Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioD and bioC overlap by 8 nucleotides bioC and bioF overlap by 14 nucleotides bioF and bioB overlap by 4 nucleotides
BNILDI_21025: bioD - dethiobiotin synthase, at 4,231,391 to 4,232,068
bioD
BNILDI_21030: bioC - malonyl-ACP O-methyltransferase BioC, at 4,232,061 to 4,232,816
bioC
BNILDI_21035: bioF - 8-amino-7-oxononanoate synthase, at 4,232,803 to 4,233,957
bioF
BNILDI_21040: bioB - biotin synthase BioB, at 4,233,954 to 4,234,994
bioB
Position (kb)
4232
4233
4234 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4231.893 kb on - strand, within bioD at 4231.912 kb on + strand, within bioD at 4231.912 kb on + strand, within bioD at 4231.913 kb on - strand, within bioD at 4231.913 kb on - strand, within bioD at 4231.913 kb on - strand, within bioD at 4231.913 kb on - strand, within bioD at 4231.957 kb on + strand, within bioD at 4231.960 kb on - strand, within bioD at 4232.095 kb on + strand at 4232.095 kb on + strand at 4232.095 kb on + strand at 4232.267 kb on + strand, within bioC at 4232.607 kb on + strand, within bioC at 4232.607 kb on + strand, within bioC at 4232.608 kb on - strand, within bioC at 4232.689 kb on + strand, within bioC at 4232.689 kb on + strand, within bioC at 4232.693 kb on + strand, within bioC at 4232.694 kb on - strand, within bioC at 4232.739 kb on + strand, within bioC at 4232.739 kb on + strand, within bioC at 4233.167 kb on + strand, within bioF at 4233.167 kb on + strand, within bioF at 4233.167 kb on + strand, within bioF at 4233.168 kb on - strand, within bioF at 4233.209 kb on + strand, within bioF at 4233.210 kb on + strand, within bioF at 4233.210 kb on + strand, within bioF at 4233.210 kb on + strand, within bioF at 4233.211 kb on - strand, within bioF at 4233.211 kb on - strand, within bioF at 4233.211 kb on - strand, within bioF at 4233.380 kb on - strand, within bioF at 4233.532 kb on + strand, within bioF at 4233.647 kb on - strand, within bioF at 4233.647 kb on - strand, within bioF at 4233.711 kb on + strand, within bioF at 4233.802 kb on + strand, within bioF at 4233.803 kb on - strand, within bioF at 4233.812 kb on + strand, within bioF at 4233.813 kb on - strand, within bioF at 4233.958 kb on - strand at 4233.958 kb on - strand at 4233.973 kb on + strand at 4234.505 kb on + strand, within bioB at 4234.505 kb on + strand, within bioB at 4234.547 kb on - strand, within bioB at 4234.547 kb on - strand, within bioB at 4234.547 kb on - strand, within bioB at 4234.547 kb on - strand, within bioB at 4234.549 kb on + strand, within bioB at 4234.550 kb on - strand, within bioB at 4234.611 kb on - strand, within bioB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,231,893 - bioD BNILDI_21025 0.74 +0.1 4,231,912 + bioD BNILDI_21025 0.77 -0.2 4,231,912 + bioD BNILDI_21025 0.77 -0.5 4,231,913 - bioD BNILDI_21025 0.77 -2.0 4,231,913 - bioD BNILDI_21025 0.77 +0.4 4,231,913 - bioD BNILDI_21025 0.77 +0.8 4,231,913 - bioD BNILDI_21025 0.77 -0.3 4,231,957 + bioD BNILDI_21025 0.83 -1.8 4,231,960 - bioD BNILDI_21025 0.84 -0.8 4,232,095 + -0.8 4,232,095 + -1.4 4,232,095 + -0.9 4,232,267 + bioC BNILDI_21030 0.27 +0.6 4,232,607 + bioC BNILDI_21030 0.72 -0.1 4,232,607 + bioC BNILDI_21030 0.72 -0.2 4,232,608 - bioC BNILDI_21030 0.72 -0.7 4,232,689 + bioC BNILDI_21030 0.83 -0.8 4,232,689 + bioC BNILDI_21030 0.83 -0.3 4,232,693 + bioC BNILDI_21030 0.84 -0.4 4,232,694 - bioC BNILDI_21030 0.84 +1.7 4,232,739 + bioC BNILDI_21030 0.90 +0.4 4,232,739 + bioC BNILDI_21030 0.90 -0.2 4,233,167 + bioF BNILDI_21035 0.32 -1.8 4,233,167 + bioF BNILDI_21035 0.32 -1.4 4,233,167 + bioF BNILDI_21035 0.32 -1.0 4,233,168 - bioF BNILDI_21035 0.32 -0.6 4,233,209 + bioF BNILDI_21035 0.35 -1.2 4,233,210 + bioF BNILDI_21035 0.35 -0.5 4,233,210 + bioF BNILDI_21035 0.35 -0.7 4,233,210 + bioF BNILDI_21035 0.35 +1.2 4,233,211 - bioF BNILDI_21035 0.35 -2.0 4,233,211 - bioF BNILDI_21035 0.35 -0.3 4,233,211 - bioF BNILDI_21035 0.35 -0.3 4,233,380 - bioF BNILDI_21035 0.50 -1.8 4,233,532 + bioF BNILDI_21035 0.63 -0.2 4,233,647 - bioF BNILDI_21035 0.73 -0.3 4,233,647 - bioF BNILDI_21035 0.73 -0.9 4,233,711 + bioF BNILDI_21035 0.79 +0.2 4,233,802 + bioF BNILDI_21035 0.86 -1.4 4,233,803 - bioF BNILDI_21035 0.87 -1.1 4,233,812 + bioF BNILDI_21035 0.87 -0.9 4,233,813 - bioF BNILDI_21035 0.87 -1.0 4,233,958 - +0.4 4,233,958 - -0.5 4,233,973 + +1.7 4,234,505 + bioB BNILDI_21040 0.53 -2.2 4,234,505 + bioB BNILDI_21040 0.53 -1.2 4,234,547 - bioB BNILDI_21040 0.57 -1.9 4,234,547 - bioB BNILDI_21040 0.57 -1.4 4,234,547 - bioB BNILDI_21040 0.57 -0.8 4,234,547 - bioB BNILDI_21040 0.57 +0.4 4,234,549 + bioB BNILDI_21040 0.57 -0.6 4,234,550 - bioB BNILDI_21040 0.57 -2.8 4,234,611 - bioB BNILDI_21040 0.63 -2.0
Or see this region's nucleotide sequence