Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioF and bioB overlap by 4 nucleotides bioB and bioA are separated by 86 nucleotides
BNILDI_21035: bioF - 8-amino-7-oxononanoate synthase, at 4,232,803 to 4,233,957
bioF
BNILDI_21040: bioB - biotin synthase BioB, at 4,233,954 to 4,234,994
bioB
BNILDI_21045: bioA - adenosylmethionine--8-amino-7-oxononanoate transaminase, at 4,235,081 to 4,236,370
bioA
Position (kb) 4233
4234
4235 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4233.167 kb on + strand, within bioF at 4233.167 kb on + strand, within bioF at 4233.167 kb on + strand, within bioF at 4233.168 kb on - strand, within bioF at 4233.209 kb on + strand, within bioF at 4233.210 kb on + strand, within bioF at 4233.210 kb on + strand, within bioF at 4233.210 kb on + strand, within bioF at 4233.211 kb on - strand, within bioF at 4233.211 kb on - strand, within bioF at 4233.211 kb on - strand, within bioF at 4233.380 kb on - strand, within bioF at 4233.532 kb on + strand, within bioF at 4233.647 kb on - strand, within bioF at 4233.647 kb on - strand, within bioF at 4233.711 kb on + strand, within bioF at 4233.802 kb on + strand, within bioF at 4233.803 kb on - strand, within bioF at 4233.812 kb on + strand, within bioF at 4233.813 kb on - strand, within bioF at 4233.958 kb on - strand at 4233.958 kb on - strand at 4233.973 kb on + strand at 4234.505 kb on + strand, within bioB at 4234.505 kb on + strand, within bioB at 4234.547 kb on - strand, within bioB at 4234.547 kb on - strand, within bioB at 4234.547 kb on - strand, within bioB at 4234.547 kb on - strand, within bioB at 4234.549 kb on + strand, within bioB at 4234.550 kb on - strand, within bioB at 4234.611 kb on - strand, within bioB at 4235.025 kb on + strand at 4235.025 kb on + strand at 4235.129 kb on + strand at 4235.190 kb on + strand at 4235.224 kb on + strand, within bioA at 4235.224 kb on + strand, within bioA at 4235.224 kb on + strand, within bioA at 4235.273 kb on - strand, within bioA at 4235.273 kb on - strand, within bioA at 4235.510 kb on + strand, within bioA at 4235.583 kb on - strand, within bioA at 4235.747 kb on + strand, within bioA at 4235.747 kb on + strand, within bioA at 4235.747 kb on + strand, within bioA at 4235.748 kb on - strand, within bioA at 4235.798 kb on - strand, within bioA at 4235.864 kb on + strand, within bioA at 4235.992 kb on + strand, within bioA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,233,167 + bioF BNILDI_21035 0.32 -1.8 4,233,167 + bioF BNILDI_21035 0.32 -1.4 4,233,167 + bioF BNILDI_21035 0.32 -1.0 4,233,168 - bioF BNILDI_21035 0.32 -0.6 4,233,209 + bioF BNILDI_21035 0.35 -1.2 4,233,210 + bioF BNILDI_21035 0.35 -0.5 4,233,210 + bioF BNILDI_21035 0.35 -0.7 4,233,210 + bioF BNILDI_21035 0.35 +1.2 4,233,211 - bioF BNILDI_21035 0.35 -2.0 4,233,211 - bioF BNILDI_21035 0.35 -0.3 4,233,211 - bioF BNILDI_21035 0.35 -0.3 4,233,380 - bioF BNILDI_21035 0.50 -1.8 4,233,532 + bioF BNILDI_21035 0.63 -0.2 4,233,647 - bioF BNILDI_21035 0.73 -0.3 4,233,647 - bioF BNILDI_21035 0.73 -0.9 4,233,711 + bioF BNILDI_21035 0.79 +0.2 4,233,802 + bioF BNILDI_21035 0.86 -1.4 4,233,803 - bioF BNILDI_21035 0.87 -1.1 4,233,812 + bioF BNILDI_21035 0.87 -0.9 4,233,813 - bioF BNILDI_21035 0.87 -1.0 4,233,958 - +0.4 4,233,958 - -0.5 4,233,973 + +1.7 4,234,505 + bioB BNILDI_21040 0.53 -2.2 4,234,505 + bioB BNILDI_21040 0.53 -1.2 4,234,547 - bioB BNILDI_21040 0.57 -1.9 4,234,547 - bioB BNILDI_21040 0.57 -1.4 4,234,547 - bioB BNILDI_21040 0.57 -0.8 4,234,547 - bioB BNILDI_21040 0.57 +0.4 4,234,549 + bioB BNILDI_21040 0.57 -0.6 4,234,550 - bioB BNILDI_21040 0.57 -2.8 4,234,611 - bioB BNILDI_21040 0.63 -2.0 4,235,025 + -0.4 4,235,025 + -0.3 4,235,129 + -2.9 4,235,190 + -0.5 4,235,224 + bioA BNILDI_21045 0.11 +0.0 4,235,224 + bioA BNILDI_21045 0.11 -0.1 4,235,224 + bioA BNILDI_21045 0.11 +0.4 4,235,273 - bioA BNILDI_21045 0.15 -1.1 4,235,273 - bioA BNILDI_21045 0.15 +0.7 4,235,510 + bioA BNILDI_21045 0.33 -0.6 4,235,583 - bioA BNILDI_21045 0.39 -0.6 4,235,747 + bioA BNILDI_21045 0.52 +0.5 4,235,747 + bioA BNILDI_21045 0.52 +0.7 4,235,747 + bioA BNILDI_21045 0.52 -0.2 4,235,748 - bioA BNILDI_21045 0.52 -0.3 4,235,798 - bioA BNILDI_21045 0.56 -0.1 4,235,864 + bioA BNILDI_21045 0.61 +0.2 4,235,992 + bioA BNILDI_21045 0.71 -2.4
Or see this region's nucleotide sequence