Strain Fitness in Escherichia coli ECRC62 around BNILDI_20245

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcmoM and elyC are separated by 135 nucleotideselyC and ycbJ overlap by 24 nucleotidesycbJ and kdsB are separated by 153 nucleotideskdsB and ycaR overlap by 4 nucleotides BNILDI_20235: cmoM - tRNA uridine 5-oxyacetic acid(34) methyltransferase CmoM, at 4,067,352 to 4,068,137 cmoM BNILDI_20240: elyC - envelope biogenesis factor ElyC, at 4,068,273 to 4,069,052 elyC BNILDI_20245: ycbJ - Uncharacterized protein YcbJ, at 4,069,029 to 4,069,922 ycbJ BNILDI_20250: kdsB - 3-deoxy-manno-octulosonate cytidylyltransferase, at 4,070,076 to 4,070,822 kdsB BNILDI_20255: ycaR - protein YcaR, at 4,070,819 to 4,071,001 ycaR Position (kb) 4069 4070Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4068.054 kb on - strand, within cmoMat 4068.223 kb on - strandat 4068.223 kb on - strandat 4068.245 kb on + strandat 4068.245 kb on + strandat 4068.245 kb on + strandat 4068.245 kb on + strandat 4068.245 kb on + strandat 4068.246 kb on - strandat 4068.246 kb on - strandat 4068.246 kb on - strandat 4068.247 kb on + strandat 4068.248 kb on - strandat 4068.248 kb on - strandat 4068.334 kb on + strandat 4068.335 kb on - strandat 4068.338 kb on + strandat 4068.401 kb on + strand, within elyCat 4068.402 kb on - strand, within elyCat 4068.494 kb on + strand, within elyCat 4068.494 kb on + strand, within elyCat 4068.495 kb on - strand, within elyCat 4068.495 kb on - strand, within elyCat 4068.513 kb on + strand, within elyCat 4068.513 kb on + strand, within elyCat 4068.513 kb on + strand, within elyCat 4068.537 kb on + strand, within elyCat 4068.539 kb on + strand, within elyCat 4068.661 kb on + strand, within elyCat 4068.683 kb on + strand, within elyCat 4068.684 kb on - strand, within elyCat 4068.694 kb on + strand, within elyCat 4068.694 kb on + strand, within elyCat 4068.695 kb on - strand, within elyCat 4068.741 kb on - strand, within elyCat 4069.123 kb on - strand, within ycbJat 4069.129 kb on + strand, within ycbJat 4069.129 kb on + strand, within ycbJat 4069.129 kb on + strand, within ycbJat 4069.129 kb on + strand, within ycbJat 4069.129 kb on + strand, within ycbJat 4069.130 kb on - strand, within ycbJat 4069.130 kb on - strand, within ycbJat 4069.258 kb on - strand, within ycbJat 4069.368 kb on + strand, within ycbJat 4069.433 kb on + strand, within ycbJat 4069.459 kb on - strand, within ycbJat 4069.696 kb on + strand, within ycbJat 4069.696 kb on + strand, within ycbJat 4069.696 kb on + strand, within ycbJat 4069.697 kb on - strand, within ycbJat 4069.697 kb on - strand, within ycbJat 4069.700 kb on + strand, within ycbJat 4069.701 kb on - strand, within ycbJat 4069.779 kb on - strand, within ycbJat 4069.868 kb on + strandat 4069.869 kb on - strandat 4069.890 kb on + strandat 4069.968 kb on + strandat 4069.968 kb on + strandat 4069.968 kb on + strandat 4069.969 kb on - strandat 4069.971 kb on - strandat 4069.971 kb on - strandat 4070.032 kb on + strandat 4070.078 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,068,054 - cmoM BNILDI_20235 0.89 +0.3
4,068,223 - -1.3
4,068,223 - -2.3
4,068,245 + +1.3
4,068,245 + -0.2
4,068,245 + +0.2
4,068,245 + +1.5
4,068,245 + +0.2
4,068,246 - +0.1
4,068,246 - +0.4
4,068,246 - -0.8
4,068,247 + +0.5
4,068,248 - -1.2
4,068,248 - -0.4
4,068,334 + +0.2
4,068,335 - +0.7
4,068,338 + +0.4
4,068,401 + elyC BNILDI_20240 0.16 +0.7
4,068,402 - elyC BNILDI_20240 0.17 -0.1
4,068,494 + elyC BNILDI_20240 0.28 +0.4
4,068,494 + elyC BNILDI_20240 0.28 +0.1
4,068,495 - elyC BNILDI_20240 0.28 -2.4
4,068,495 - elyC BNILDI_20240 0.28 -0.3
4,068,513 + elyC BNILDI_20240 0.31 -3.5
4,068,513 + elyC BNILDI_20240 0.31 +0.9
4,068,513 + elyC BNILDI_20240 0.31 -0.6
4,068,537 + elyC BNILDI_20240 0.34 +0.1
4,068,539 + elyC BNILDI_20240 0.34 -2.8
4,068,661 + elyC BNILDI_20240 0.50 -0.1
4,068,683 + elyC BNILDI_20240 0.53 -1.2
4,068,684 - elyC BNILDI_20240 0.53 -2.6
4,068,694 + elyC BNILDI_20240 0.54 -0.5
4,068,694 + elyC BNILDI_20240 0.54 +0.4
4,068,695 - elyC BNILDI_20240 0.54 +0.4
4,068,741 - elyC BNILDI_20240 0.60 +2.2
4,069,123 - ycbJ BNILDI_20245 0.11 -0.6
4,069,129 + ycbJ BNILDI_20245 0.11 +0.4
4,069,129 + ycbJ BNILDI_20245 0.11 +1.2
4,069,129 + ycbJ BNILDI_20245 0.11 +1.1
4,069,129 + ycbJ BNILDI_20245 0.11 -0.4
4,069,129 + ycbJ BNILDI_20245 0.11 +1.0
4,069,130 - ycbJ BNILDI_20245 0.11 -0.2
4,069,130 - ycbJ BNILDI_20245 0.11 -1.0
4,069,258 - ycbJ BNILDI_20245 0.26 +0.2
4,069,368 + ycbJ BNILDI_20245 0.38 +0.3
4,069,433 + ycbJ BNILDI_20245 0.45 +1.0
4,069,459 - ycbJ BNILDI_20245 0.48 +1.6
4,069,696 + ycbJ BNILDI_20245 0.75 -3.2
4,069,696 + ycbJ BNILDI_20245 0.75 -1.4
4,069,696 + ycbJ BNILDI_20245 0.75 +0.5
4,069,697 - ycbJ BNILDI_20245 0.75 +0.1
4,069,697 - ycbJ BNILDI_20245 0.75 +0.4
4,069,700 + ycbJ BNILDI_20245 0.75 +0.6
4,069,701 - ycbJ BNILDI_20245 0.75 +1.0
4,069,779 - ycbJ BNILDI_20245 0.84 +0.3
4,069,868 + +1.6
4,069,869 - -4.1
4,069,890 + +2.2
4,069,968 + +1.2
4,069,968 + -0.1
4,069,968 + +0.2
4,069,969 - -1.5
4,069,971 - -0.1
4,069,971 - +0.0
4,070,032 + +1.3
4,070,078 - -1.0

Or see this region's nucleotide sequence