Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cspG and cspH are separated by 285 nucleotides cspH and gfcA are separated by 441 nucleotides gfcA and gfcB are separated by 106 nucleotides gfcB and gfcC overlap by 4 nucleotides
BNILDI_19790: cspG - cold shock protein CspG, at 3,973,304 to 3,973,516
cspG
BNILDI_19795: cspH - cold shock-like protein CspH, at 3,973,802 to 3,974,014
cspH
BNILDI_19800: gfcA - Threonine-rich inner membrane protein GfcA, at 3,974,456 to 3,974,761
gfcA
BNILDI_19805: gfcB - Uncharacterized lipoprotein GfcB, at 3,974,868 to 3,975,512
gfcB
BNILDI_19810: gfcC - Uncharacterized protein GfcC, at 3,975,509 to 3,976,255
gfcC
Position (kb)
3974
3975 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3973.575 kb on + strand at 3973.576 kb on - strand at 3973.619 kb on - strand at 3973.636 kb on + strand at 3974.121 kb on - strand at 3974.141 kb on - strand at 3974.144 kb on - strand at 3974.161 kb on - strand at 3974.171 kb on + strand at 3974.232 kb on - strand at 3974.279 kb on + strand at 3974.343 kb on - strand at 3974.345 kb on - strand at 3974.390 kb on + strand at 3974.421 kb on + strand at 3974.421 kb on + strand at 3974.425 kb on + strand at 3974.557 kb on + strand, within gfcA at 3974.560 kb on + strand, within gfcA at 3974.603 kb on - strand, within gfcA at 3974.605 kb on + strand, within gfcA at 3974.916 kb on - strand at 3975.025 kb on - strand, within gfcB at 3975.029 kb on - strand, within gfcB at 3975.072 kb on - strand, within gfcB at 3975.353 kb on + strand, within gfcB at 3975.528 kb on - strand at 3975.552 kb on + strand at 3975.593 kb on + strand, within gfcC at 3975.594 kb on - strand, within gfcC at 3975.594 kb on - strand, within gfcC at 3975.734 kb on - strand, within gfcC at 3975.740 kb on + strand, within gfcC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,973,575 + -1.1 3,973,576 - -1.0 3,973,619 - -1.1 3,973,636 + +1.1 3,974,121 - +1.2 3,974,141 - +0.2 3,974,144 - -0.6 3,974,161 - +1.8 3,974,171 + -1.1 3,974,232 - +0.0 3,974,279 + -0.2 3,974,343 - +0.8 3,974,345 - +0.2 3,974,390 + +1.4 3,974,421 + -0.2 3,974,421 + -1.0 3,974,425 + -0.6 3,974,557 + gfcA BNILDI_19800 0.33 -0.0 3,974,560 + gfcA BNILDI_19800 0.34 -2.7 3,974,603 - gfcA BNILDI_19800 0.48 -3.0 3,974,605 + gfcA BNILDI_19800 0.49 -0.1 3,974,916 - -0.6 3,975,025 - gfcB BNILDI_19805 0.24 -1.2 3,975,029 - gfcB BNILDI_19805 0.25 -0.0 3,975,072 - gfcB BNILDI_19805 0.32 -0.2 3,975,353 + gfcB BNILDI_19805 0.75 -0.6 3,975,528 - -0.6 3,975,552 + -0.8 3,975,593 + gfcC BNILDI_19810 0.11 +0.3 3,975,594 - gfcC BNILDI_19810 0.11 +1.9 3,975,594 - gfcC BNILDI_19810 0.11 -0.3 3,975,734 - gfcC BNILDI_19810 0.30 +0.4 3,975,740 + gfcC BNILDI_19810 0.31 +0.4
Or see this region's nucleotide sequence