Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BNILDI_19120 and ycfZ overlap by 4 nucleotides ycfZ and cobB are separated by 138 nucleotides cobB and nagK are separated by 15 nucleotides nagK and lolE are separated by 28 nucleotides
BNILDI_19120: BNILDI_19120 - DUF3592 domain-containing protein, at 3,843,763 to 3,844,224
_19120
BNILDI_19125: ycfZ - Inner membrane protein YcfZ, at 3,844,221 to 3,845,009
ycfZ
BNILDI_19130: cobB - NAD-dependent protein deacylase, at 3,845,148 to 3,845,969
cobB
BNILDI_19135: nagK - N-acetylglucosamine kinase, at 3,845,985 to 3,846,896
nagK
BNILDI_19140: lolE - lipoprotein-releasing ABC transporter permease subunit LolE, at 3,846,925 to 3,848,163
lolE
Position (kb)
3845
3846 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3844.169 kb on + strand, within BNILDI_19120 at 3844.252 kb on + strand at 3844.401 kb on - strand, within ycfZ at 3844.518 kb on - strand, within ycfZ at 3844.625 kb on - strand, within ycfZ at 3844.684 kb on + strand, within ycfZ at 3844.771 kb on + strand, within ycfZ at 3844.839 kb on - strand, within ycfZ at 3844.897 kb on + strand, within ycfZ at 3845.149 kb on + strand at 3845.150 kb on - strand at 3845.150 kb on - strand at 3845.152 kb on - strand at 3845.229 kb on - strand at 3845.367 kb on - strand, within cobB at 3845.369 kb on - strand, within cobB at 3845.454 kb on - strand, within cobB at 3845.454 kb on - strand, within cobB at 3845.534 kb on + strand, within cobB at 3845.577 kb on + strand, within cobB at 3845.578 kb on - strand, within cobB at 3845.578 kb on - strand, within cobB at 3845.627 kb on + strand, within cobB at 3845.695 kb on + strand, within cobB at 3845.696 kb on - strand, within cobB at 3845.696 kb on - strand, within cobB at 3845.796 kb on - strand, within cobB at 3845.877 kb on - strand, within cobB at 3845.969 kb on + strand at 3845.970 kb on - strand at 3845.970 kb on - strand at 3845.994 kb on + strand at 3846.000 kb on + strand at 3846.001 kb on - strand at 3846.001 kb on - strand at 3846.001 kb on - strand at 3846.075 kb on + strand at 3846.179 kb on + strand, within nagK at 3846.202 kb on - strand, within nagK at 3846.295 kb on + strand, within nagK at 3846.295 kb on + strand, within nagK at 3846.296 kb on - strand, within nagK at 3846.305 kb on - strand, within nagK at 3846.334 kb on + strand, within nagK at 3846.425 kb on + strand, within nagK at 3846.426 kb on - strand, within nagK at 3846.449 kb on - strand, within nagK at 3846.597 kb on + strand, within nagK at 3846.670 kb on + strand, within nagK at 3846.677 kb on + strand, within nagK at 3846.756 kb on + strand, within nagK at 3846.790 kb on + strand, within nagK at 3846.790 kb on + strand, within nagK at 3846.790 kb on + strand, within nagK at 3846.879 kb on - strand at 3846.892 kb on + strand at 3846.893 kb on - strand at 3846.893 kb on - strand at 3846.917 kb on + strand at 3846.918 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,844,169 + BNILDI_19120 0.88 +1.4 3,844,252 + -0.4 3,844,401 - ycfZ BNILDI_19125 0.23 +0.7 3,844,518 - ycfZ BNILDI_19125 0.38 +1.3 3,844,625 - ycfZ BNILDI_19125 0.51 +0.0 3,844,684 + ycfZ BNILDI_19125 0.59 -1.6 3,844,771 + ycfZ BNILDI_19125 0.70 -0.8 3,844,839 - ycfZ BNILDI_19125 0.78 +0.4 3,844,897 + ycfZ BNILDI_19125 0.86 +1.4 3,845,149 + -1.1 3,845,150 - -0.1 3,845,150 - -1.5 3,845,152 - -0.5 3,845,229 - -0.2 3,845,367 - cobB BNILDI_19130 0.27 +0.3 3,845,369 - cobB BNILDI_19130 0.27 -0.4 3,845,454 - cobB BNILDI_19130 0.37 -2.2 3,845,454 - cobB BNILDI_19130 0.37 -2.0 3,845,534 + cobB BNILDI_19130 0.47 +1.7 3,845,577 + cobB BNILDI_19130 0.52 +0.4 3,845,578 - cobB BNILDI_19130 0.52 +1.0 3,845,578 - cobB BNILDI_19130 0.52 -2.2 3,845,627 + cobB BNILDI_19130 0.58 -0.8 3,845,695 + cobB BNILDI_19130 0.67 -2.1 3,845,696 - cobB BNILDI_19130 0.67 +0.4 3,845,696 - cobB BNILDI_19130 0.67 -1.3 3,845,796 - cobB BNILDI_19130 0.79 -1.9 3,845,877 - cobB BNILDI_19130 0.89 -0.5 3,845,969 + +1.6 3,845,970 - +2.0 3,845,970 - -2.1 3,845,994 + +1.1 3,846,000 + +0.3 3,846,001 - +0.7 3,846,001 - -0.2 3,846,001 - -0.3 3,846,075 + -0.7 3,846,179 + nagK BNILDI_19135 0.21 +0.5 3,846,202 - nagK BNILDI_19135 0.24 -0.1 3,846,295 + nagK BNILDI_19135 0.34 +0.6 3,846,295 + nagK BNILDI_19135 0.34 +1.1 3,846,296 - nagK BNILDI_19135 0.34 +1.0 3,846,305 - nagK BNILDI_19135 0.35 +1.8 3,846,334 + nagK BNILDI_19135 0.38 +0.4 3,846,425 + nagK BNILDI_19135 0.48 -0.8 3,846,426 - nagK BNILDI_19135 0.48 -0.0 3,846,449 - nagK BNILDI_19135 0.51 +0.0 3,846,597 + nagK BNILDI_19135 0.67 -1.4 3,846,670 + nagK BNILDI_19135 0.75 +0.0 3,846,677 + nagK BNILDI_19135 0.76 -0.8 3,846,756 + nagK BNILDI_19135 0.85 -3.5 3,846,790 + nagK BNILDI_19135 0.88 +1.8 3,846,790 + nagK BNILDI_19135 0.88 +0.4 3,846,790 + nagK BNILDI_19135 0.88 +0.2 3,846,879 - +0.3 3,846,892 + -0.7 3,846,893 - -3.1 3,846,893 - +1.1 3,846,917 + +1.1 3,846,918 - +0.1
Or see this region's nucleotide sequence