Strain Fitness in Escherichia coli ECRC62 around BNILDI_20000

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuup and rlmKL are separated by 11 nucleotidesrlmKL and ycbX are separated by 243 nucleotides BNILDI_19995: uup - ABC transporter ATP-binding protein, at 4,012,340 to 4,014,247 uup BNILDI_20000: rlmKL - bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL, at 4,014,259 to 4,016,367 rlmKL BNILDI_20005: ycbX - 6-N-hydroxylaminopurine resistance protein YcbX, at 4,016,611 to 4,017,720 ycbX Position (kb) 4014 4015 4016 4017Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 4013.527 kb on - strand, within uupat 4013.547 kb on + strand, within uupat 4013.804 kb on + strand, within uupat 4013.805 kb on - strand, within uupat 4013.938 kb on + strand, within uupat 4014.163 kb on + strandat 4014.248 kb on + strandat 4014.397 kb on - strandat 4014.651 kb on - strand, within rlmKLat 4014.651 kb on - strand, within rlmKLat 4014.654 kb on + strand, within rlmKLat 4014.850 kb on - strand, within rlmKLat 4014.984 kb on + strand, within rlmKLat 4015.037 kb on + strand, within rlmKLat 4015.037 kb on + strand, within rlmKLat 4015.084 kb on - strand, within rlmKLat 4015.085 kb on + strand, within rlmKLat 4015.085 kb on + strand, within rlmKLat 4015.281 kb on - strand, within rlmKLat 4015.281 kb on - strand, within rlmKLat 4015.313 kb on - strand, within rlmKLat 4015.313 kb on - strand, within rlmKLat 4015.605 kb on + strand, within rlmKLat 4015.606 kb on - strand, within rlmKLat 4015.606 kb on - strand, within rlmKLat 4015.606 kb on - strand, within rlmKLat 4015.606 kb on - strand, within rlmKLat 4015.606 kb on - strand, within rlmKLat 4015.665 kb on + strand, within rlmKLat 4015.665 kb on + strand, within rlmKLat 4015.891 kb on + strand, within rlmKLat 4015.936 kb on - strand, within rlmKLat 4016.151 kb on - strand, within rlmKLat 4016.176 kb on - strandat 4016.196 kb on + strandat 4016.196 kb on + strandat 4016.197 kb on - strandat 4016.197 kb on - strandat 4016.384 kb on + strandat 4016.406 kb on + strandat 4016.406 kb on + strandat 4016.406 kb on + strandat 4016.464 kb on - strandat 4016.464 kb on - strandat 4016.510 kb on + strandat 4016.551 kb on + strandat 4016.551 kb on + strandat 4016.556 kb on - strandat 4016.558 kb on + strandat 4016.559 kb on - strandat 4016.588 kb on + strandat 4016.622 kb on - strandat 4016.670 kb on + strandat 4016.671 kb on - strandat 4016.739 kb on + strand, within ycbXat 4016.745 kb on + strand, within ycbXat 4016.746 kb on - strand, within ycbXat 4016.840 kb on - strand, within ycbXat 4016.840 kb on - strand, within ycbXat 4016.939 kb on + strand, within ycbXat 4016.970 kb on - strand, within ycbXat 4016.970 kb on - strand, within ycbXat 4017.108 kb on - strand, within ycbXat 4017.168 kb on + strand, within ycbX

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,013,527 - uup BNILDI_19995 0.62 +0.6
4,013,547 + uup BNILDI_19995 0.63 -1.0
4,013,804 + uup BNILDI_19995 0.77 +0.1
4,013,805 - uup BNILDI_19995 0.77 -0.6
4,013,938 + uup BNILDI_19995 0.84 -0.1
4,014,163 + -0.3
4,014,248 + -0.8
4,014,397 - -1.1
4,014,651 - rlmKL BNILDI_20000 0.19 +1.2
4,014,651 - rlmKL BNILDI_20000 0.19 -3.2
4,014,654 + rlmKL BNILDI_20000 0.19 -0.5
4,014,850 - rlmKL BNILDI_20000 0.28 +1.0
4,014,984 + rlmKL BNILDI_20000 0.34 +0.4
4,015,037 + rlmKL BNILDI_20000 0.37 -2.0
4,015,037 + rlmKL BNILDI_20000 0.37 +1.1
4,015,084 - rlmKL BNILDI_20000 0.39 -3.5
4,015,085 + rlmKL BNILDI_20000 0.39 +1.6
4,015,085 + rlmKL BNILDI_20000 0.39 -1.1
4,015,281 - rlmKL BNILDI_20000 0.48 -3.8
4,015,281 - rlmKL BNILDI_20000 0.48 +1.1
4,015,313 - rlmKL BNILDI_20000 0.50 -0.3
4,015,313 - rlmKL BNILDI_20000 0.50 +2.2
4,015,605 + rlmKL BNILDI_20000 0.64 -1.6
4,015,606 - rlmKL BNILDI_20000 0.64 +3.6
4,015,606 - rlmKL BNILDI_20000 0.64 -1.9
4,015,606 - rlmKL BNILDI_20000 0.64 +0.3
4,015,606 - rlmKL BNILDI_20000 0.64 +0.0
4,015,606 - rlmKL BNILDI_20000 0.64 +0.2
4,015,665 + rlmKL BNILDI_20000 0.67 +1.9
4,015,665 + rlmKL BNILDI_20000 0.67 +0.3
4,015,891 + rlmKL BNILDI_20000 0.77 -2.2
4,015,936 - rlmKL BNILDI_20000 0.80 -1.0
4,016,151 - rlmKL BNILDI_20000 0.90 -1.1
4,016,176 - +0.2
4,016,196 + +0.2
4,016,196 + +0.0
4,016,197 - -0.3
4,016,197 - +0.2
4,016,384 + -3.2
4,016,406 + -1.7
4,016,406 + -2.6
4,016,406 + +1.4
4,016,464 - -0.6
4,016,464 - -4.4
4,016,510 + +0.1
4,016,551 + -0.2
4,016,551 + -0.7
4,016,556 - -2.9
4,016,558 + -0.1
4,016,559 - +0.5
4,016,588 + +0.7
4,016,622 - +1.7
4,016,670 + +0.3
4,016,671 - +1.4
4,016,739 + ycbX BNILDI_20005 0.12 -0.7
4,016,745 + ycbX BNILDI_20005 0.12 -0.8
4,016,746 - ycbX BNILDI_20005 0.12 -1.2
4,016,840 - ycbX BNILDI_20005 0.21 +0.3
4,016,840 - ycbX BNILDI_20005 0.21 -0.1
4,016,939 + ycbX BNILDI_20005 0.30 +0.0
4,016,970 - ycbX BNILDI_20005 0.32 -0.7
4,016,970 - ycbX BNILDI_20005 0.32 +0.8
4,017,108 - ycbX BNILDI_20005 0.45 -1.2
4,017,168 + ycbX BNILDI_20005 0.50 +0.3

Or see this region's nucleotide sequence