Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt asnB and Met_trna are separated by 380 nucleotides Met_trna and Leu_trna are separated by 8 nucleotides Leu_trna and Gln_trna are separated by 23 nucleotides Gln_trna and Gln_trna are separated by 34 nucleotides Gln_trna and Met_trna are separated by 15 nucleotides Met_trna and Gln_trna are separated by 48 nucleotides Gln_trna and Gln_trna are separated by 37 nucleotides Gln_trna and ubiF are separated by 153 nucleotides
BNILDI_21565: asnB - asparagine synthase B, at 4,339,911 to 4,341,575
asnB
BNILDI_21570: Met_trna - tRNA-Met(cat), at 4,341,956 to 4,342,032
_trna
BNILDI_21575: Leu_trna - tRNA-Leu(tag), at 4,342,041 to 4,342,125
_trna
BNILDI_21580: Gln_trna - tRNA-Gln(ttg), at 4,342,149 to 4,342,223
_trna
BNILDI_21585: Gln_trna - tRNA-Gln(ttg), at 4,342,258 to 4,342,332
_trna
BNILDI_21590: Met_trna - tRNA-Met(cat), at 4,342,348 to 4,342,424
_trna
BNILDI_21595: Gln_trna - tRNA-Gln(ctg), at 4,342,473 to 4,342,547
_trna
BNILDI_21600: Gln_trna - tRNA-Gln(ctg), at 4,342,585 to 4,342,659
_trna
BNILDI_21605: ubiF - 3-demethoxyubiquinol 3-hydroxylase, at 4,342,813 to 4,343,988
ubiF
Position (kb)
4342
4343 Strain fitness (log2 ratio)
-2
-1
0
1 at 4341.396 kb on + strand, within asnB at 4341.397 kb on - strand, within asnB at 4341.567 kb on + strand at 4341.570 kb on - strand at 4341.574 kb on - strand at 4341.649 kb on + strand at 4341.733 kb on + strand at 4341.809 kb on + strand at 4341.810 kb on - strand at 4341.821 kb on - strand at 4341.821 kb on - strand at 4341.845 kb on - strand at 4342.356 kb on - strand, within Met_trna at 4342.443 kb on + strand at 4342.564 kb on + strand at 4342.564 kb on + strand at 4342.564 kb on + strand at 4342.565 kb on - strand at 4342.571 kb on + strand at 4342.663 kb on + strand at 4342.780 kb on + strand at 4342.781 kb on - strand at 4342.781 kb on - strand at 4342.809 kb on - strand at 4342.809 kb on - strand at 4342.814 kb on + strand at 4342.815 kb on - strand at 4342.830 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,341,396 + asnB BNILDI_21565 0.89 +0.3 4,341,397 - asnB BNILDI_21565 0.89 +0.3 4,341,567 + +0.1 4,341,570 - +0.7 4,341,574 - -0.0 4,341,649 + +0.2 4,341,733 + -1.6 4,341,809 + -0.7 4,341,810 - +0.3 4,341,821 - -0.8 4,341,821 - -1.1 4,341,845 - +0.3 4,342,356 - Met_trna BNILDI_21590 0.10 -1.1 4,342,443 + -0.6 4,342,564 + -1.6 4,342,564 + -0.4 4,342,564 + -0.2 4,342,565 - +0.0 4,342,571 + -2.0 4,342,663 + -0.1 4,342,780 + -1.0 4,342,781 - -2.0 4,342,781 - -0.0 4,342,809 - +0.5 4,342,809 - -0.4 4,342,814 + -0.1 4,342,815 - -0.1 4,342,830 - -0.8
Or see this region's nucleotide sequence