Strain Fitness in Escherichia coli ECRC62 around BNILDI_20195

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntompF_G141E and aspC are separated by 184 nucleotidesaspC and gloC are separated by 221 nucleotidesgloC and ycbK are separated by 26 nucleotides BNILDI_20190: ompF_G141E - outer membrane protein F OmpF, at 4,054,692 to 4,055,780 _G141E BNILDI_20195: aspC - aspartate transaminase, at 4,055,965 to 4,057,155 aspC BNILDI_20205: gloC - hydroxyacylglutathione hydrolase GloC, at 4,057,377 to 4,058,024 gloC BNILDI_20210: ycbK - Uncharacterized protein YcbK, at 4,058,051 to 4,058,599 ycbK Position (kb) 4055 4056 4057 4058Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4055.021 kb on + strand, within ompF_G141Eat 4055.022 kb on - strand, within ompF_G141Eat 4055.026 kb on - strand, within ompF_G141Eat 4055.061 kb on + strand, within ompF_G141Eat 4055.061 kb on + strand, within ompF_G141Eat 4055.062 kb on - strand, within ompF_G141Eat 4055.073 kb on + strand, within ompF_G141Eat 4055.089 kb on - strand, within ompF_G141Eat 4055.127 kb on + strand, within ompF_G141Eat 4055.169 kb on - strand, within ompF_G141Eat 4055.169 kb on - strand, within ompF_G141Eat 4055.172 kb on + strand, within ompF_G141Eat 4055.172 kb on + strand, within ompF_G141Eat 4055.173 kb on - strand, within ompF_G141Eat 4055.173 kb on - strand, within ompF_G141Eat 4055.234 kb on + strand, within ompF_G141Eat 4055.403 kb on - strand, within ompF_G141Eat 4055.416 kb on - strand, within ompF_G141Eat 4055.433 kb on + strand, within ompF_G141Eat 4055.433 kb on + strand, within ompF_G141Eat 4055.448 kb on + strand, within ompF_G141Eat 4055.448 kb on + strand, within ompF_G141Eat 4055.605 kb on + strand, within ompF_G141Eat 4055.606 kb on - strand, within ompF_G141Eat 4055.658 kb on + strand, within ompF_G141Eat 4055.659 kb on - strand, within ompF_G141Eat 4055.659 kb on - strand, within ompF_G141Eat 4055.661 kb on + strand, within ompF_G141Eat 4055.661 kb on + strand, within ompF_G141Eat 4055.685 kb on + strandat 4055.685 kb on + strandat 4055.686 kb on - strandat 4055.694 kb on + strandat 4055.694 kb on + strandat 4055.734 kb on + strandat 4055.779 kb on - strandat 4056.291 kb on - strand, within aspCat 4056.291 kb on - strand, within aspCat 4056.291 kb on - strand, within aspCat 4056.291 kb on - strand, within aspCat 4056.364 kb on - strand, within aspCat 4056.404 kb on - strand, within aspCat 4056.410 kb on - strand, within aspCat 4056.412 kb on + strand, within aspCat 4056.412 kb on + strand, within aspCat 4056.412 kb on + strand, within aspCat 4056.412 kb on + strand, within aspCat 4056.485 kb on + strand, within aspCat 4056.485 kb on + strand, within aspCat 4056.485 kb on + strand, within aspCat 4056.604 kb on + strand, within aspCat 4056.664 kb on - strand, within aspCat 4056.694 kb on + strand, within aspCat 4056.695 kb on - strand, within aspCat 4056.695 kb on - strand, within aspCat 4056.715 kb on + strand, within aspCat 4056.716 kb on - strand, within aspCat 4056.812 kb on - strand, within aspCat 4056.872 kb on + strand, within aspCat 4056.873 kb on - strand, within aspCat 4056.904 kb on - strand, within aspCat 4056.916 kb on - strand, within aspCat 4056.924 kb on + strand, within aspCat 4056.924 kb on + strand, within aspCat 4057.064 kb on + strandat 4057.064 kb on + strandat 4057.113 kb on + strandat 4057.242 kb on + strandat 4057.460 kb on + strand, within gloCat 4057.568 kb on + strand, within gloCat 4057.628 kb on + strand, within gloCat 4057.645 kb on - strand, within gloCat 4057.645 kb on - strand, within gloCat 4057.645 kb on - strand, within gloCat 4057.645 kb on - strand, within gloCat 4057.645 kb on - strand, within gloCat 4057.653 kb on + strand, within gloCat 4057.653 kb on + strand, within gloCat 4057.740 kb on - strand, within gloCat 4057.975 kb on - strandat 4058.017 kb on + strandat 4058.018 kb on - strandat 4058.018 kb on - strandat 4058.050 kb on - strandat 4058.052 kb on + strandat 4058.080 kb on + strandat 4058.092 kb on + strandat 4058.095 kb on + strandat 4058.096 kb on - strandat 4058.106 kb on + strand, within ycbKat 4058.107 kb on - strand, within ycbKat 4058.111 kb on + strand, within ycbKat 4058.129 kb on - strand, within ycbKat 4058.129 kb on - strand, within ycbKat 4058.149 kb on + strand, within ycbK

