Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioB and bioA are separated by 86 nucleotides bioA and ybhB are separated by 58 nucleotides
BNILDI_21040: bioB - biotin synthase BioB, at 4,233,954 to 4,234,994
bioB
BNILDI_21045: bioA - adenosylmethionine--8-amino-7-oxononanoate transaminase, at 4,235,081 to 4,236,370
bioA
BNILDI_21050: ybhB - kinase inhibitor, at 4,236,429 to 4,236,905
ybhB
Position (kb) 4235
4236
4237 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 4234.505 kb on + strand, within bioB at 4234.505 kb on + strand, within bioB at 4234.547 kb on - strand, within bioB at 4234.547 kb on - strand, within bioB at 4234.547 kb on - strand, within bioB at 4234.547 kb on - strand, within bioB at 4234.549 kb on + strand, within bioB at 4234.550 kb on - strand, within bioB at 4234.611 kb on - strand, within bioB at 4235.025 kb on + strand at 4235.025 kb on + strand at 4235.129 kb on + strand at 4235.190 kb on + strand at 4235.224 kb on + strand, within bioA at 4235.224 kb on + strand, within bioA at 4235.224 kb on + strand, within bioA at 4235.273 kb on - strand, within bioA at 4235.273 kb on - strand, within bioA at 4235.510 kb on + strand, within bioA at 4235.583 kb on - strand, within bioA at 4235.747 kb on + strand, within bioA at 4235.747 kb on + strand, within bioA at 4235.747 kb on + strand, within bioA at 4235.748 kb on - strand, within bioA at 4235.798 kb on - strand, within bioA at 4235.864 kb on + strand, within bioA at 4235.992 kb on + strand, within bioA at 4236.102 kb on + strand, within bioA at 4236.103 kb on - strand, within bioA at 4236.103 kb on - strand, within bioA at 4236.289 kb on + strand at 4236.293 kb on - strand at 4236.443 kb on + strand at 4236.465 kb on - strand at 4236.515 kb on + strand, within ybhB at 4236.516 kb on - strand, within ybhB at 4236.623 kb on - strand, within ybhB at 4236.623 kb on - strand, within ybhB at 4236.630 kb on - strand, within ybhB at 4236.782 kb on + strand, within ybhB at 4236.802 kb on + strand, within ybhB at 4236.802 kb on + strand, within ybhB at 4236.803 kb on - strand, within ybhB at 4236.803 kb on - strand, within ybhB at 4236.810 kb on - strand, within ybhB at 4236.810 kb on - strand, within ybhB at 4236.810 kb on - strand, within ybhB at 4236.899 kb on + strand at 4236.904 kb on - strand at 4236.938 kb on - strand at 4236.944 kb on + strand at 4236.945 kb on - strand at 4236.951 kb on - strand at 4236.953 kb on + strand at 4236.954 kb on - strand at 4237.077 kb on + strand at 4237.101 kb on + strand at 4237.128 kb on - strand at 4237.285 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,234,505 + bioB BNILDI_21040 0.53 -2.2 4,234,505 + bioB BNILDI_21040 0.53 -1.2 4,234,547 - bioB BNILDI_21040 0.57 -1.9 4,234,547 - bioB BNILDI_21040 0.57 -1.4 4,234,547 - bioB BNILDI_21040 0.57 -0.8 4,234,547 - bioB BNILDI_21040 0.57 +0.4 4,234,549 + bioB BNILDI_21040 0.57 -0.6 4,234,550 - bioB BNILDI_21040 0.57 -2.8 4,234,611 - bioB BNILDI_21040 0.63 -2.0 4,235,025 + -0.4 4,235,025 + -0.3 4,235,129 + -2.9 4,235,190 + -0.5 4,235,224 + bioA BNILDI_21045 0.11 +0.0 4,235,224 + bioA BNILDI_21045 0.11 -0.1 4,235,224 + bioA BNILDI_21045 0.11 +0.4 4,235,273 - bioA BNILDI_21045 0.15 -1.1 4,235,273 - bioA BNILDI_21045 0.15 +0.7 4,235,510 + bioA BNILDI_21045 0.33 -0.6 4,235,583 - bioA BNILDI_21045 0.39 -0.6 4,235,747 + bioA BNILDI_21045 0.52 +0.5 4,235,747 + bioA BNILDI_21045 0.52 +0.7 4,235,747 + bioA BNILDI_21045 0.52 -0.2 4,235,748 - bioA BNILDI_21045 0.52 -0.3 4,235,798 - bioA BNILDI_21045 0.56 -0.1 4,235,864 + bioA BNILDI_21045 0.61 +0.2 4,235,992 + bioA BNILDI_21045 0.71 -2.4 4,236,102 + bioA BNILDI_21045 0.79 +0.3 4,236,103 - bioA BNILDI_21045 0.79 +0.1 4,236,103 - bioA BNILDI_21045 0.79 -0.9 4,236,289 + +1.1 4,236,293 - -2.0 4,236,443 + +2.0 4,236,465 - -0.2 4,236,515 + ybhB BNILDI_21050 0.18 -1.2 4,236,516 - ybhB BNILDI_21050 0.18 -0.4 4,236,623 - ybhB BNILDI_21050 0.41 +0.1 4,236,623 - ybhB BNILDI_21050 0.41 -5.2 4,236,630 - ybhB BNILDI_21050 0.42 -0.1 4,236,782 + ybhB BNILDI_21050 0.74 -0.2 4,236,802 + ybhB BNILDI_21050 0.78 -0.0 4,236,802 + ybhB BNILDI_21050 0.78 -0.1 4,236,803 - ybhB BNILDI_21050 0.78 -2.4 4,236,803 - ybhB BNILDI_21050 0.78 -3.1 4,236,810 - ybhB BNILDI_21050 0.80 -2.1 4,236,810 - ybhB BNILDI_21050 0.80 +0.5 4,236,810 - ybhB BNILDI_21050 0.80 +0.3 4,236,899 + -0.4 4,236,904 - -0.1 4,236,938 - -0.6 4,236,944 + +0.7 4,236,945 - +0.8 4,236,951 - -2.5 4,236,953 + -1.0 4,236,954 - -0.2 4,237,077 + +0.5 4,237,101 + -2.7 4,237,128 - -0.9 4,237,285 - +0.7
Or see this region's nucleotide sequence