Strain Fitness in Escherichia coli ECRC62 around BNILDI_18930

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntumuC and umuD overlap by 1 nucleotidesumuD and hlyE are separated by 372 nucleotideshlyE and ycgN are separated by 206 nucleotidesycgN and ycgM overlap by 32 nucleotides BNILDI_18915: umuC - DNA polymerase V catalytic protein, at 3,806,787 to 3,808,055 umuC BNILDI_18920: umuD - Protein UmuD, at 3,808,055 to 3,808,474 umuD BNILDI_18930: hlyE - hemolysin HlyE, at 3,808,847 to 3,809,758 hlyE BNILDI_18935: ycgN - YcgN family cysteine cluster protein, at 3,809,965 to 3,810,534 ycgN BNILDI_18940: ycgM - fumarylacetoacetate hydrolase family protein, at 3,810,503 to 3,811,162 ycgM Position (kb) 3808 3809 3810Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3808.025 kb on - strandat 3808.035 kb on + strandat 3808.036 kb on - strandat 3808.105 kb on + strand, within umuDat 3808.106 kb on - strand, within umuDat 3808.123 kb on + strand, within umuDat 3808.172 kb on + strand, within umuDat 3808.222 kb on + strand, within umuDat 3808.276 kb on + strand, within umuDat 3808.320 kb on + strand, within umuDat 3808.320 kb on + strand, within umuDat 3808.378 kb on - strand, within umuDat 3808.378 kb on - strand, within umuDat 3808.420 kb on + strand, within umuDat 3808.474 kb on + strandat 3808.474 kb on + strandat 3808.474 kb on + strandat 3808.474 kb on + strandat 3808.475 kb on - strandat 3808.475 kb on - strandat 3808.475 kb on - strandat 3808.557 kb on - strandat 3808.557 kb on - strandat 3808.727 kb on - strandat 3809.096 kb on + strand, within hlyEat 3809.156 kb on + strand, within hlyEat 3809.202 kb on + strand, within hlyEat 3809.378 kb on + strand, within hlyEat 3809.986 kb on - strandat 3809.995 kb on - strandat 3809.995 kb on - strandat 3810.089 kb on - strand, within ycgNat 3810.135 kb on - strand, within ycgNat 3810.224 kb on + strand, within ycgNat 3810.224 kb on + strand, within ycgNat 3810.246 kb on + strand, within ycgNat 3810.400 kb on + strand, within ycgNat 3810.400 kb on + strand, within ycgNat 3810.401 kb on - strand, within ycgNat 3810.433 kb on - strand, within ycgNat 3810.445 kb on - strand, within ycgNat 3810.504 kb on + strandat 3810.637 kb on + strand, within ycgMat 3810.638 kb on - strand, within ycgMat 3810.638 kb on - strand, within ycgMat 3810.638 kb on - strand, within ycgMat 3810.639 kb on + strand, within ycgMat 3810.639 kb on + strand, within ycgMat 3810.640 kb on - strand, within ycgMat 3810.682 kb on + strand, within ycgMat 3810.682 kb on + strand, within ycgMat 3810.728 kb on - strand, within ycgM

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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3,808,025 - -2.7
3,808,035 + -1.6
3,808,036 - +0.2
3,808,105 + umuD BNILDI_18920 0.12 +1.4
3,808,106 - umuD BNILDI_18920 0.12 +0.9
3,808,123 + umuD BNILDI_18920 0.16 +0.1
3,808,172 + umuD BNILDI_18920 0.28 -0.2
3,808,222 + umuD BNILDI_18920 0.40 +1.9
3,808,276 + umuD BNILDI_18920 0.53 +1.7
3,808,320 + umuD BNILDI_18920 0.63 -2.6
3,808,320 + umuD BNILDI_18920 0.63 +0.2
3,808,378 - umuD BNILDI_18920 0.77 +0.8
3,808,378 - umuD BNILDI_18920 0.77 +0.2
3,808,420 + umuD BNILDI_18920 0.87 -0.8
3,808,474 + +0.1
3,808,474 + +0.4
3,808,474 + -1.0
3,808,474 + +0.2
3,808,475 - -3.7
3,808,475 - +0.6
3,808,475 - -0.2
3,808,557 - +0.8
3,808,557 - +0.5
3,808,727 - +0.1
3,809,096 + hlyE BNILDI_18930 0.27 +3.1
3,809,156 + hlyE BNILDI_18930 0.34 +0.4
3,809,202 + hlyE BNILDI_18930 0.39 -0.0
3,809,378 + hlyE BNILDI_18930 0.58 +0.8
3,809,986 - +1.2
3,809,995 - -0.2
3,809,995 - -0.6
3,810,089 - ycgN BNILDI_18935 0.22 +0.5
3,810,135 - ycgN BNILDI_18935 0.30 +0.0
3,810,224 + ycgN BNILDI_18935 0.45 -2.5
3,810,224 + ycgN BNILDI_18935 0.45 +0.3
3,810,246 + ycgN BNILDI_18935 0.49 +0.2
3,810,400 + ycgN BNILDI_18935 0.76 -1.4
3,810,400 + ycgN BNILDI_18935 0.76 -0.3
3,810,401 - ycgN BNILDI_18935 0.76 +0.3
3,810,433 - ycgN BNILDI_18935 0.82 +0.6
3,810,445 - ycgN BNILDI_18935 0.84 -3.4
3,810,504 + -1.9
3,810,637 + ycgM BNILDI_18940 0.20 -1.6
3,810,638 - ycgM BNILDI_18940 0.20 +1.0
3,810,638 - ycgM BNILDI_18940 0.20 +0.5
3,810,638 - ycgM BNILDI_18940 0.20 -0.5
3,810,639 + ycgM BNILDI_18940 0.21 +0.0
3,810,639 + ycgM BNILDI_18940 0.21 +0.6
3,810,640 - ycgM BNILDI_18940 0.21 -0.3
3,810,682 + ycgM BNILDI_18940 0.27 +1.0
3,810,682 + ycgM BNILDI_18940 0.27 +2.6
3,810,728 - ycgM BNILDI_18940 0.34 -0.1

Or see this region's nucleotide sequence