Strain Fitness in Escherichia coli ECRC100 around OKFHMN_12270

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmutL and amiB are separated by 9 nucleotidesamiB and tsaE are separated by 18 nucleotidestsaE and nnr overlap by 29 nucleotides OKFHMN_12265: mutL - DNA mismatch repair endonuclease MutL, at 2,348,542 to 2,350,389 mutL OKFHMN_12270: amiB - N-acetylmuramoyl-L-alanine amidase AmiB, at 2,350,399 to 2,351,736 amiB OKFHMN_12275: tsaE - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 2,351,755 to 2,352,216 tsaE OKFHMN_12280: nnr - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 2,352,188 to 2,353,720 nnr Position (kb) 2350 2351 2352Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2349.401 kb on - strand, within mutLat 2349.427 kb on - strand, within mutLat 2349.427 kb on - strand, within mutLat 2349.518 kb on + strand, within mutLat 2349.518 kb on + strand, within mutLat 2349.519 kb on - strand, within mutLat 2349.620 kb on + strand, within mutLat 2349.886 kb on - strand, within mutLat 2349.886 kb on - strand, within mutLat 2349.886 kb on - strand, within mutLat 2349.935 kb on + strand, within mutLat 2349.949 kb on + strand, within mutLat 2350.087 kb on - strand, within mutLat 2350.155 kb on + strand, within mutLat 2350.156 kb on - strand, within mutLat 2350.230 kb on + strandat 2350.231 kb on - strandat 2350.372 kb on + strandat 2350.372 kb on + strandat 2350.372 kb on + strandat 2350.434 kb on + strandat 2350.493 kb on + strandat 2350.494 kb on - strandat 2350.539 kb on - strand, within amiBat 2350.582 kb on - strand, within amiBat 2350.601 kb on - strand, within amiBat 2350.659 kb on + strand, within amiBat 2350.659 kb on + strand, within amiBat 2350.659 kb on + strand, within amiBat 2350.660 kb on - strand, within amiBat 2350.660 kb on - strand, within amiBat 2350.660 kb on - strand, within amiBat 2350.663 kb on + strand, within amiBat 2350.695 kb on + strand, within amiBat 2350.696 kb on - strand, within amiBat 2350.705 kb on + strand, within amiBat 2350.705 kb on + strand, within amiBat 2350.706 kb on - strand, within amiBat 2350.706 kb on - strand, within amiBat 2350.706 kb on - strand, within amiBat 2350.712 kb on + strand, within amiBat 2350.712 kb on + strand, within amiBat 2350.712 kb on + strand, within amiBat 2350.712 kb on + strand, within amiBat 2350.713 kb on - strand, within amiBat 2350.762 kb on + strand, within amiBat 2350.762 kb on + strand, within amiBat 2350.762 kb on + strand, within amiBat 2350.763 kb on - strand, within amiBat 2350.995 kb on + strand, within amiBat 2351.000 kb on + strand, within amiBat 2351.029 kb on - strand, within amiBat 2351.056 kb on - strand, within amiBat 2351.211 kb on + strand, within amiBat 2351.212 kb on - strand, within amiBat 2351.212 kb on - strand, within amiBat 2351.299 kb on - strand, within amiBat 2351.381 kb on + strand, within amiBat 2351.381 kb on + strand, within amiBat 2351.382 kb on - strand, within amiBat 2351.382 kb on - strand, within amiBat 2351.467 kb on - strand, within amiBat 2351.500 kb on - strand, within amiBat 2351.500 kb on - strand, within amiBat 2351.607 kb on + strandat 2351.643 kb on + strandat 2351.729 kb on + strandat 2351.729 kb on + strandat 2351.729 kb on + strandat 2351.729 kb on + strandat 2351.729 kb on + strandat 2351.730 kb on - strandat 2352.242 kb on - strandat 2352.248 kb on - strandat 2352.395 kb on - strand, within nnrat 2352.395 kb on - strand, within nnrat 2352.531 kb on - strand, within nnrat 2352.531 kb on - strand, within nnrat 2352.531 kb on - strand, within nnrat 2352.551 kb on + strand, within nnrat 2352.587 kb on + strand, within nnrat 2352.588 kb on - strand, within nnr

