Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybdR and uspG are separated by 220 nucleotides uspG and BNILDI_21890 are separated by 120 nucleotides BNILDI_21890 and ahpC are separated by 98 nucleotides
BNILDI_21880: ybdR - Uncharacterized zinc-type alcohol dehydrogenase-like protein YbdR, at 4,396,579 to 4,397,817
ybdR
BNILDI_21885: uspG - universal stress protein UspG, at 4,398,038 to 4,398,466
uspG
BNILDI_21890: BNILDI_21890 - Alkyl hydroperoxide reductase subunit F, at 4,398,587 to 4,400,182
_21890
BNILDI_21895: ahpC - alkyl hydroperoxide reductase subunit C, at 4,400,281 to 4,400,844
ahpC
Position (kb)
4398
4399
4400
4401 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4397.734 kb on - strand at 4397.736 kb on + strand at 4397.801 kb on + strand at 4397.861 kb on + strand at 4397.861 kb on + strand at 4397.862 kb on - strand at 4397.862 kb on - strand at 4397.928 kb on + strand at 4397.928 kb on + strand at 4397.930 kb on + strand at 4397.931 kb on - strand at 4397.931 kb on - strand at 4398.041 kb on + strand at 4398.041 kb on + strand at 4398.041 kb on + strand at 4398.041 kb on + strand at 4398.042 kb on - strand at 4398.042 kb on - strand at 4398.043 kb on + strand at 4398.144 kb on + strand, within uspG at 4398.144 kb on + strand, within uspG at 4398.145 kb on - strand, within uspG at 4398.153 kb on + strand, within uspG at 4398.154 kb on - strand, within uspG at 4398.367 kb on + strand, within uspG at 4398.368 kb on - strand, within uspG at 4398.368 kb on - strand, within uspG at 4398.465 kb on - strand at 4398.476 kb on + strand at 4398.476 kb on + strand at 4398.743 kb on - strand at 4398.928 kb on + strand, within BNILDI_21890 at 4399.123 kb on - strand, within BNILDI_21890 at 4399.454 kb on - strand, within BNILDI_21890 at 4399.508 kb on + strand, within BNILDI_21890 at 4399.508 kb on + strand, within BNILDI_21890 at 4399.509 kb on - strand, within BNILDI_21890 at 4399.515 kb on + strand, within BNILDI_21890 at 4399.626 kb on - strand, within BNILDI_21890 at 4399.780 kb on - strand, within BNILDI_21890 at 4399.780 kb on - strand, within BNILDI_21890 at 4399.988 kb on - strand, within BNILDI_21890 at 4399.988 kb on - strand, within BNILDI_21890 at 4400.060 kb on - strand at 4400.060 kb on - strand at 4400.085 kb on - strand at 4400.115 kb on + strand at 4400.262 kb on - strand at 4400.266 kb on - strand at 4400.290 kb on - strand at 4400.371 kb on + strand, within ahpC at 4400.371 kb on + strand, within ahpC at 4400.372 kb on - strand, within ahpC at 4400.372 kb on - strand, within ahpC at 4400.372 kb on - strand, within ahpC at 4400.629 kb on - strand, within ahpC at 4400.642 kb on - strand, within ahpC at 4400.642 kb on - strand, within ahpC at 4400.821 kb on - strand at 4400.833 kb on - strand at 4400.875 kb on + strand at 4400.875 kb on + strand at 4400.875 kb on + strand at 4400.875 kb on + strand at 4400.876 kb on - strand at 4400.876 kb on - strand at 4400.876 kb on - strand at 4400.876 kb on - strand at 4400.876 kb on - strand at 4400.876 kb on - strand at 4400.876 kb on - strand at 4400.940 kb on + strand at 4400.996 kb on - strand at 4401.024 kb on + strand at 4401.025 kb on - strand at 4401.045 kb on - strand at 4401.082 kb on + strand at 4401.107 kb on - strand at 4401.119 kb on + strand at 4401.143 kb on + strand at 4401.143 kb on + strand at 4401.144 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,397,734 - +1.1 4,397,736 + +1.8 4,397,801 + +0.6 4,397,861 + -1.4 4,397,861 + -2.0 4,397,862 - -0.3 4,397,862 - +0.7 4,397,928 + -2.7 4,397,928 + -0.4 4,397,930 + +0.1 4,397,931 - -0.6 4,397,931 - -0.4 4,398,041 + +0.1 4,398,041 + +1.7 4,398,041 + +0.6 4,398,041 + +1.4 4,398,042 - +0.6 4,398,042 - +1.1 4,398,043 + -0.9 4,398,144 + uspG BNILDI_21885 0.25 -0.5 4,398,144 + uspG BNILDI_21885 0.25 -1.7 4,398,145 - uspG BNILDI_21885 0.25 -2.1 4,398,153 + uspG BNILDI_21885 0.27 +0.2 4,398,154 - uspG BNILDI_21885 0.27 +0.0 4,398,367 + uspG BNILDI_21885 0.77 -0.8 4,398,368 - uspG BNILDI_21885 0.77 +1.6 4,398,368 - uspG BNILDI_21885 0.77 -2.3 4,398,465 - +0.4 4,398,476 + -0.1 4,398,476 + +1.7 4,398,743 - +0.7 4,398,928 + BNILDI_21890 0.21 -0.1 4,399,123 - BNILDI_21890 0.34 +1.6 4,399,454 - BNILDI_21890 0.54 -0.2 4,399,508 + BNILDI_21890 0.58 -0.6 4,399,508 + BNILDI_21890 0.58 +0.5 4,399,509 - BNILDI_21890 0.58 +1.3 4,399,515 + BNILDI_21890 0.58 +1.3 4,399,626 - BNILDI_21890 0.65 -0.6 4,399,780 - BNILDI_21890 0.75 +1.0 4,399,780 - BNILDI_21890 0.75 -0.9 4,399,988 - BNILDI_21890 0.88 +0.4 4,399,988 - BNILDI_21890 0.88 +1.0 4,400,060 - -3.2 4,400,060 - +1.0 4,400,085 - +0.1 4,400,115 + -1.3 4,400,262 - +0.2 4,400,266 - -0.2 4,400,290 - +0.7 4,400,371 + ahpC BNILDI_21895 0.16 +0.3 4,400,371 + ahpC BNILDI_21895 0.16 -0.4 4,400,372 - ahpC BNILDI_21895 0.16 -0.2 4,400,372 - ahpC BNILDI_21895 0.16 -1.1 4,400,372 - ahpC BNILDI_21895 0.16 -1.2 4,400,629 - ahpC BNILDI_21895 0.62 -0.4 4,400,642 - ahpC BNILDI_21895 0.64 -0.7 4,400,642 - ahpC BNILDI_21895 0.64 +2.3 4,400,821 - -2.2 4,400,833 - -0.9 4,400,875 + +0.2 4,400,875 + +1.3 4,400,875 + +0.2 4,400,875 + +0.1 4,400,876 - -1.4 4,400,876 - +0.7 4,400,876 - -0.1 4,400,876 - -1.2 4,400,876 - -3.1 4,400,876 - +0.2 4,400,876 - -0.0 4,400,940 + -0.6 4,400,996 - +0.7 4,401,024 + +0.7 4,401,025 - +0.0 4,401,045 - +0.7 4,401,082 + +0.6 4,401,107 - -0.3 4,401,119 + +1.8 4,401,143 + +1.9 4,401,143 + +0.4 4,401,144 - +0.2
Or see this region's nucleotide sequence