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,055,021 + ompF_G141E BNILDI_20190 0.30 +0.8
4,055,022 - ompF_G141E BNILDI_20190 0.30 +1.2
4,055,026 - ompF_G141E BNILDI_20190 0.31 +1.0
4,055,061 + ompF_G141E BNILDI_20190 0.34 +0.6
4,055,061 + ompF_G141E BNILDI_20190 0.34 +0.8
4,055,062 - ompF_G141E BNILDI_20190 0.34 -3.8
4,055,073 + ompF_G141E BNILDI_20190 0.35 +0.6
4,055,089 - ompF_G141E BNILDI_20190 0.36 -1.9
4,055,127 + ompF_G141E BNILDI_20190 0.40 -0.1
4,055,169 - ompF_G141E BNILDI_20190 0.44 +0.4
4,055,169 - ompF_G141E BNILDI_20190 0.44 +1.1
4,055,172 + ompF_G141E BNILDI_20190 0.44 +0.2
4,055,172 + ompF_G141E BNILDI_20190 0.44 -0.2
4,055,173 - ompF_G141E BNILDI_20190 0.44 -3.0
4,055,173 - ompF_G141E BNILDI_20190 0.44 -1.9
4,055,234 + ompF_G141E BNILDI_20190 0.50 +0.3
4,055,403 - ompF_G141E BNILDI_20190 0.65 -2.0
4,055,416 - ompF_G141E BNILDI_20190 0.66 -0.4
4,055,433 + ompF_G141E BNILDI_20190 0.68 +1.4
4,055,433 + ompF_G141E BNILDI_20190 0.68 -0.0
4,055,448 + ompF_G141E BNILDI_20190 0.69 -0.4
4,055,448 + ompF_G141E BNILDI_20190 0.69 +0.1
4,055,605 + ompF_G141E BNILDI_20190 0.84 +1.1
4,055,606 - ompF_G141E BNILDI_20190 0.84 +0.5
4,055,658 + ompF_G141E BNILDI_20190 0.89 -0.1
4,055,659 - ompF_G141E BNILDI_20190 0.89 +1.0
4,055,659 - ompF_G141E BNILDI_20190 0.89 +0.6
4,055,661 + ompF_G141E BNILDI_20190 0.89 +1.4
4,055,661 + ompF_G141E BNILDI_20190 0.89 +0.3
4,055,685 + -0.5
4,055,685 + +0.1
4,055,686 - +0.5
4,055,694 + -2.7
4,055,694 + +0.3
4,055,734 + +0.2
4,055,779 - -2.6
4,056,291 - aspC BNILDI_20195 0.27 -1.1
4,056,291 - aspC BNILDI_20195 0.27 -3.0
4,056,291 - aspC BNILDI_20195 0.27 +0.2
4,056,291 - aspC BNILDI_20195 0.27 -2.4
4,056,364 - aspC BNILDI_20195 0.34 +0.3
4,056,404 - aspC BNILDI_20195 0.37 -1.7
4,056,410 - aspC BNILDI_20195 0.37 +1.0
4,056,412 + aspC BNILDI_20195 0.38 -2.2
4,056,412 + aspC BNILDI_20195 0.38 +1.1
4,056,412 + aspC BNILDI_20195 0.38 -0.1
4,056,412 + aspC BNILDI_20195 0.38 -1.5
4,056,485 + aspC BNILDI_20195 0.44 -2.5
4,056,485 + aspC BNILDI_20195 0.44 -1.9
4,056,485 + aspC BNILDI_20195 0.44 -2.2
4,056,604 + aspC BNILDI_20195 0.54 -0.7
4,056,664 - aspC BNILDI_20195 0.59 -2.9
4,056,694 + aspC BNILDI_20195 0.61 -1.2
4,056,695 - aspC BNILDI_20195 0.61 -2.6
4,056,695 - aspC BNILDI_20195 0.61 -0.4
4,056,715 + aspC BNILDI_20195 0.63 -0.8
4,056,716 - aspC BNILDI_20195 0.63 -3.0
4,056,812 - aspC BNILDI_20195 0.71 -2.0
4,056,872 + aspC BNILDI_20195 0.76 -0.5
4,056,873 - aspC BNILDI_20195 0.76 -3.5
4,056,904 - aspC BNILDI_20195 0.79 -2.3
4,056,916 - aspC BNILDI_20195 0.80 -3.5
4,056,924 + aspC BNILDI_20195 0.81 -1.8
4,056,924 + aspC BNILDI_20195 0.81 -0.9
4,057,064 + -0.2
4,057,064 + -2.6
4,057,113 + -1.1
4,057,242 + -0.1
4,057,460 + gloC BNILDI_20205 0.13 -0.5
4,057,568 + gloC BNILDI_20205 0.29 -0.7
4,057,628 + gloC BNILDI_20205 0.39 +0.2
4,057,645 - gloC BNILDI_20205 0.41 -0.2
4,057,645 - gloC BNILDI_20205 0.41 +1.3
4,057,645 - gloC BNILDI_20205 0.41 +0.2
4,057,645 - gloC BNILDI_20205 0.41 -0.1
4,057,645 - gloC BNILDI_20205 0.41 +2.5
4,057,653 + gloC BNILDI_20205 0.43 +1.8
4,057,653 + gloC BNILDI_20205 0.43 -4.1
4,057,740 - gloC BNILDI_20205 0.56 +0.2
4,057,975 - -2.1
4,058,017 + +0.6
4,058,018 - -1.1
4,058,018 - -0.2
4,058,050 - +0.2
4,058,052 + +0.1
4,058,080 + +0.9
4,058,092 + -0.4
4,058,095 + -0.5
4,058,096 - -0.1
4,058,106 + ycbK BNILDI_20210 0.10 -0.9
4,058,107 - ycbK BNILDI_20210 0.10 +0.1
4,058,111 + ycbK BNILDI_20210 0.11 -2.5
4,058,129 - ycbK BNILDI_20210 0.14 -0.5
4,058,129 - ycbK BNILDI_20210 0.14 +0.2
4,058,149 + ycbK BNILDI_20210 0.18 +0.6

Or see this region's nucleotide sequence