Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt umuC and umuD overlap by 1 nucleotides umuD and hlyE are separated by 372 nucleotides hlyE and ycgN are separated by 206 nucleotides ycgN and ycgM overlap by 32 nucleotides
BNILDI_18915: umuC - DNA polymerase V catalytic protein, at 3,806,787 to 3,808,055
umuC
BNILDI_18920: umuD - Protein UmuD, at 3,808,055 to 3,808,474
umuD
BNILDI_18930: hlyE - hemolysin HlyE, at 3,808,847 to 3,809,758
hlyE
BNILDI_18935: ycgN - YcgN family cysteine cluster protein, at 3,809,965 to 3,810,534
ycgN
BNILDI_18940: ycgM - fumarylacetoacetate hydrolase family protein, at 3,810,503 to 3,811,162
ycgM
Position (kb)
3808
3809
3810 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3808.025 kb on - strand at 3808.035 kb on + strand at 3808.036 kb on - strand at 3808.105 kb on + strand, within umuD at 3808.106 kb on - strand, within umuD at 3808.123 kb on + strand, within umuD at 3808.172 kb on + strand, within umuD at 3808.222 kb on + strand, within umuD at 3808.276 kb on + strand, within umuD at 3808.320 kb on + strand, within umuD at 3808.320 kb on + strand, within umuD at 3808.378 kb on - strand, within umuD at 3808.378 kb on - strand, within umuD at 3808.420 kb on + strand, within umuD at 3808.474 kb on + strand at 3808.474 kb on + strand at 3808.474 kb on + strand at 3808.474 kb on + strand at 3808.475 kb on - strand at 3808.475 kb on - strand at 3808.475 kb on - strand at 3808.557 kb on - strand at 3808.557 kb on - strand at 3808.727 kb on - strand at 3809.096 kb on + strand, within hlyE at 3809.156 kb on + strand, within hlyE at 3809.202 kb on + strand, within hlyE at 3809.378 kb on + strand, within hlyE at 3809.986 kb on - strand at 3809.995 kb on - strand at 3809.995 kb on - strand at 3810.089 kb on - strand, within ycgN at 3810.135 kb on - strand, within ycgN at 3810.224 kb on + strand, within ycgN at 3810.224 kb on + strand, within ycgN at 3810.246 kb on + strand, within ycgN at 3810.400 kb on + strand, within ycgN at 3810.400 kb on + strand, within ycgN at 3810.401 kb on - strand, within ycgN at 3810.433 kb on - strand, within ycgN at 3810.445 kb on - strand, within ycgN at 3810.504 kb on + strand at 3810.637 kb on + strand, within ycgM at 3810.638 kb on - strand, within ycgM at 3810.638 kb on - strand, within ycgM at 3810.638 kb on - strand, within ycgM at 3810.639 kb on + strand, within ycgM at 3810.639 kb on + strand, within ycgM at 3810.640 kb on - strand, within ycgM at 3810.682 kb on + strand, within ycgM at 3810.682 kb on + strand, within ycgM at 3810.728 kb on - strand, within ycgM
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,808,025 - -2.7 3,808,035 + -1.6 3,808,036 - +0.2 3,808,105 + umuD BNILDI_18920 0.12 +1.4 3,808,106 - umuD BNILDI_18920 0.12 +0.9 3,808,123 + umuD BNILDI_18920 0.16 +0.1 3,808,172 + umuD BNILDI_18920 0.28 -0.2 3,808,222 + umuD BNILDI_18920 0.40 +1.9 3,808,276 + umuD BNILDI_18920 0.53 +1.7 3,808,320 + umuD BNILDI_18920 0.63 -2.6 3,808,320 + umuD BNILDI_18920 0.63 +0.2 3,808,378 - umuD BNILDI_18920 0.77 +0.8 3,808,378 - umuD BNILDI_18920 0.77 +0.2 3,808,420 + umuD BNILDI_18920 0.87 -0.8 3,808,474 + +0.1 3,808,474 + +0.4 3,808,474 + -1.0 3,808,474 + +0.2 3,808,475 - -3.7 3,808,475 - +0.6 3,808,475 - -0.2 3,808,557 - +0.8 3,808,557 - +0.5 3,808,727 - +0.1 3,809,096 + hlyE BNILDI_18930 0.27 +3.1 3,809,156 + hlyE BNILDI_18930 0.34 +0.4 3,809,202 + hlyE BNILDI_18930 0.39 -0.0 3,809,378 + hlyE BNILDI_18930 0.58 +0.8 3,809,986 - +1.2 3,809,995 - -0.2 3,809,995 - -0.6 3,810,089 - ycgN BNILDI_18935 0.22 +0.5 3,810,135 - ycgN BNILDI_18935 0.30 +0.0 3,810,224 + ycgN BNILDI_18935 0.45 -2.5 3,810,224 + ycgN BNILDI_18935 0.45 +0.3 3,810,246 + ycgN BNILDI_18935 0.49 +0.2 3,810,400 + ycgN BNILDI_18935 0.76 -1.4 3,810,400 + ycgN BNILDI_18935 0.76 -0.3 3,810,401 - ycgN BNILDI_18935 0.76 +0.3 3,810,433 - ycgN BNILDI_18935 0.82 +0.6 3,810,445 - ycgN BNILDI_18935 0.84 -3.4 3,810,504 + -1.9 3,810,637 + ycgM BNILDI_18940 0.20 -1.6 3,810,638 - ycgM BNILDI_18940 0.20 +1.0 3,810,638 - ycgM BNILDI_18940 0.20 +0.5 3,810,638 - ycgM BNILDI_18940 0.20 -0.5 3,810,639 + ycgM BNILDI_18940 0.21 +0.0 3,810,639 + ycgM BNILDI_18940 0.21 +0.6 3,810,640 - ycgM BNILDI_18940 0.21 -0.3 3,810,682 + ycgM BNILDI_18940 0.27 +1.0 3,810,682 + ycgM BNILDI_18940 0.27 +2.6 3,810,728 - ycgM BNILDI_18940 0.34 -0.1
Or see this region's nucleotide sequence