Strain Fitness in Escherichia coli ECRC62 around BNILDI_19800

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcspG and cspH are separated by 285 nucleotidescspH and gfcA are separated by 441 nucleotidesgfcA and gfcB are separated by 106 nucleotidesgfcB and gfcC overlap by 4 nucleotides BNILDI_19790: cspG - cold shock protein CspG, at 3,973,304 to 3,973,516 cspG BNILDI_19795: cspH - cold shock-like protein CspH, at 3,973,802 to 3,974,014 cspH BNILDI_19800: gfcA - Threonine-rich inner membrane protein GfcA, at 3,974,456 to 3,974,761 gfcA BNILDI_19805: gfcB - Uncharacterized lipoprotein GfcB, at 3,974,868 to 3,975,512 gfcB BNILDI_19810: gfcC - Uncharacterized protein GfcC, at 3,975,509 to 3,976,255 gfcC Position (kb) 3974 3975Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3973.575 kb on + strandat 3973.576 kb on - strandat 3973.619 kb on - strandat 3973.636 kb on + strandat 3974.121 kb on - strandat 3974.141 kb on - strandat 3974.144 kb on - strandat 3974.161 kb on - strandat 3974.171 kb on + strandat 3974.232 kb on - strandat 3974.279 kb on + strandat 3974.343 kb on - strandat 3974.345 kb on - strandat 3974.390 kb on + strandat 3974.421 kb on + strandat 3974.421 kb on + strandat 3974.425 kb on + strandat 3974.557 kb on + strand, within gfcAat 3974.560 kb on + strand, within gfcAat 3974.603 kb on - strand, within gfcAat 3974.605 kb on + strand, within gfcAat 3974.916 kb on - strandat 3975.025 kb on - strand, within gfcBat 3975.029 kb on - strand, within gfcBat 3975.072 kb on - strand, within gfcBat 3975.353 kb on + strand, within gfcBat 3975.528 kb on - strandat 3975.552 kb on + strandat 3975.593 kb on + strand, within gfcCat 3975.594 kb on - strand, within gfcCat 3975.594 kb on - strand, within gfcCat 3975.734 kb on - strand, within gfcCat 3975.740 kb on + strand, within gfcC

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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3,973,575 + -1.1
3,973,576 - -1.0
3,973,619 - -1.1
3,973,636 + +1.1
3,974,121 - +1.2
3,974,141 - +0.2
3,974,144 - -0.6
3,974,161 - +1.8
3,974,171 + -1.1
3,974,232 - +0.0
3,974,279 + -0.2
3,974,343 - +0.8
3,974,345 - +0.2
3,974,390 + +1.4
3,974,421 + -0.2
3,974,421 + -1.0
3,974,425 + -0.6
3,974,557 + gfcA BNILDI_19800 0.33 -0.0
3,974,560 + gfcA BNILDI_19800 0.34 -2.7
3,974,603 - gfcA BNILDI_19800 0.48 -3.0
3,974,605 + gfcA BNILDI_19800 0.49 -0.1
3,974,916 - -0.6
3,975,025 - gfcB BNILDI_19805 0.24 -1.2
3,975,029 - gfcB BNILDI_19805 0.25 -0.0
3,975,072 - gfcB BNILDI_19805 0.32 -0.2
3,975,353 + gfcB BNILDI_19805 0.75 -0.6
3,975,528 - -0.6
3,975,552 + -0.8
3,975,593 + gfcC BNILDI_19810 0.11 +0.3
3,975,594 - gfcC BNILDI_19810 0.11 +1.9
3,975,594 - gfcC BNILDI_19810 0.11 -0.3
3,975,734 - gfcC BNILDI_19810 0.30 +0.4
3,975,740 + gfcC BNILDI_19810 0.31 +0.4

Or see this region's nucleotide sequence