Strain Fitness in Escherichia coli ECRC100 around OKFHMN_04535

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nteAL and dgcT are separated by 97 nucleotidesdgcT and pgaA are separated by 586 nucleotides OKFHMN_04530: eAL - EAL domain, c-di-GMP-specific phosphodiesterase class I (or its enzymatically inactive variant), at 739,841 to 741,451 eAL OKFHMN_04535: dgcT - putative diguanylate cyclase DgcT, at 741,549 to 742,907 dgcT OKFHMN_04540: pgaA - poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA, at 743,494 to 745,917 pgaA Position (kb) 741 742 743Strain fitness (log2 ratio) -2 -1 0 1at 741.693 kb on - strand, within dgcTat 741.722 kb on - strand, within dgcTat 741.862 kb on - strand, within dgcTat 742.320 kb on - strand, within dgcTat 742.870 kb on - strandat 742.926 kb on - strandat 742.933 kb on - strandat 743.052 kb on - strandat 743.815 kb on + strand, within pgaAat 743.848 kb on - strand, within pgaA

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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741,693 - dgcT OKFHMN_04535 0.11 -0.9
741,722 - dgcT OKFHMN_04535 0.13 +0.5
741,862 - dgcT OKFHMN_04535 0.23 -0.6
742,320 - dgcT OKFHMN_04535 0.57 -1.7
742,870 - +0.4
742,926 - +0.6
742,933 - -0.1
743,052 - -2.3
743,815 + pgaA OKFHMN_04540 0.13 -0.1
743,848 - pgaA OKFHMN_04540 0.15 +0.2

Or see this region's nucleotide sequence