Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01550

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmddA and OKFHMN_01540 overlap by 4 nucleotidesOKFHMN_01540 and ydcY are separated by 0 nucleotidesydcY and ortT are separated by 85 nucleotidesortT and patD are separated by 386 nucleotides OKFHMN_01535: mddA - L-methionine sulfoximine/L-methionine sulfone acetyltransferase, at 216,457 to 216,975 mddA OKFHMN_01540: OKFHMN_01540 - Putative inner membrane exporter, YdcZ, at 216,972 to 217,421 _01540 OKFHMN_01545: ydcY - Uncharacterized protein YdcY, at 217,422 to 217,655 ydcY OKFHMN_01550: ortT - orphan toxin OrtT, at 217,741 to 217,914 ortT OKFHMN_01555: patD - aminobutyraldehyde dehydrogenase, at 218,301 to 219,725 patD Position (kb) 217 218Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 216.750 kb on + strand, within mddAat 217.068 kb on - strand, within OKFHMN_01540at 217.194 kb on - strand, within OKFHMN_01540at 217.420 kb on - strandat 218.140 kb on + strandat 218.174 kb on + strandat 218.360 kb on + strandat 218.361 kb on - strandat 218.651 kb on - strand, within patDat 218.651 kb on - strand, within patDat 218.653 kb on + strand, within patD

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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216,750 + mddA OKFHMN_01535 0.56 -3.9
217,068 - OKFHMN_01540 0.21 -0.0
217,194 - OKFHMN_01540 0.49 -2.9
217,420 - +0.3
218,140 + +0.6
218,174 + +0.6
218,360 + +0.6
218,361 - -1.4
218,651 - patD OKFHMN_01555 0.25 -1.4
218,651 - patD OKFHMN_01555 0.25 -1.9
218,653 + patD OKFHMN_01555 0.25 -0.4

Or see this region's nucleotide sequence