Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucA and sdhB are separated by 200 nucleotides sdhB and sdhA are separated by 15 nucleotides
BNILDI_21310: sucA - 2-oxoglutarate dehydrogenase E1 component, at 4,284,263 to 4,287,064
sucA
BNILDI_21315: sdhB - succinate dehydrogenase iron-sulfur subunit SdhB, at 4,287,265 to 4,287,981
sdhB
BNILDI_21320: sdhA - succinate dehydrogenase flavoprotein subunit, at 4,287,997 to 4,289,763
sdhA
Position (kb)
4287
4288 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2
3 at 4286.548 kb on + strand, within sucA at 4286.548 kb on + strand, within sucA at 4286.551 kb on - strand, within sucA at 4287.025 kb on - strand at 4287.095 kb on - strand at 4287.095 kb on - strand at 4287.125 kb on - strand at 4287.263 kb on - strand at 4287.263 kb on - strand at 4287.266 kb on + strand at 4287.267 kb on - strand at 4287.274 kb on - strand at 4287.337 kb on + strand, within sdhB at 4287.338 kb on - strand, within sdhB at 4287.534 kb on + strand, within sdhB at 4287.624 kb on - strand, within sdhB at 4287.753 kb on + strand, within sdhB at 4287.914 kb on + strand at 4287.915 kb on - strand at 4287.915 kb on - strand at 4287.959 kb on + strand at 4287.998 kb on + strand at 4288.001 kb on + strand at 4288.002 kb on - strand at 4288.002 kb on - strand at 4288.006 kb on - strand at 4288.356 kb on + strand, within sdhA at 4288.357 kb on - strand, within sdhA at 4288.365 kb on + strand, within sdhA at 4288.366 kb on - strand, within sdhA at 4288.366 kb on - strand, within sdhA at 4288.366 kb on - strand, within sdhA at 4288.700 kb on + strand, within sdhA at 4288.701 kb on - strand, within sdhA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,286,548 + sucA BNILDI_21310 0.82 -2.6 4,286,548 + sucA BNILDI_21310 0.82 -3.1 4,286,551 - sucA BNILDI_21310 0.82 +3.0 4,287,025 - -2.4 4,287,095 - -1.0 4,287,095 - -3.5 4,287,125 - -7.2 4,287,263 - -0.3 4,287,263 - -0.8 4,287,266 + -0.6 4,287,267 - -0.5 4,287,274 - -2.6 4,287,337 + sdhB BNILDI_21315 0.10 -0.0 4,287,338 - sdhB BNILDI_21315 0.10 -0.5 4,287,534 + sdhB BNILDI_21315 0.38 -2.1 4,287,624 - sdhB BNILDI_21315 0.50 -3.0 4,287,753 + sdhB BNILDI_21315 0.68 -2.7 4,287,914 + -1.9 4,287,915 - -1.6 4,287,915 - -2.8 4,287,959 + -4.1 4,287,998 + -3.5 4,288,001 + -3.5 4,288,002 - -2.7 4,288,002 - -0.6 4,288,006 - -2.7 4,288,356 + sdhA BNILDI_21320 0.20 -2.2 4,288,357 - sdhA BNILDI_21320 0.20 -1.8 4,288,365 + sdhA BNILDI_21320 0.21 -4.1 4,288,366 - sdhA BNILDI_21320 0.21 -3.3 4,288,366 - sdhA BNILDI_21320 0.21 +0.2 4,288,366 - sdhA BNILDI_21320 0.21 -2.1 4,288,700 + sdhA BNILDI_21320 0.40 -0.9 4,288,701 - sdhA BNILDI_21320 0.40 -1.3
Or see this region's nucleotide sequence