Strain Fitness in Escherichia coli ECRC62 around BNILDI_20005

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrlmKL and ycbX are separated by 243 nucleotidesycbX and zapC overlap by 4 nucleotideszapC and pyrD are separated by 173 nucleotides BNILDI_20000: rlmKL - bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL, at 4,014,259 to 4,016,367 rlmKL BNILDI_20005: ycbX - 6-N-hydroxylaminopurine resistance protein YcbX, at 4,016,611 to 4,017,720 ycbX BNILDI_20010: zapC - cell division protein ZapC, at 4,017,717 to 4,018,259 zapC BNILDI_20015: pyrD - quinone-dependent dihydroorotate dehydrogenase, at 4,018,433 to 4,019,443 pyrD Position (kb) 4016 4017 4018Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4015.665 kb on + strand, within rlmKLat 4015.665 kb on + strand, within rlmKLat 4015.891 kb on + strand, within rlmKLat 4015.936 kb on - strand, within rlmKLat 4016.151 kb on - strand, within rlmKLat 4016.176 kb on - strandat 4016.196 kb on + strandat 4016.196 kb on + strandat 4016.197 kb on - strandat 4016.197 kb on - strandat 4016.384 kb on + strandat 4016.406 kb on + strandat 4016.406 kb on + strandat 4016.406 kb on + strandat 4016.464 kb on - strandat 4016.464 kb on - strandat 4016.510 kb on + strandat 4016.551 kb on + strandat 4016.551 kb on + strandat 4016.556 kb on - strandat 4016.558 kb on + strandat 4016.559 kb on - strandat 4016.588 kb on + strandat 4016.622 kb on - strandat 4016.670 kb on + strandat 4016.671 kb on - strandat 4016.739 kb on + strand, within ycbXat 4016.745 kb on + strand, within ycbXat 4016.746 kb on - strand, within ycbXat 4016.840 kb on - strand, within ycbXat 4016.840 kb on - strand, within ycbXat 4016.939 kb on + strand, within ycbXat 4016.970 kb on - strand, within ycbXat 4016.970 kb on - strand, within ycbXat 4017.108 kb on - strand, within ycbXat 4017.168 kb on + strand, within ycbXat 4017.482 kb on + strand, within ycbXat 4017.486 kb on + strand, within ycbXat 4017.486 kb on + strand, within ycbXat 4017.486 kb on + strand, within ycbXat 4017.520 kb on - strand, within ycbXat 4017.617 kb on + strandat 4017.681 kb on + strandat 4017.705 kb on + strandat 4017.705 kb on + strandat 4017.706 kb on - strandat 4017.706 kb on - strandat 4017.753 kb on - strandat 4017.843 kb on + strand, within zapCat 4017.850 kb on - strand, within zapCat 4017.850 kb on - strand, within zapCat 4018.050 kb on - strand, within zapCat 4018.053 kb on + strand, within zapCat 4018.174 kb on - strand, within zapCat 4018.179 kb on - strand, within zapCat 4018.179 kb on - strand, within zapCat 4018.179 kb on - strand, within zapCat 4018.226 kb on - strandat 4018.278 kb on - strandat 4018.279 kb on + strandat 4018.368 kb on + strandat 4018.457 kb on - strandat 4018.610 kb on - strand, within pyrD

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,015,665 + rlmKL BNILDI_20000 0.67 +1.9
4,015,665 + rlmKL BNILDI_20000 0.67 +0.3
4,015,891 + rlmKL BNILDI_20000 0.77 -2.2
4,015,936 - rlmKL BNILDI_20000 0.80 -1.0
4,016,151 - rlmKL BNILDI_20000 0.90 -1.1
4,016,176 - +0.2
4,016,196 + +0.2
4,016,196 + +0.0
4,016,197 - -0.3
4,016,197 - +0.2
4,016,384 + -3.2
4,016,406 + -1.7
4,016,406 + -2.6
4,016,406 + +1.4
4,016,464 - -0.6
4,016,464 - -4.4
4,016,510 + +0.1
4,016,551 + -0.2
4,016,551 + -0.7
4,016,556 - -2.9
4,016,558 + -0.1
4,016,559 - +0.5
4,016,588 + +0.7
4,016,622 - +1.7
4,016,670 + +0.3
4,016,671 - +1.4
4,016,739 + ycbX BNILDI_20005 0.12 -0.7
4,016,745 + ycbX BNILDI_20005 0.12 -0.8
4,016,746 - ycbX BNILDI_20005 0.12 -1.2
4,016,840 - ycbX BNILDI_20005 0.21 +0.3
4,016,840 - ycbX BNILDI_20005 0.21 -0.1
4,016,939 + ycbX BNILDI_20005 0.30 +0.0
4,016,970 - ycbX BNILDI_20005 0.32 -0.7
4,016,970 - ycbX BNILDI_20005 0.32 +0.8
4,017,108 - ycbX BNILDI_20005 0.45 -1.2
4,017,168 + ycbX BNILDI_20005 0.50 +0.3
4,017,482 + ycbX BNILDI_20005 0.78 +1.1
4,017,486 + ycbX BNILDI_20005 0.79 +0.5
4,017,486 + ycbX BNILDI_20005 0.79 -0.1
4,017,486 + ycbX BNILDI_20005 0.79 +0.7
4,017,520 - ycbX BNILDI_20005 0.82 +0.8
4,017,617 + +0.8
4,017,681 + +0.1
4,017,705 + -0.3
4,017,705 + +1.8
4,017,706 - -1.8
4,017,706 - +0.0
4,017,753 - +0.1
4,017,843 + zapC BNILDI_20010 0.23 +1.2
4,017,850 - zapC BNILDI_20010 0.24 -0.1
4,017,850 - zapC BNILDI_20010 0.24 -0.1
4,018,050 - zapC BNILDI_20010 0.61 +0.6
4,018,053 + zapC BNILDI_20010 0.62 -2.3
4,018,174 - zapC BNILDI_20010 0.84 +0.6
4,018,179 - zapC BNILDI_20010 0.85 +0.2
4,018,179 - zapC BNILDI_20010 0.85 +0.5
4,018,179 - zapC BNILDI_20010 0.85 +0.5
4,018,226 - -0.1
4,018,278 - -0.4
4,018,279 + -0.3
4,018,368 + +0.8
4,018,457 - -2.0
4,018,610 - pyrD BNILDI_20015 0.18 -2.8

Or see this region's nucleotide sequence