Strain Fitness in Escherichia coli ECRC62 around BNILDI_19070

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmnmA and hflD are separated by 35 nucleotideshflD and purB are separated by 3 nucleotides BNILDI_19065: mnmA - tRNA 2-thiouridine(34) synthase MnmA, at 3,831,616 to 3,832,722 mnmA BNILDI_19070: hflD - high frequency lysogenization protein HflD, at 3,832,758 to 3,833,399 hflD BNILDI_19075: purB - adenylosuccinate lyase, at 3,833,403 to 3,834,773 purB Position (kb) 3832 3833 3834Strain fitness (log2 ratio) -3 -2 -1 0 1at 3832.724 kb on + strandat 3832.733 kb on + strandat 3832.733 kb on + strandat 3832.892 kb on + strand, within hflDat 3833.011 kb on + strand, within hflDat 3833.169 kb on + strand, within hflD

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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3,832,724 + +0.3
3,832,733 + -0.9
3,832,733 + -3.1
3,832,892 + hflD BNILDI_19070 0.21 -2.9
3,833,011 + hflD BNILDI_19070 0.39 -0.5
3,833,169 + hflD BNILDI_19070 0.64 -1.1

Or see this region's nucleotide sequence