Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rutE and rutF are separated by 10 nucleotides rutF and rutG are separated by 20 nucleotides rutG and OKFHMN_04650 are separated by 82 nucleotides OKFHMN_04650 and wrbA are separated by 371 nucleotides
OKFHMN_04635: rutE - malonic semialdehyde reductase, at 766,959 to 767,549
rutE
OKFHMN_04640: rutF - pyrimidine utilization flavin reductase protein F, at 767,560 to 768,054
rutF
OKFHMN_04645: rutG - pyrimidine utilization transport protein G, at 768,075 to 769,403
rutG
OKFHMN_04650: OKFHMN_04650 - Stress-induced protein, KGG, repeat, at 769,486 to 769,593
_04650
OKFHMN_04655: wrbA - NAD(P)H:quinone oxidoreductase, at 769,965 to 770,561
wrbA
Position (kb) 768
769
770 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 767.214 kb on - strand, within rutE at 767.419 kb on + strand, within rutE at 767.560 kb on - strand at 767.771 kb on + strand, within rutF at 767.772 kb on - strand, within rutF at 767.775 kb on + strand, within rutF at 768.220 kb on + strand, within rutG at 768.221 kb on - strand, within rutG at 768.322 kb on - strand, within rutG at 768.865 kb on + strand, within rutG at 768.915 kb on + strand, within rutG at 768.915 kb on + strand, within rutG at 768.993 kb on + strand, within rutG at 769.005 kb on - strand, within rutG at 769.345 kb on + strand at 769.401 kb on + strand at 769.478 kb on + strand at 769.615 kb on - strand at 769.624 kb on - strand at 769.624 kb on - strand at 769.679 kb on - strand at 769.720 kb on - strand at 769.784 kb on - strand at 769.865 kb on + strand at 769.904 kb on - strand at 769.944 kb on + strand at 770.228 kb on + strand, within wrbA at 770.228 kb on + strand, within wrbA at 770.272 kb on + strand, within wrbA at 770.297 kb on + strand, within wrbA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 767,214 - rutE OKFHMN_04635 0.43 -0.5 767,419 + rutE OKFHMN_04635 0.78 +1.8 767,560 - +0.4 767,771 + rutF OKFHMN_04640 0.43 +0.8 767,772 - rutF OKFHMN_04640 0.43 -0.3 767,775 + rutF OKFHMN_04640 0.43 -0.5 768,220 + rutG OKFHMN_04645 0.11 -2.7 768,221 - rutG OKFHMN_04645 0.11 -0.3 768,322 - rutG OKFHMN_04645 0.19 +0.5 768,865 + rutG OKFHMN_04645 0.59 -1.4 768,915 + rutG OKFHMN_04645 0.63 -0.7 768,915 + rutG OKFHMN_04645 0.63 +1.8 768,993 + rutG OKFHMN_04645 0.69 +0.2 769,005 - rutG OKFHMN_04645 0.70 +0.3 769,345 + -1.0 769,401 + +0.5 769,478 + -3.2 769,615 - +0.9 769,624 - -1.7 769,624 - -0.2 769,679 - +0.4 769,720 - +0.4 769,784 - +0.9 769,865 + -0.4 769,904 - -1.4 769,944 + -1.2 770,228 + wrbA OKFHMN_04655 0.44 -0.4 770,228 + wrbA OKFHMN_04655 0.44 -1.2 770,272 + wrbA OKFHMN_04655 0.51 +1.1 770,297 + wrbA OKFHMN_04655 0.56 +0.5
Or see this region's nucleotide sequence