Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tonB and BNILDI_18525 are separated by 112 nucleotides BNILDI_18525 and kch are separated by 298 nucleotides kch and clsA are separated by 370 nucleotides
BNILDI_18520: tonB - TonB system transport protein TonB, at 3,728,986 to 3,729,720
tonB
BNILDI_18525: BNILDI_18525 - YciI family protein, at 3,729,833 to 3,730,225
_18525
BNILDI_18530: kch - voltage-gated potassium channel protein, at 3,730,524 to 3,731,777
kch
BNILDI_18535: clsA - cardiolipin synthase ClsA, at 3,732,148 to 3,733,608
clsA
Position (kb)
3730
3731
3732 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3729.610 kb on + strand, within tonB at 3729.620 kb on + strand, within tonB at 3729.890 kb on - strand, within BNILDI_18525 at 3729.894 kb on + strand, within BNILDI_18525 at 3729.894 kb on + strand, within BNILDI_18525 at 3729.895 kb on - strand, within BNILDI_18525 at 3729.899 kb on + strand, within BNILDI_18525 at 3729.936 kb on - strand, within BNILDI_18525 at 3729.940 kb on + strand, within BNILDI_18525 at 3729.941 kb on - strand, within BNILDI_18525 at 3729.941 kb on - strand, within BNILDI_18525 at 3729.945 kb on - strand, within BNILDI_18525 at 3730.011 kb on + strand, within BNILDI_18525 at 3730.017 kb on + strand, within BNILDI_18525 at 3730.018 kb on - strand, within BNILDI_18525 at 3730.018 kb on - strand, within BNILDI_18525 at 3730.211 kb on - strand at 3730.228 kb on - strand at 3730.243 kb on - strand at 3730.330 kb on + strand at 3730.346 kb on + strand at 3730.347 kb on - strand at 3730.347 kb on - strand at 3730.347 kb on - strand at 3730.360 kb on - strand at 3730.360 kb on - strand at 3730.443 kb on - strand at 3730.464 kb on + strand at 3730.465 kb on - strand at 3730.495 kb on + strand at 3730.519 kb on + strand at 3730.539 kb on - strand at 3730.644 kb on - strand at 3730.724 kb on - strand, within kch at 3730.724 kb on - strand, within kch at 3730.724 kb on - strand, within kch at 3730.817 kb on - strand, within kch at 3730.980 kb on + strand, within kch at 3731.005 kb on + strand, within kch at 3731.053 kb on - strand, within kch at 3731.141 kb on + strand, within kch at 3731.142 kb on - strand, within kch at 3731.222 kb on - strand, within kch at 3731.237 kb on + strand, within kch at 3731.238 kb on - strand, within kch at 3731.593 kb on + strand, within kch at 3731.653 kb on + strand at 3731.673 kb on - strand at 3731.678 kb on + strand at 3731.679 kb on - strand at 3731.796 kb on + strand at 3731.802 kb on + strand at 3731.828 kb on - strand at 3731.830 kb on - strand at 3731.833 kb on + strand at 3731.833 kb on + strand at 3731.834 kb on - strand at 3731.865 kb on - strand at 3731.909 kb on + strand at 3731.909 kb on + strand at 3732.008 kb on + strand at 3732.009 kb on - strand at 3732.012 kb on + strand at 3732.013 kb on - strand at 3732.013 kb on - strand at 3732.018 kb on - strand at 3732.042 kb on - strand at 3732.116 kb on + strand at 3732.142 kb on + strand at 3732.148 kb on - strand at 3732.160 kb on + strand at 3732.162 kb on + strand at 3732.162 kb on + strand at 3732.412 kb on - strand, within clsA at 3732.418 kb on - strand, within clsA at 3732.577 kb on - strand, within clsA at 3732.579 kb on + strand, within clsA at 3732.595 kb on + strand, within clsA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,729,610 + tonB BNILDI_18520 0.85 +0.1 3,729,620 + tonB BNILDI_18520 0.86 -1.1 3,729,890 - BNILDI_18525 0.15 -0.1 3,729,894 + BNILDI_18525 0.16 +0.1 3,729,894 + BNILDI_18525 0.16 +0.8 3,729,895 - BNILDI_18525 0.16 -0.2 3,729,899 + BNILDI_18525 0.17 +0.3 3,729,936 - BNILDI_18525 0.26 +0.2 3,729,940 + BNILDI_18525 0.27 -0.2 3,729,941 - BNILDI_18525 0.27 -3.0 3,729,941 - BNILDI_18525 0.27 -0.7 3,729,945 - BNILDI_18525 0.28 +0.1 3,730,011 + BNILDI_18525 0.45 -0.1 3,730,017 + BNILDI_18525 0.47 -0.8 3,730,018 - BNILDI_18525 0.47 -0.1 3,730,018 - BNILDI_18525 0.47 -1.4 3,730,211 - +0.9 3,730,228 - -2.3 3,730,243 - +0.0 3,730,330 + +1.6 3,730,346 + +0.9 3,730,347 - +0.2 3,730,347 - +0.2 3,730,347 - +0.5 3,730,360 - +1.0 3,730,360 - -0.4 3,730,443 - +1.3 3,730,464 + +0.2 3,730,465 - -1.1 3,730,495 + +0.8 3,730,519 + +0.4 3,730,539 - +0.3 3,730,644 - +0.4 3,730,724 - kch BNILDI_18530 0.16 +0.4 3,730,724 - kch BNILDI_18530 0.16 +0.1 3,730,724 - kch BNILDI_18530 0.16 -0.3 3,730,817 - kch BNILDI_18530 0.23 -0.6 3,730,980 + kch BNILDI_18530 0.36 +0.5 3,731,005 + kch BNILDI_18530 0.38 -0.1 3,731,053 - kch BNILDI_18530 0.42 +2.7 3,731,141 + kch BNILDI_18530 0.49 +0.2 3,731,142 - kch BNILDI_18530 0.49 -2.4 3,731,222 - kch BNILDI_18530 0.56 -0.1 3,731,237 + kch BNILDI_18530 0.57 +0.5 3,731,238 - kch BNILDI_18530 0.57 +0.1 3,731,593 + kch BNILDI_18530 0.85 -0.2 3,731,653 + -0.1 3,731,673 - -1.5 3,731,678 + -0.3 3,731,679 - +0.3 3,731,796 + +1.6 3,731,802 + -0.1 3,731,828 - +0.3 3,731,830 - -1.9 3,731,833 + +0.1 3,731,833 + -1.5 3,731,834 - +0.1 3,731,865 - -0.2 3,731,909 + -3.6 3,731,909 + +0.7 3,732,008 + -0.1 3,732,009 - -1.2 3,732,012 + +0.0 3,732,013 - -3.6 3,732,013 - -0.8 3,732,018 - -0.2 3,732,042 - -0.4 3,732,116 + +0.5 3,732,142 + -1.7 3,732,148 - -1.0 3,732,160 + -2.3 3,732,162 + +0.2 3,732,162 + +0.4 3,732,412 - clsA BNILDI_18535 0.18 +0.2 3,732,418 - clsA BNILDI_18535 0.18 -0.1 3,732,577 - clsA BNILDI_18535 0.29 +4.4 3,732,579 + clsA BNILDI_18535 0.30 -2.3 3,732,595 + clsA BNILDI_18535 0.31 -0.1
Or see this region's nucleotide sequence