Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rluB and yciO are separated by 100 nucleotides yciO and yciV overlap by 4 nucleotides yciV and trpL are separated by 137 nucleotides trpL and BNILDI_18465 are separated by 91 nucleotides
BNILDI_18445: rluB - 23S rRNA pseudouridine(2605) synthase RluB, at 3,715,102 to 3,715,974
rluB
BNILDI_18450: yciO - L-threonylcarbamoyladenylate synthase, at 3,716,075 to 3,716,695
yciO
BNILDI_18455: yciV - 5'-3' exoribonuclease, at 3,716,692 to 3,717,573
yciV
BNILDI_18460: trpL - trp operon leader peptide, at 3,717,711 to 3,717,755
trpL
BNILDI_18465: BNILDI_18465 - hypothetical protein, at 3,717,847 to 3,721,005
_18465
Position (kb)
3716
3717
3718 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3715.757 kb on + strand, within rluB at 3715.758 kb on - strand, within rluB at 3715.905 kb on + strand at 3715.974 kb on + strand at 3715.975 kb on - strand at 3715.975 kb on - strand at 3715.975 kb on - strand at 3715.975 kb on - strand at 3716.033 kb on - strand at 3716.055 kb on + strand at 3716.055 kb on + strand at 3716.056 kb on - strand at 3716.059 kb on - strand at 3716.060 kb on + strand at 3716.076 kb on + strand at 3716.076 kb on + strand at 3716.077 kb on - strand at 3716.077 kb on - strand at 3716.078 kb on + strand at 3716.079 kb on - strand at 3716.100 kb on - strand at 3716.100 kb on - strand at 3716.100 kb on - strand at 3716.135 kb on - strand at 3716.193 kb on - strand, within yciO at 3716.193 kb on - strand, within yciO at 3716.321 kb on - strand, within yciO at 3716.405 kb on - strand, within yciO at 3716.405 kb on - strand, within yciO at 3716.406 kb on + strand, within yciO at 3716.406 kb on + strand, within yciO at 3716.406 kb on + strand, within yciO at 3716.407 kb on - strand, within yciO at 3716.407 kb on - strand, within yciO at 3716.407 kb on - strand, within yciO at 3716.407 kb on - strand, within yciO at 3716.463 kb on + strand, within yciO at 3716.464 kb on - strand, within yciO at 3716.501 kb on - strand, within yciO at 3716.539 kb on + strand, within yciO at 3716.540 kb on - strand, within yciO at 3716.558 kb on - strand, within yciO at 3716.621 kb on - strand, within yciO at 3716.695 kb on + strand at 3716.732 kb on - strand at 3716.830 kb on + strand, within yciV at 3716.830 kb on + strand, within yciV at 3716.830 kb on + strand, within yciV at 3716.831 kb on - strand, within yciV at 3716.831 kb on - strand, within yciV at 3716.956 kb on - strand, within yciV at 3717.024 kb on + strand, within yciV at 3717.205 kb on + strand, within yciV at 3717.206 kb on - strand, within yciV at 3717.235 kb on + strand, within yciV at 3717.300 kb on + strand, within yciV at 3717.309 kb on + strand, within yciV at 3717.528 kb on + strand at 3717.558 kb on - strand at 3717.558 kb on - strand at 3717.767 kb on + strand at 3717.768 kb on - strand at 3717.992 kb on + strand at 3718.094 kb on + strand at 3718.490 kb on + strand, within BNILDI_18465 at 3718.490 kb on + strand, within BNILDI_18465 at 3718.491 kb on - strand, within BNILDI_18465 at 3718.491 kb on - strand, within BNILDI_18465 at 3718.491 kb on - strand, within BNILDI_18465 at 3718.560 kb on + strand, within BNILDI_18465
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,715,757 + rluB BNILDI_18445 0.75 -1.9 3,715,758 - rluB BNILDI_18445 0.75 -1.4 3,715,905 + -1.3 3,715,974 + -1.0 3,715,975 - +0.5 3,715,975 - -1.3 3,715,975 - -3.3 3,715,975 - -0.7 3,716,033 - +1.6 3,716,055 + -1.9 3,716,055 + -0.4 3,716,056 - -0.6 3,716,059 - +1.3 3,716,060 + +0.6 3,716,076 + -2.0 3,716,076 + -0.1 3,716,077 - -1.3 3,716,077 - -0.2 3,716,078 + -1.5 3,716,079 - +2.8 3,716,100 - -0.0 3,716,100 - -0.3 3,716,100 - +0.4 3,716,135 - +0.7 3,716,193 - yciO BNILDI_18450 0.19 +0.4 3,716,193 - yciO BNILDI_18450 0.19 +0.2 3,716,321 - yciO BNILDI_18450 0.40 -1.6 3,716,405 - yciO BNILDI_18450 0.53 +0.3 3,716,405 - yciO BNILDI_18450 0.53 +0.3 3,716,406 + yciO BNILDI_18450 0.53 -1.4 3,716,406 + yciO BNILDI_18450 0.53 +1.7 3,716,406 + yciO BNILDI_18450 0.53 -3.0 3,716,407 - yciO BNILDI_18450 0.53 -1.4 3,716,407 - yciO BNILDI_18450 0.53 +0.5 3,716,407 - yciO BNILDI_18450 0.53 +0.3 3,716,407 - yciO BNILDI_18450 0.53 +0.8 3,716,463 + yciO BNILDI_18450 0.62 -0.6 3,716,464 - yciO BNILDI_18450 0.63 +0.9 3,716,501 - yciO BNILDI_18450 0.69 +1.0 3,716,539 + yciO BNILDI_18450 0.75 -0.7 3,716,540 - yciO BNILDI_18450 0.75 -0.0 3,716,558 - yciO BNILDI_18450 0.78 -0.5 3,716,621 - yciO BNILDI_18450 0.88 +1.6 3,716,695 + +0.9 3,716,732 - -0.9 3,716,830 + yciV BNILDI_18455 0.16 +0.4 3,716,830 + yciV BNILDI_18455 0.16 -4.5 3,716,830 + yciV BNILDI_18455 0.16 +1.3 3,716,831 - yciV BNILDI_18455 0.16 -0.2 3,716,831 - yciV BNILDI_18455 0.16 +0.6 3,716,956 - yciV BNILDI_18455 0.30 -0.1 3,717,024 + yciV BNILDI_18455 0.38 +0.6 3,717,205 + yciV BNILDI_18455 0.58 -2.0 3,717,206 - yciV BNILDI_18455 0.58 +0.8 3,717,235 + yciV BNILDI_18455 0.62 -3.0 3,717,300 + yciV BNILDI_18455 0.69 -1.9 3,717,309 + yciV BNILDI_18455 0.70 +0.2 3,717,528 + +1.6 3,717,558 - -1.9 3,717,558 - +2.9 3,717,767 + -0.1 3,717,768 - -1.7 3,717,992 + -1.0 3,718,094 + -5.0 3,718,490 + BNILDI_18465 0.20 -1.1 3,718,490 + BNILDI_18465 0.20 -3.2 3,718,491 - BNILDI_18465 0.20 -4.2 3,718,491 - BNILDI_18465 0.20 +1.1 3,718,491 - BNILDI_18465 0.20 -1.6 3,718,560 + BNILDI_18465 0.23 -1.8
Or see this region's nucleotide sequence