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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2,349,401 - mutL OKFHMN_12265 0.46 -1.2
2,349,427 - mutL OKFHMN_12265 0.48 +0.1
2,349,427 - mutL OKFHMN_12265 0.48 +1.6
2,349,518 + mutL OKFHMN_12265 0.53 +0.5
2,349,518 + mutL OKFHMN_12265 0.53 -0.3
2,349,519 - mutL OKFHMN_12265 0.53 +1.2
2,349,620 + mutL OKFHMN_12265 0.58 +0.5
2,349,886 - mutL OKFHMN_12265 0.73 +0.0
2,349,886 - mutL OKFHMN_12265 0.73 +0.2
2,349,886 - mutL OKFHMN_12265 0.73 -3.2
2,349,935 + mutL OKFHMN_12265 0.75 +0.1
2,349,949 + mutL OKFHMN_12265 0.76 +0.5
2,350,087 - mutL OKFHMN_12265 0.84 +0.6
2,350,155 + mutL OKFHMN_12265 0.87 +0.8
2,350,156 - mutL OKFHMN_12265 0.87 +1.1
2,350,230 + -0.8
2,350,231 - +0.3
2,350,372 + -0.1
2,350,372 + +1.2
2,350,372 + +0.6
2,350,434 + -0.6
2,350,493 + +0.1
2,350,494 - +0.5
2,350,539 - amiB OKFHMN_12270 0.10 -0.2
2,350,582 - amiB OKFHMN_12270 0.14 +0.7
2,350,601 - amiB OKFHMN_12270 0.15 -0.3
2,350,659 + amiB OKFHMN_12270 0.19 +0.1
2,350,659 + amiB OKFHMN_12270 0.19 +0.9
2,350,659 + amiB OKFHMN_12270 0.19 -0.4
2,350,660 - amiB OKFHMN_12270 0.20 +1.2
2,350,660 - amiB OKFHMN_12270 0.20 -0.0
2,350,660 - amiB OKFHMN_12270 0.20 +0.7
2,350,663 + amiB OKFHMN_12270 0.20 +0.4
2,350,695 + amiB OKFHMN_12270 0.22 -0.6
2,350,696 - amiB OKFHMN_12270 0.22 -0.2
2,350,705 + amiB OKFHMN_12270 0.23 +0.1
2,350,705 + amiB OKFHMN_12270 0.23 +2.5
2,350,706 - amiB OKFHMN_12270 0.23 +0.8
2,350,706 - amiB OKFHMN_12270 0.23 -0.3
2,350,706 - amiB OKFHMN_12270 0.23 +0.0
2,350,712 + amiB OKFHMN_12270 0.23 -0.4
2,350,712 + amiB OKFHMN_12270 0.23 +0.3
2,350,712 + amiB OKFHMN_12270 0.23 +0.4
2,350,712 + amiB OKFHMN_12270 0.23 +0.3
2,350,713 - amiB OKFHMN_12270 0.23 +0.6
2,350,762 + amiB OKFHMN_12270 0.27 -0.5
2,350,762 + amiB OKFHMN_12270 0.27 +1.0
2,350,762 + amiB OKFHMN_12270 0.27 -0.2
2,350,763 - amiB OKFHMN_12270 0.27 +0.4
2,350,995 + amiB OKFHMN_12270 0.45 +0.1
2,351,000 + amiB OKFHMN_12270 0.45 -0.7
2,351,029 - amiB OKFHMN_12270 0.47 -0.9
2,351,056 - amiB OKFHMN_12270 0.49 +0.3
2,351,211 + amiB OKFHMN_12270 0.61 +0.8
2,351,212 - amiB OKFHMN_12270 0.61 +0.3
2,351,212 - amiB OKFHMN_12270 0.61 -0.3
2,351,299 - amiB OKFHMN_12270 0.67 +0.0
2,351,381 + amiB OKFHMN_12270 0.73 -0.2
2,351,381 + amiB OKFHMN_12270 0.73 -0.4
2,351,382 - amiB OKFHMN_12270 0.73 -0.2
2,351,382 - amiB OKFHMN_12270 0.73 +0.4
2,351,467 - amiB OKFHMN_12270 0.80 -0.6
2,351,500 - amiB OKFHMN_12270 0.82 -1.1
2,351,500 - amiB OKFHMN_12270 0.82 +0.8
2,351,607 + +0.5
2,351,643 + +0.5
2,351,729 + +0.6
2,351,729 + -0.4
2,351,729 + -3.2
2,351,729 + -0.0
2,351,729 + +0.1
2,351,730 - +0.1
2,352,242 - +1.0
2,352,248 - +0.7
2,352,395 - nnr OKFHMN_12280 0.14 +0.1
2,352,395 - nnr OKFHMN_12280 0.14 -1.2
2,352,531 - nnr OKFHMN_12280 0.22 +0.9
2,352,531 - nnr OKFHMN_12280 0.22 +0.2
2,352,531 - nnr OKFHMN_12280 0.22 +0.1
2,352,551 + nnr OKFHMN_12280 0.24 -2.9
2,352,587 + nnr OKFHMN_12280 0.26 +0.4
2,352,588 - nnr OKFHMN_12280 0.26 +0.4

Or see this region's nucleotide sequence