Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mngR and sucD are separated by 103 nucleotides sucD and sucC overlap by 1 nucleotides sucC and odhB are separated by 93 nucleotides
BNILDI_21290: mngR - mannosyl-D-glycerate transport/metabolism system transcriptional repressor MngR, at 4,280,076 to 4,280,798
mngR
BNILDI_21295: sucD - succinate--CoA ligase subunit alpha, at 4,280,902 to 4,281,771
sucD
BNILDI_21300: sucC - ADP-forming succinate--CoA ligase subunit beta, at 4,281,771 to 4,282,937
sucC
BNILDI_21305: odhB - 2-oxoglutarate dehydrogenase complex dihydrolipoyllysine-residue succinyltransferase, at 4,283,031 to 4,284,248
odhB
Position (kb)
4281
4282
4283 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4280.817 kb on + strand at 4280.817 kb on + strand at 4280.884 kb on - strand at 4280.884 kb on - strand at 4280.899 kb on + strand at 4280.904 kb on - strand at 4281.039 kb on + strand, within sucD at 4281.074 kb on + strand, within sucD at 4281.075 kb on - strand, within sucD at 4281.110 kb on + strand, within sucD at 4281.157 kb on + strand, within sucD at 4281.417 kb on + strand, within sucD at 4281.418 kb on - strand, within sucD at 4281.516 kb on + strand, within sucD at 4281.553 kb on + strand, within sucD at 4281.557 kb on + strand, within sucD at 4281.557 kb on + strand, within sucD at 4281.557 kb on + strand, within sucD at 4281.557 kb on + strand, within sucD at 4281.558 kb on - strand, within sucD at 4281.558 kb on - strand, within sucD at 4281.558 kb on - strand, within sucD at 4281.558 kb on - strand, within sucD at 4281.673 kb on - strand, within sucD at 4281.673 kb on - strand, within sucD at 4281.772 kb on + strand at 4281.772 kb on + strand at 4282.154 kb on + strand, within sucC at 4282.154 kb on + strand, within sucC at 4282.154 kb on + strand, within sucC at 4282.155 kb on - strand, within sucC at 4282.155 kb on - strand, within sucC at 4282.155 kb on - strand, within sucC at 4282.184 kb on - strand, within sucC at 4282.590 kb on - strand, within sucC at 4282.612 kb on + strand, within sucC at 4282.688 kb on - strand, within sucC at 4282.860 kb on - strand at 4282.870 kb on + strand at 4282.929 kb on + strand at 4282.930 kb on - strand at 4282.980 kb on - strand at 4283.006 kb on + strand at 4283.007 kb on - strand at 4283.024 kb on + strand at 4283.033 kb on - strand at 4283.247 kb on - strand, within odhB at 4283.453 kb on - strand, within odhB at 4283.591 kb on - strand, within odhB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,280,817 + +0.1 4,280,817 + +0.3 4,280,884 - -0.0 4,280,884 - +0.3 4,280,899 + -2.8 4,280,904 - -0.4 4,281,039 + sucD BNILDI_21295 0.16 -0.6 4,281,074 + sucD BNILDI_21295 0.20 -2.0 4,281,075 - sucD BNILDI_21295 0.20 -4.2 4,281,110 + sucD BNILDI_21295 0.24 -1.2 4,281,157 + sucD BNILDI_21295 0.29 -3.1 4,281,417 + sucD BNILDI_21295 0.59 -3.5 4,281,418 - sucD BNILDI_21295 0.59 -3.2 4,281,516 + sucD BNILDI_21295 0.71 -3.8 4,281,553 + sucD BNILDI_21295 0.75 -2.4 4,281,557 + sucD BNILDI_21295 0.75 +0.4 4,281,557 + sucD BNILDI_21295 0.75 -4.2 4,281,557 + sucD BNILDI_21295 0.75 -2.7 4,281,557 + sucD BNILDI_21295 0.75 -5.2 4,281,558 - sucD BNILDI_21295 0.75 -2.7 4,281,558 - sucD BNILDI_21295 0.75 -2.1 4,281,558 - sucD BNILDI_21295 0.75 -2.4 4,281,558 - sucD BNILDI_21295 0.75 -3.8 4,281,673 - sucD BNILDI_21295 0.89 -2.7 4,281,673 - sucD BNILDI_21295 0.89 -1.6 4,281,772 + -1.6 4,281,772 + -5.6 4,282,154 + sucC BNILDI_21300 0.33 -1.5 4,282,154 + sucC BNILDI_21300 0.33 -4.0 4,282,154 + sucC BNILDI_21300 0.33 -1.6 4,282,155 - sucC BNILDI_21300 0.33 -2.1 4,282,155 - sucC BNILDI_21300 0.33 +0.9 4,282,155 - sucC BNILDI_21300 0.33 -3.0 4,282,184 - sucC BNILDI_21300 0.35 -5.1 4,282,590 - sucC BNILDI_21300 0.70 -3.3 4,282,612 + sucC BNILDI_21300 0.72 -0.4 4,282,688 - sucC BNILDI_21300 0.79 -4.1 4,282,860 - +0.2 4,282,870 + -2.2 4,282,929 + -2.6 4,282,930 - -3.0 4,282,980 - -3.8 4,283,006 + -1.5 4,283,007 - +0.3 4,283,024 + -4.5 4,283,033 - -2.6 4,283,247 - odhB BNILDI_21305 0.18 -2.3 4,283,453 - odhB BNILDI_21305 0.35 -1.5 4,283,591 - odhB BNILDI_21305 0.46 -0.6
Or see this region's nucleotide sequence