Strain Fitness in Escherichia coli ECRC62 around BNILDI_21590

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntasnB and Met_trna are separated by 380 nucleotidesMet_trna and Leu_trna are separated by 8 nucleotidesLeu_trna and Gln_trna are separated by 23 nucleotidesGln_trna and Gln_trna are separated by 34 nucleotidesGln_trna and Met_trna are separated by 15 nucleotidesMet_trna and Gln_trna are separated by 48 nucleotidesGln_trna and Gln_trna are separated by 37 nucleotidesGln_trna and ubiF are separated by 153 nucleotides BNILDI_21565: asnB - asparagine synthase B, at 4,339,911 to 4,341,575 asnB BNILDI_21570: Met_trna - tRNA-Met(cat), at 4,341,956 to 4,342,032 _trna BNILDI_21575: Leu_trna - tRNA-Leu(tag), at 4,342,041 to 4,342,125 _trna BNILDI_21580: Gln_trna - tRNA-Gln(ttg), at 4,342,149 to 4,342,223 _trna BNILDI_21585: Gln_trna - tRNA-Gln(ttg), at 4,342,258 to 4,342,332 _trna BNILDI_21590: Met_trna - tRNA-Met(cat), at 4,342,348 to 4,342,424 _trna BNILDI_21595: Gln_trna - tRNA-Gln(ctg), at 4,342,473 to 4,342,547 _trna BNILDI_21600: Gln_trna - tRNA-Gln(ctg), at 4,342,585 to 4,342,659 _trna BNILDI_21605: ubiF - 3-demethoxyubiquinol 3-hydroxylase, at 4,342,813 to 4,343,988 ubiF Position (kb) 4342 4343Strain fitness (log2 ratio) -2 -1 0 1at 4341.396 kb on + strand, within asnBat 4341.397 kb on - strand, within asnBat 4341.567 kb on + strandat 4341.570 kb on - strandat 4341.574 kb on - strandat 4341.649 kb on + strandat 4341.733 kb on + strandat 4341.809 kb on + strandat 4341.810 kb on - strandat 4341.821 kb on - strandat 4341.821 kb on - strandat 4341.845 kb on - strandat 4342.356 kb on - strand, within Met_trnaat 4342.443 kb on + strandat 4342.564 kb on + strandat 4342.564 kb on + strandat 4342.564 kb on + strandat 4342.565 kb on - strandat 4342.571 kb on + strandat 4342.663 kb on + strandat 4342.780 kb on + strandat 4342.781 kb on - strandat 4342.781 kb on - strandat 4342.809 kb on - strandat 4342.809 kb on - strandat 4342.814 kb on + strandat 4342.815 kb on - strandat 4342.830 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,341,396 + asnB BNILDI_21565 0.89 +0.3
4,341,397 - asnB BNILDI_21565 0.89 +0.3
4,341,567 + +0.1
4,341,570 - +0.7
4,341,574 - -0.0
4,341,649 + +0.2
4,341,733 + -1.6
4,341,809 + -0.7
4,341,810 - +0.3
4,341,821 - -0.8
4,341,821 - -1.1
4,341,845 - +0.3
4,342,356 - Met_trna BNILDI_21590 0.10 -1.1
4,342,443 + -0.6
4,342,564 + -1.6
4,342,564 + -0.4
4,342,564 + -0.2
4,342,565 - +0.0
4,342,571 + -2.0
4,342,663 + -0.1
4,342,780 + -1.0
4,342,781 - -2.0
4,342,781 - -0.0
4,342,809 - +0.5
4,342,809 - -0.4
4,342,814 + -0.1
4,342,815 - -0.1
4,342,830 - -0.8

Or see this region's nucleotide sequence