Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt minC and minD are separated by 23 nucleotides minD and minE are separated by 3 nucleotides minE and BNILDI_18975 are separated by 371 nucleotides
BNILDI_18960: minC - septum site-determining protein MinC, at 3,813,178 to 3,813,873
minC
BNILDI_18965: minD - septum site-determining protein MinD, at 3,813,897 to 3,814,709
minD
BNILDI_18970: minE - cell division topological specificity factor MinE, at 3,814,713 to 3,814,979
minE
BNILDI_18975: BNILDI_18975 - autotransporter outer membrane beta-barrel domain-containing protein, at 3,815,351 to 3,815,677
_18975
Position (kb)
3814
3815 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3813.909 kb on - strand at 3814.127 kb on + strand, within minD at 3814.296 kb on + strand, within minD at 3814.296 kb on + strand, within minD at 3814.297 kb on - strand, within minD at 3814.847 kb on + strand, within minE at 3815.001 kb on - strand at 3815.072 kb on + strand at 3815.122 kb on + strand at 3815.154 kb on + strand at 3815.155 kb on - strand at 3815.522 kb on - strand, within BNILDI_18975 at 3815.643 kb on + strand, within BNILDI_18975 at 3815.687 kb on + strand at 3815.697 kb on + strand at 3815.713 kb on + strand at 3815.714 kb on - strand at 3815.714 kb on - strand at 3815.714 kb on - strand at 3815.783 kb on + strand at 3815.783 kb on + strand at 3815.875 kb on + strand at 3815.876 kb on - strand at 3815.905 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,813,909 - +0.1 3,814,127 + minD BNILDI_18965 0.28 -1.1 3,814,296 + minD BNILDI_18965 0.49 +1.4 3,814,296 + minD BNILDI_18965 0.49 -0.0 3,814,297 - minD BNILDI_18965 0.49 +0.1 3,814,847 + minE BNILDI_18970 0.50 -1.3 3,815,001 - -1.4 3,815,072 + +0.7 3,815,122 + +0.1 3,815,154 + -0.5 3,815,155 - +0.1 3,815,522 - BNILDI_18975 0.52 +0.3 3,815,643 + BNILDI_18975 0.89 +1.2 3,815,687 + +0.2 3,815,697 + -0.2 3,815,713 + -2.9 3,815,714 - -0.5 3,815,714 - +1.5 3,815,714 - +0.3 3,815,783 + +1.1 3,815,783 + +1.0 3,815,875 + -0.1 3,815,876 - -1.5 3,815,905 - +0.1
Or see this region's nucleotide sequence