Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybeU and ybeT are separated by 100 nucleotides ybeT and djlB are separated by 9 nucleotides djlB and ybeR overlap by 4 nucleotides ybeR and ybeQ are separated by 163 nucleotides
BNILDI_21685: ybeU - Uncharacterized protein YbeU, at 4,359,687 to 4,360,394
ybeU
BNILDI_21690: ybeT - Sel1-repeat-containing protein YbeT, at 4,360,495 to 4,361,049
ybeT
BNILDI_21695: djlB - Uncharacterized J domain-containing protein DjlB, at 4,361,059 to 4,362,486
djlB
BNILDI_21700: ybeR - Uncharacterized protein YbeR, at 4,362,483 to 4,363,190
ybeR
BNILDI_21705: ybeQ - Sel1-repeat-containing protein YbeQ, at 4,363,354 to 4,364,331
ybeQ
Position (kb)
4361
4362
4363 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4360.077 kb on - strand, within ybeU at 4360.177 kb on + strand, within ybeU at 4360.178 kb on - strand, within ybeU at 4360.617 kb on - strand, within ybeT at 4360.745 kb on - strand, within ybeT at 4360.745 kb on - strand, within ybeT at 4360.746 kb on + strand, within ybeT at 4360.765 kb on + strand, within ybeT at 4360.766 kb on - strand, within ybeT at 4360.822 kb on + strand, within ybeT at 4360.897 kb on + strand, within ybeT at 4360.951 kb on - strand, within ybeT at 4361.228 kb on - strand, within djlB at 4361.245 kb on + strand, within djlB at 4361.245 kb on + strand, within djlB at 4361.245 kb on + strand, within djlB at 4361.348 kb on - strand, within djlB at 4361.516 kb on + strand, within djlB at 4361.516 kb on + strand, within djlB at 4361.516 kb on + strand, within djlB at 4361.809 kb on - strand, within djlB at 4361.811 kb on - strand, within djlB at 4361.880 kb on - strand, within djlB at 4362.014 kb on + strand, within djlB at 4362.014 kb on + strand, within djlB at 4362.014 kb on + strand, within djlB at 4362.088 kb on + strand, within djlB at 4362.089 kb on - strand, within djlB at 4362.112 kb on + strand, within djlB at 4362.247 kb on + strand, within djlB at 4362.248 kb on - strand, within djlB at 4362.291 kb on - strand, within djlB at 4362.560 kb on - strand, within ybeR at 4362.583 kb on - strand, within ybeR at 4362.645 kb on + strand, within ybeR at 4362.695 kb on + strand, within ybeR at 4362.735 kb on + strand, within ybeR at 4362.802 kb on - strand, within ybeR at 4362.931 kb on + strand, within ybeR at 4363.086 kb on - strand, within ybeR at 4363.090 kb on - strand, within ybeR at 4363.119 kb on + strand, within ybeR at 4363.438 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,360,077 - ybeU BNILDI_21685 0.55 -2.5 4,360,177 + ybeU BNILDI_21685 0.69 -1.2 4,360,178 - ybeU BNILDI_21685 0.69 -0.2 4,360,617 - ybeT BNILDI_21690 0.22 -0.5 4,360,745 - ybeT BNILDI_21690 0.45 -2.2 4,360,745 - ybeT BNILDI_21690 0.45 +0.2 4,360,746 + ybeT BNILDI_21690 0.45 -0.5 4,360,765 + ybeT BNILDI_21690 0.49 +2.2 4,360,766 - ybeT BNILDI_21690 0.49 -0.7 4,360,822 + ybeT BNILDI_21690 0.59 +1.5 4,360,897 + ybeT BNILDI_21690 0.72 -3.5 4,360,951 - ybeT BNILDI_21690 0.82 -0.6 4,361,228 - djlB BNILDI_21695 0.12 -3.4 4,361,245 + djlB BNILDI_21695 0.13 +0.8 4,361,245 + djlB BNILDI_21695 0.13 +0.7 4,361,245 + djlB BNILDI_21695 0.13 +0.4 4,361,348 - djlB BNILDI_21695 0.20 +0.9 4,361,516 + djlB BNILDI_21695 0.32 -0.6 4,361,516 + djlB BNILDI_21695 0.32 +0.5 4,361,516 + djlB BNILDI_21695 0.32 +1.4 4,361,809 - djlB BNILDI_21695 0.53 +0.4 4,361,811 - djlB BNILDI_21695 0.53 -1.1 4,361,880 - djlB BNILDI_21695 0.57 -0.7 4,362,014 + djlB BNILDI_21695 0.67 +1.0 4,362,014 + djlB BNILDI_21695 0.67 -0.3 4,362,014 + djlB BNILDI_21695 0.67 +0.5 4,362,088 + djlB BNILDI_21695 0.72 -0.8 4,362,089 - djlB BNILDI_21695 0.72 -1.0 4,362,112 + djlB BNILDI_21695 0.74 +0.3 4,362,247 + djlB BNILDI_21695 0.83 -0.0 4,362,248 - djlB BNILDI_21695 0.83 -2.0 4,362,291 - djlB BNILDI_21695 0.86 +1.4 4,362,560 - ybeR BNILDI_21700 0.11 +0.4 4,362,583 - ybeR BNILDI_21700 0.14 +2.2 4,362,645 + ybeR BNILDI_21700 0.23 +0.2 4,362,695 + ybeR BNILDI_21700 0.30 -0.2 4,362,735 + ybeR BNILDI_21700 0.36 -0.0 4,362,802 - ybeR BNILDI_21700 0.45 +0.0 4,362,931 + ybeR BNILDI_21700 0.63 -0.1 4,363,086 - ybeR BNILDI_21700 0.85 -1.7 4,363,090 - ybeR BNILDI_21700 0.86 +0.6 4,363,119 + ybeR BNILDI_21700 0.90 -0.8 4,363,438 + -0.4
Or see this region's nucleotide sequence