Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yciT and osmB are separated by 268 nucleotides osmB and yciH are separated by 125 nucleotides yciH and pyrF overlap by 1 nucleotides
BNILDI_18350: yciT - DNA-binding transcriptional regulator YciT, at 3,698,484 to 3,699,233
yciT
BNILDI_18355: osmB - osmotically-inducible lipoprotein OsmB, at 3,699,502 to 3,699,720
osmB
BNILDI_18360: yciH - stress response translation initiation inhibitor YciH, at 3,699,846 to 3,700,172
yciH
BNILDI_18365: pyrF - orotidine-5'-phosphate decarboxylase, at 3,700,172 to 3,700,969
pyrF
Position (kb)
3699
3700 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3698.504 kb on + strand at 3698.505 kb on - strand at 3698.509 kb on + strand at 3698.542 kb on + strand at 3698.679 kb on - strand, within yciT at 3698.691 kb on + strand, within yciT at 3698.692 kb on - strand, within yciT at 3698.694 kb on - strand, within yciT at 3698.823 kb on - strand, within yciT at 3698.841 kb on + strand, within yciT at 3698.841 kb on + strand, within yciT at 3698.841 kb on + strand, within yciT at 3698.841 kb on + strand, within yciT at 3698.898 kb on + strand, within yciT at 3698.956 kb on + strand, within yciT at 3698.956 kb on + strand, within yciT at 3699.109 kb on + strand, within yciT at 3699.110 kb on - strand, within yciT at 3699.118 kb on - strand, within yciT at 3699.204 kb on - strand at 3699.326 kb on + strand at 3699.390 kb on + strand at 3699.390 kb on + strand at 3699.444 kb on - strand at 3699.448 kb on - strand at 3699.452 kb on + strand at 3699.467 kb on + strand at 3699.498 kb on + strand at 3699.501 kb on + strand at 3699.586 kb on - strand, within osmB at 3699.671 kb on + strand, within osmB at 3699.672 kb on - strand, within osmB at 3699.942 kb on - strand, within yciH at 3700.099 kb on - strand, within yciH at 3700.125 kb on - strand, within yciH at 3700.187 kb on + strand at 3700.260 kb on + strand, within pyrF at 3700.260 kb on + strand, within pyrF at 3700.261 kb on - strand, within pyrF at 3700.261 kb on - strand, within pyrF at 3700.298 kb on - strand, within pyrF at 3700.298 kb on - strand, within pyrF at 3700.374 kb on - strand, within pyrF at 3700.595 kb on - strand, within pyrF at 3700.595 kb on - strand, within pyrF at 3700.638 kb on - strand, within pyrF
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,698,504 + -1.2 3,698,505 - +0.6 3,698,509 + -1.0 3,698,542 + +0.4 3,698,679 - yciT BNILDI_18350 0.26 -0.9 3,698,691 + yciT BNILDI_18350 0.28 -0.2 3,698,692 - yciT BNILDI_18350 0.28 +0.4 3,698,694 - yciT BNILDI_18350 0.28 -1.1 3,698,823 - yciT BNILDI_18350 0.45 -1.8 3,698,841 + yciT BNILDI_18350 0.48 -3.3 3,698,841 + yciT BNILDI_18350 0.48 +0.8 3,698,841 + yciT BNILDI_18350 0.48 +0.7 3,698,841 + yciT BNILDI_18350 0.48 +1.5 3,698,898 + yciT BNILDI_18350 0.55 -0.2 3,698,956 + yciT BNILDI_18350 0.63 -2.9 3,698,956 + yciT BNILDI_18350 0.63 +0.4 3,699,109 + yciT BNILDI_18350 0.83 +0.4 3,699,110 - yciT BNILDI_18350 0.83 -0.4 3,699,118 - yciT BNILDI_18350 0.85 +0.6 3,699,204 - -0.4 3,699,326 + -2.8 3,699,390 + -1.0 3,699,390 + +0.2 3,699,444 - +2.3 3,699,448 - -2.8 3,699,452 + -1.2 3,699,467 + -3.1 3,699,498 + +0.7 3,699,501 + +0.3 3,699,586 - osmB BNILDI_18355 0.38 -0.7 3,699,671 + osmB BNILDI_18355 0.77 -0.4 3,699,672 - osmB BNILDI_18355 0.78 +0.6 3,699,942 - yciH BNILDI_18360 0.29 +0.6 3,700,099 - yciH BNILDI_18360 0.77 -0.5 3,700,125 - yciH BNILDI_18360 0.85 +0.2 3,700,187 + -0.5 3,700,260 + pyrF BNILDI_18365 0.11 -3.7 3,700,260 + pyrF BNILDI_18365 0.11 -4.1 3,700,261 - pyrF BNILDI_18365 0.11 -4.9 3,700,261 - pyrF BNILDI_18365 0.11 -0.3 3,700,298 - pyrF BNILDI_18365 0.16 -3.7 3,700,298 - pyrF BNILDI_18365 0.16 -3.1 3,700,374 - pyrF BNILDI_18365 0.25 -5.2 3,700,595 - pyrF BNILDI_18365 0.53 -3.6 3,700,595 - pyrF BNILDI_18365 0.53 -1.7 3,700,638 - pyrF BNILDI_18365 0.58 -2.9
Or see this region's nucleotide sequence