Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ulaF and ulaE overlap by 1 nucleotides ulaE and ulaD are separated by 9 nucleotides ulaD and ulaC are separated by 13 nucleotides ulaC and ulaB are separated by 9 nucleotides ulaB and ulaA are separated by 15 nucleotides
OKFHMN_12125: ulaF - L-ribulose-5-phosphate 4-epimerase UlaF, at 2,323,413 to 2,324,099
ulaF
OKFHMN_12130: ulaE - L-ribulose-5-phosphate 3-epimerase UlaE, at 2,324,099 to 2,324,953
ulaE
OKFHMN_12135: ulaD - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD, at 2,324,963 to 2,325,613
ulaD
OKFHMN_12140: ulaC - PTS ascorbate transporter subunit IIA, at 2,325,627 to 2,326,091
ulaC
OKFHMN_12145: ulaB - PTS ascorbate transporter subunit IIB, at 2,326,101 to 2,326,406
ulaB
OKFHMN_12150: ulaA - PTS ascorbate transporter subunit IIC, at 2,326,422 to 2,327,819
ulaA
Position (kb)
2324
2325
2326 Strain fitness (log2 ratio)
-1
0
1 at 2324.010 kb on - strand, within ulaF at 2324.100 kb on + strand at 2324.211 kb on + strand, within ulaE at 2324.324 kb on + strand, within ulaE at 2324.325 kb on - strand, within ulaE at 2324.433 kb on + strand, within ulaE at 2324.586 kb on + strand, within ulaE at 2324.589 kb on + strand, within ulaE at 2324.654 kb on - strand, within ulaE at 2324.660 kb on - strand, within ulaE at 2324.835 kb on - strand, within ulaE at 2324.954 kb on - strand at 2325.392 kb on - strand, within ulaD at 2325.392 kb on - strand, within ulaD at 2325.663 kb on + strand at 2325.922 kb on + strand, within ulaC at 2325.961 kb on + strand, within ulaC at 2325.961 kb on + strand, within ulaC at 2325.962 kb on - strand, within ulaC at 2326.084 kb on - strand at 2326.102 kb on + strand at 2326.103 kb on - strand at 2326.195 kb on + strand, within ulaB at 2326.195 kb on + strand, within ulaB at 2326.235 kb on + strand, within ulaB at 2326.305 kb on - strand, within ulaB at 2326.305 kb on - strand, within ulaB at 2326.398 kb on + strand at 2326.407 kb on - strand at 2326.424 kb on - strand at 2326.493 kb on + strand at 2326.494 kb on - strand at 2326.494 kb on - strand at 2326.494 kb on - strand at 2326.494 kb on - strand at 2326.495 kb on + strand at 2326.495 kb on + strand at 2326.495 kb on + strand at 2326.495 kb on + strand at 2326.495 kb on + strand at 2326.495 kb on + strand at 2326.496 kb on - strand at 2326.496 kb on - strand at 2326.496 kb on - strand at 2326.496 kb on - strand at 2326.610 kb on + strand, within ulaA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 2,324,010 - ulaF OKFHMN_12125 0.87 +1.0 2,324,100 + +0.1 2,324,211 + ulaE OKFHMN_12130 0.13 +0.6 2,324,324 + ulaE OKFHMN_12130 0.26 -0.3 2,324,325 - ulaE OKFHMN_12130 0.26 +0.4 2,324,433 + ulaE OKFHMN_12130 0.39 -0.9 2,324,586 + ulaE OKFHMN_12130 0.57 -0.7 2,324,589 + ulaE OKFHMN_12130 0.57 -0.3 2,324,654 - ulaE OKFHMN_12130 0.65 -0.7 2,324,660 - ulaE OKFHMN_12130 0.66 -0.7 2,324,835 - ulaE OKFHMN_12130 0.86 -0.2 2,324,954 - -0.1 2,325,392 - ulaD OKFHMN_12135 0.66 +0.1 2,325,392 - ulaD OKFHMN_12135 0.66 +0.2 2,325,663 + +0.4 2,325,922 + ulaC OKFHMN_12140 0.63 -0.1 2,325,961 + ulaC OKFHMN_12140 0.72 +0.3 2,325,961 + ulaC OKFHMN_12140 0.72 +0.3 2,325,962 - ulaC OKFHMN_12140 0.72 -0.6 2,326,084 - +0.7 2,326,102 + -1.1 2,326,103 - -0.5 2,326,195 + ulaB OKFHMN_12145 0.31 -0.1 2,326,195 + ulaB OKFHMN_12145 0.31 -1.1 2,326,235 + ulaB OKFHMN_12145 0.44 +0.9 2,326,305 - ulaB OKFHMN_12145 0.67 -0.8 2,326,305 - ulaB OKFHMN_12145 0.67 -0.4 2,326,398 + -0.2 2,326,407 - +0.2 2,326,424 - +0.2 2,326,493 + -0.0 2,326,494 - -0.2 2,326,494 - +0.3 2,326,494 - +0.8 2,326,494 - -0.2 2,326,495 + -0.2 2,326,495 + -0.8 2,326,495 + -0.5 2,326,495 + +1.0 2,326,495 + +0.7 2,326,495 + +0.1 2,326,496 - -1.7 2,326,496 - +0.2 2,326,496 - +0.5 2,326,496 - +0.2 2,326,610 + ulaA OKFHMN_12150 0.13 +0.0
Or see this region's nucleotide sequence