Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysB and OKFHMN_02605 are separated by 209 nucleotides OKFHMN_02605 and yciN are separated by 379 nucleotides yciN and sohB are separated by 35 nucleotides
OKFHMN_02600: cysB - HTH-type transcriptional regulator CysB, at 409,379 to 410,353
cysB
OKFHMN_02605: OKFHMN_02605 - type I DNA topoisomerase, at 410,563 to 413,160
_02605
OKFHMN_02610: yciN - Protein YciN, at 413,540 to 413,791
yciN
OKFHMN_02615: sohB - protease SohB, at 413,827 to 414,876
sohB
Position (kb) 410
411
412
413
414 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 410.428 kb on + strand at 410.473 kb on + strand at 410.479 kb on - strand at 410.479 kb on - strand at 410.480 kb on + strand at 410.480 kb on + strand at 410.481 kb on - strand at 410.500 kb on + strand at 410.686 kb on - strand at 413.209 kb on - strand at 413.334 kb on - strand at 413.334 kb on - strand at 413.401 kb on - strand at 413.481 kb on + strand at 413.514 kb on + strand at 413.514 kb on + strand at 413.617 kb on + strand, within yciN at 413.738 kb on - strand, within yciN at 413.774 kb on + strand at 413.864 kb on - strand at 413.931 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 410,428 + -2.7 410,473 + -0.2 410,479 - -0.5 410,479 - -0.0 410,480 + -0.1 410,480 + -0.4 410,481 - -0.7 410,500 + -0.5 410,686 - -2.0 413,209 - -0.0 413,334 - +0.6 413,334 - +1.4 413,401 - +1.1 413,481 + -0.9 413,514 + -3.6 413,514 + -1.0 413,617 + yciN OKFHMN_02610 0.31 -0.6 413,738 - yciN OKFHMN_02610 0.79 +0.2 413,774 + -0.1 413,864 - +2.1 413,931 + +0.6
Or see this region's nucleotide sequence