991 - +0.3
2,571,991 - -1.0
2,571,999 - -2.2
2,572,016 + -1.0
2,572,016 + -1.0
2,572,017 - -1.0
2,572,017 - -0.7
2,572,077 + thiE OKFHMN_13380 0.10 -2.4
2,572,077 + thiE OKFHMN_13380 0.10 -1.0
2,572,077 + thiE OKFHMN_13380 0.10 -0.5
2,572,077 + thiE OKFHMN_13380 0.10 -1.0
2,572,077 + thiE OKFHMN_13380 0.10 +0.1
2,572,078 - thiE OKFHMN_13380 0.10 -1.4
2,572,078 - thiE OKFHMN_13380 0.10 +0.3
2,572,110 + thiE OKFHMN_13380 0.15 -1.8
2,572,110 + thiE OKFHMN_13380 0.15 +0.8
2,572,111 - thiE OKFHMN_13380 0.15 -1.1
2,572,114 + thiE OKFHMN_13380 0.16 -1.3
2,572,114 + thiE OKFHMN_13380 0.16 -2.9
2,572,114 + thiE OKFHMN_13380 0.16 -1.2
2,572,115 - thiE OKFHMN_13380 0.16 -1.4
2,572,126 - thiE OKFHMN_13380 0.18 -1.9
2,572,126 - thiE OKFHMN_13380 0.18 -1.3
2,572,199 - thiE OKFHMN_13380 0.29 -2.2
2,572,385 + thiE OKFHMN_13380 0.58 -1.3
2,572,385 + thiE OKFHMN_13380 0.58 -0.5
2,572,386 - thiE OKFHMN_13380 0.59 -1.9
2,572,668 - -1.9
2,572,760 + thiF OKFHMN_13385 0.16 -0.9
2,572,774 + thiF OKFHMN_13385 0.18 -1.3
2,572,776 + thiF OKFHMN_13385 0.18 -1.1
2,572,777 - thiF OKFHMN_13385 0.18 -0.2
2,572,835 - thiF OKFHMN_13385 0.26 -0.5
2,572,875 - thiF OKFHMN_13385 0.31 -2.4
2,572,948 + thiF OKFHMN_13385 0.41 -3.2
2,572,948 + thiF OKFHMN_13385 0.41 -1.8
2,572,948 + thiF OKFHMN_13385 0.41 -0.7
2,572,948 + thiF OKFHMN_13385 0.41 -0.6
2,572,949 - thiF OKFHMN_13385 0.41 +0.3
2,572,949 - thiF OKFHMN_13385 0.41 -1.7
2,572,949 - thiF OKFHMN_13385 0.41 -0.6
2,572,976 - thiF OKFHMN_13385 0.44 -0.5
2,573,024 + thiF OKFHMN_13385 0.51 -2.1
2,573,045 + thiF OKFHMN_13385 0.53 -1.5
2,573,045 + thiF OKFHMN_13385 0.53 -0.8
2,573,258 + thiF OKFHMN_13385 0.82 -2.2
2,573,259 - thiF OKFHMN_13385 0.82 -1.4
2,573,259 - thiF OKFHMN_13385 0.82 -1.1
2,573,264 - thiF OKFHMN_13385 0.82 +1.8
2,573,312 + thiF OKFHMN_13385 0.89 -1.0
2,573,488 - thiS OKFHMN_13390 0.54 -0.5
2,573,526 + thiS OKFHMN_13390 0.73 -0.3
2,573,526 + thiS OKFHMN_13390 0.73 -2.7
2,573,526 + thiS OKFHMN_13390 0.73 -1.5
2,573,527 - thiS OKFHMN_13390 0.73 -0.6
2,573,587 - -0.5
2,573,785 + thiG OKFHMN_13395 0.26 -1.9
2,573,786 - thiG OKFHMN_13395 0.26 +0.0
2,574,089 - thiG OKFHMN_13395 0.66 -0.7
2,574,089 - thiG OKFHMN_13395 0.66 -3.7
2,574,186 + thiG OKFHMN_13395 0.78 -0.4
2,574,299 + -0.7

Or see this region's nucleotide sequence