Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycgB and fadR are separated by 51 nucleotides fadR and nhaB are separated by 221 nucleotides nhaB and dsbB are separated by 145 nucleotides dsbB and umuC are separated by 45 nucleotides
BNILDI_18895: ycgB - SpoVR family protein, at 3,801,999 to 3,803,531
ycgB
BNILDI_18900: fadR - fatty acid metabolism transcriptional regulator FadR, at 3,803,583 to 3,804,302
fadR
BNILDI_18905: nhaB - Na(+)/H(+) antiporter NhaB, at 3,804,524 to 3,806,065
nhaB
BNILDI_18910: dsbB - disulfide bond formation protein DsbB, at 3,806,211 to 3,806,741
dsbB
BNILDI_18915: umuC - DNA polymerase V catalytic protein, at 3,806,787 to 3,808,055
umuC
Position (kb)
3804
3805
3806
3807 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3803.725 kb on + strand, within fadR at 3803.726 kb on - strand, within fadR at 3803.787 kb on - strand, within fadR at 3803.788 kb on + strand, within fadR at 3803.822 kb on - strand, within fadR at 3803.822 kb on - strand, within fadR at 3803.860 kb on - strand, within fadR at 3803.860 kb on - strand, within fadR at 3804.063 kb on + strand, within fadR at 3804.148 kb on - strand, within fadR at 3804.303 kb on - strand at 3804.362 kb on - strand at 3804.370 kb on - strand at 3804.483 kb on + strand at 3804.484 kb on - strand at 3804.499 kb on + strand at 3804.585 kb on - strand at 3804.748 kb on - strand, within nhaB at 3804.842 kb on + strand, within nhaB at 3804.842 kb on + strand, within nhaB at 3804.842 kb on + strand, within nhaB at 3804.843 kb on - strand, within nhaB at 3804.843 kb on - strand, within nhaB at 3804.870 kb on + strand, within nhaB at 3804.870 kb on + strand, within nhaB at 3805.012 kb on + strand, within nhaB at 3805.016 kb on + strand, within nhaB at 3805.066 kb on + strand, within nhaB at 3805.086 kb on - strand, within nhaB at 3805.758 kb on - strand, within nhaB at 3805.834 kb on + strand, within nhaB at 3805.935 kb on - strand at 3805.965 kb on - strand at 3805.965 kb on - strand at 3806.085 kb on + strand at 3806.085 kb on + strand at 3806.086 kb on - strand at 3806.086 kb on - strand at 3806.086 kb on - strand at 3806.086 kb on - strand at 3806.188 kb on - strand at 3806.193 kb on + strand at 3806.320 kb on + strand, within dsbB at 3806.343 kb on - strand, within dsbB at 3806.347 kb on - strand, within dsbB at 3806.421 kb on + strand, within dsbB at 3806.445 kb on + strand, within dsbB at 3806.446 kb on - strand, within dsbB at 3806.447 kb on + strand, within dsbB at 3806.448 kb on - strand, within dsbB at 3806.471 kb on - strand, within dsbB at 3806.471 kb on - strand, within dsbB at 3806.476 kb on - strand, within dsbB at 3806.668 kb on - strand, within dsbB at 3806.783 kb on - strand at 3806.783 kb on - strand at 3806.823 kb on - strand at 3806.824 kb on + strand at 3806.887 kb on + strand at 3806.922 kb on - strand, within umuC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,803,725 + fadR BNILDI_18900 0.20 +0.2 3,803,726 - fadR BNILDI_18900 0.20 -3.1 3,803,787 - fadR BNILDI_18900 0.28 -1.0 3,803,788 + fadR BNILDI_18900 0.28 -2.6 3,803,822 - fadR BNILDI_18900 0.33 -2.7 3,803,822 - fadR BNILDI_18900 0.33 -0.4 3,803,860 - fadR BNILDI_18900 0.38 -0.3 3,803,860 - fadR BNILDI_18900 0.38 -1.1 3,804,063 + fadR BNILDI_18900 0.67 -0.9 3,804,148 - fadR BNILDI_18900 0.78 -1.9 3,804,303 - -0.5 3,804,362 - -2.7 3,804,370 - +0.6 3,804,483 + +1.2 3,804,484 - +2.6 3,804,499 + -2.4 3,804,585 - -1.0 3,804,748 - nhaB BNILDI_18905 0.15 -1.0 3,804,842 + nhaB BNILDI_18905 0.21 +1.8 3,804,842 + nhaB BNILDI_18905 0.21 -2.7 3,804,842 + nhaB BNILDI_18905 0.21 +0.3 3,804,843 - nhaB BNILDI_18905 0.21 +1.1 3,804,843 - nhaB BNILDI_18905 0.21 -0.7 3,804,870 + nhaB BNILDI_18905 0.22 -2.9 3,804,870 + nhaB BNILDI_18905 0.22 -0.2 3,805,012 + nhaB BNILDI_18905 0.32 -3.0 3,805,016 + nhaB BNILDI_18905 0.32 -0.3 3,805,066 + nhaB BNILDI_18905 0.35 -0.5 3,805,086 - nhaB BNILDI_18905 0.36 +0.3 3,805,758 - nhaB BNILDI_18905 0.80 -2.0 3,805,834 + nhaB BNILDI_18905 0.85 +0.4 3,805,935 - +0.3 3,805,965 - -1.5 3,805,965 - -0.8 3,806,085 + -2.4 3,806,085 + -1.4 3,806,086 - -0.2 3,806,086 - +0.8 3,806,086 - -0.4 3,806,086 - -2.4 3,806,188 - -1.7 3,806,193 + +0.1 3,806,320 + dsbB BNILDI_18910 0.21 -1.2 3,806,343 - dsbB BNILDI_18910 0.25 -2.2 3,806,347 - dsbB BNILDI_18910 0.26 -0.9 3,806,421 + dsbB BNILDI_18910 0.40 +0.1 3,806,445 + dsbB BNILDI_18910 0.44 +1.8 3,806,446 - dsbB BNILDI_18910 0.44 -2.3 3,806,447 + dsbB BNILDI_18910 0.44 -3.0 3,806,448 - dsbB BNILDI_18910 0.45 -1.3 3,806,471 - dsbB BNILDI_18910 0.49 -2.7 3,806,471 - dsbB BNILDI_18910 0.49 -0.4 3,806,476 - dsbB BNILDI_18910 0.50 -3.7 3,806,668 - dsbB BNILDI_18910 0.86 -0.3 3,806,783 - -0.2 3,806,783 - -0.1 3,806,823 - +0.3 3,806,824 + -1.0 3,806,887 + -3.8 3,806,922 - umuC BNILDI_18915 0.11 -1.7
Or see this region's nucleotide sequence