Strain Fitness in Escherichia coli ECRC100 around OKFHMN_05210

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyccT and mgsA are separated by 95 nucleotidesmgsA and helD are separated by 31 nucleotideshelD and yccF are separated by 122 nucleotidesyccF and yccS are separated by 9 nucleotides OKFHMN_05200: yccT - DUF2057 domain-containing protein, at 858,924 to 859,586 yccT OKFHMN_05205: mgsA - methylglyoxal synthase, at 859,682 to 860,140 mgsA OKFHMN_05210: helD - DNA helicase IV, at 860,172 to 862,226 helD OKFHMN_05215: yccF - YccF domain-containing protein, at 862,349 to 862,795 yccF OKFHMN_05220: yccS - Inner membrane protein YccS, at 862,805 to 864,967 yccS Position (kb) 860 861 862 863Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 859.353 kb on + strand, within yccTat 859.354 kb on - strand, within yccTat 859.375 kb on - strand, within yccTat 859.548 kb on - strandat 859.635 kb on + strandat 859.644 kb on - strandat 859.648 kb on + strandat 859.649 kb on - strandat 859.656 kb on - strandat 859.676 kb on + strandat 859.721 kb on - strandat 859.788 kb on + strand, within mgsAat 859.803 kb on + strand, within mgsAat 859.833 kb on + strand, within mgsAat 859.959 kb on + strand, within mgsAat 860.288 kb on - strandat 860.714 kb on + strand, within helDat 860.715 kb on - strand, within helDat 861.306 kb on - strand, within helDat 861.384 kb on - strand, within helDat 861.429 kb on - strand, within helDat 861.698 kb on - strand, within helDat 862.178 kb on - strandat 862.448 kb on - strand, within yccFat 862.517 kb on - strand, within yccFat 862.517 kb on - strand, within yccFat 862.801 kb on + strandat 862.818 kb on + strandat 862.838 kb on + strandat 862.839 kb on - strandat 862.927 kb on - strandat 863.186 kb on + strand, within yccSat 863.186 kb on + strand, within yccSat 863.187 kb on - strand, within yccSat 863.211 kb on - strand, within yccS

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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859,353 + yccT OKFHMN_05200 0.65 -0.2
859,354 - yccT OKFHMN_05200 0.65 +0.5
859,375 - yccT OKFHMN_05200 0.68 -0.6
859,548 - -2.9
859,635 + -0.2
859,644 - +0.7
859,648 + -0.1
859,649 - +1.7
859,656 - -0.1
859,676 + -1.7
859,721 - -1.9
859,788 + mgsA OKFHMN_05205 0.23 -0.6
859,803 + mgsA OKFHMN_05205 0.26 +1.2
859,833 + mgsA OKFHMN_05205 0.33 -0.4
859,959 + mgsA OKFHMN_05205 0.60 +0.4
860,288 - -0.7
860,714 + helD OKFHMN_05210 0.26 +0.4
860,715 - helD OKFHMN_05210 0.26 +0.7
861,306 - helD OKFHMN_05210 0.55 -0.2
861,384 - helD OKFHMN_05210 0.59 +0.2
861,429 - helD OKFHMN_05210 0.61 +0.2
861,698 - helD OKFHMN_05210 0.74 +0.0
862,178 - -1.6
862,448 - yccF OKFHMN_05215 0.22 +0.2
862,517 - yccF OKFHMN_05215 0.38 +0.7
862,517 - yccF OKFHMN_05215 0.38 -1.9
862,801 + +1.1
862,818 + +0.8
862,838 + +0.1
862,839 - -0.8
862,927 - -4.7
863,186 + yccS OKFHMN_05220 0.18 +0.2
863,186 + yccS OKFHMN_05220 0.18 -1.0
863,187 - yccS OKFHMN_05220 0.18 +0.6
863,211 - yccS OKFHMN_05220 0.19 -0.3

Or see this region's nucleotide sequence