Strain Fitness in Escherichia coli ECRC100 around OKFHMN_10850

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntapaG and apaH are separated by 6 nucleotidesapaH and OKFHMN_10850 overlap by 4 nucleotidesOKFHMN_10850 and OKFHMN_10855 are separated by 2 nucleotidesOKFHMN_10855 and OKFHMN_10860 are separated by 85 nucleotidesOKFHMN_10860 and kefC are separated by 80 nucleotides OKFHMN_10840: apaG - Co2+/Mg2+ efflux protein ApaG, at 2,046,595 to 2,046,972 apaG OKFHMN_10845: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 2,046,979 to 2,047,827 apaH OKFHMN_10850: OKFHMN_10850 - plasmid maintenance protein CcdB, at 2,047,824 to 2,048,138 _10850 OKFHMN_10855: OKFHMN_10855 - antitoxin, at 2,048,141 to 2,048,374 _10855 OKFHMN_10860: OKFHMN_10860 - Dihydrofolate reductase, at 2,048,460 to 2,049,050 _10860 OKFHMN_10865: kefC - glutathione-regulated potassium-efflux system protein KefC, at 2,049,131 to 2,050,993 kefC Position (kb) 2047 2048 2049Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2046.838 kb on + strand, within apaGat 2046.988 kb on + strandat 2046.989 kb on - strandat 2047.154 kb on + strand, within apaHat 2047.154 kb on + strand, within apaHat 2047.155 kb on - strand, within apaHat 2047.275 kb on + strand, within apaHat 2047.275 kb on + strand, within apaHat 2047.276 kb on - strand, within apaHat 2047.395 kb on - strand, within apaHat 2047.440 kb on + strand, within apaHat 2047.558 kb on - strand, within apaHat 2047.561 kb on + strand, within apaHat 2047.562 kb on - strand, within apaHat 2047.562 kb on - strand, within apaHat 2047.736 kb on + strand, within apaHat 2047.765 kb on + strandat 2047.859 kb on + strand, within OKFHMN_10850at 2047.860 kb on - strand, within OKFHMN_10850at 2047.949 kb on - strand, within OKFHMN_10850at 2047.963 kb on - strand, within OKFHMN_10850at 2047.963 kb on - strand, within OKFHMN_10850at 2048.021 kb on + strand, within OKFHMN_10850at 2048.022 kb on - strand, within OKFHMN_10850at 2048.029 kb on - strand, within OKFHMN_10850at 2048.037 kb on - strand, within OKFHMN_10850at 2048.052 kb on - strand, within OKFHMN_10850at 2048.063 kb on + strand, within OKFHMN_10850at 2048.122 kb on + strandat 2048.123 kb on - strandat 2048.123 kb on - strandat 2048.142 kb on + strandat 2048.206 kb on + strand, within OKFHMN_10855at 2048.276 kb on - strand, within OKFHMN_10855at 2048.290 kb on + strand, within OKFHMN_10855at 2048.294 kb on + strand, within OKFHMN_10855at 2048.295 kb on - strand, within OKFHMN_10855at 2048.295 kb on - strand, within OKFHMN_10855at 2048.399 kb on + strandat 2048.400 kb on - strandat 2048.400 kb on - strandat 2048.428 kb on + strandat 2048.429 kb on - strandat 2048.452 kb on - strandat 2048.453 kb on + strandat 2048.454 kb on - strandat 2048.454 kb on - strandat 2048.978 kb on + strand, within OKFHMN_10860at 2048.979 kb on - strand, within OKFHMN_10860at 2049.076 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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2,046,838 + apaG OKFHMN_10840 0.64 -0.6
2,046,988 + +0.3
2,046,989 - -0.3
2,047,154 + apaH OKFHMN_10845 0.21 -0.3
2,047,154 + apaH OKFHMN_10845 0.21 -1.3
2,047,155 - apaH OKFHMN_10845 0.21 -2.2
2,047,275 + apaH OKFHMN_10845 0.35 -2.6
2,047,275 + apaH OKFHMN_10845 0.35 +0.7
2,047,276 - apaH OKFHMN_10845 0.35 -2.9
2,047,395 - apaH OKFHMN_10845 0.49 -1.1
2,047,440 + apaH OKFHMN_10845 0.54 -1.4
2,047,558 - apaH OKFHMN_10845 0.68 -1.5
2,047,561 + apaH OKFHMN_10845 0.69 -1.0
2,047,562 - apaH OKFHMN_10845 0.69 +1.4
2,047,562 - apaH OKFHMN_10845 0.69 -0.2
2,047,736 + apaH OKFHMN_10845 0.89 -0.4
2,047,765 + -0.4
2,047,859 + OKFHMN_10850 0.11 +0.5
2,047,860 - OKFHMN_10850 0.11 -0.4
2,047,949 - OKFHMN_10850 0.40 -1.1
2,047,963 - OKFHMN_10850 0.44 +2.0
2,047,963 - OKFHMN_10850 0.44 -0.5
2,048,021 + OKFHMN_10850 0.63 +1.2
2,048,022 - OKFHMN_10850 0.63 +0.1
2,048,029 - OKFHMN_10850 0.65 +0.4
2,048,037 - OKFHMN_10850 0.68 -0.6
2,048,052 - OKFHMN_10850 0.72 +0.9
2,048,063 + OKFHMN_10850 0.76 +0.3
2,048,122 + -1.1
2,048,123 - -0.2
2,048,123 - +0.2
2,048,142 + -0.9
2,048,206 + OKFHMN_10855 0.28 +0.7
2,048,276 - OKFHMN_10855 0.58 -0.4
2,048,290 + OKFHMN_10855 0.64 -0.0
2,048,294 + OKFHMN_10855 0.65 -2.3
2,048,295 - OKFHMN_10855 0.66 +1.2
2,048,295 - OKFHMN_10855 0.66 -0.1
2,048,399 + +0.1
2,048,400 - -0.2
2,048,400 - +0.2
2,048,428 + -0.9
2,048,429 - -0.1
2,048,452 - -0.2
2,048,453 + +1.3
2,048,454 - -0.3
2,048,454 - -0.6
2,048,978 + OKFHMN_10860 0.88 -2.0
2,048,979 - OKFHMN_10860 0.88 +0.1
2,049,076 - +0.2

Or see this region's nucleotide sequence