Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydfI and ydfZ are separated by 35 nucleotides ydfZ and ydfH are separated by 177 nucleotides ydfH and ydfG are separated by 88 nucleotides
BNILDI_17115: ydfI - Uncharacterized oxidoreductase YdfI, at 3,429,957 to 3,431,417
ydfI
BNILDI_17120: ydfZ - Putative selenoprotein YdfZ, at 3,431,453 to 3,431,656
ydfZ
BNILDI_17125: ydfH - DNA-binding transcriptional regulator YdfH, at 3,431,834 to 3,432,520
ydfH
BNILDI_17130: ydfG - bifunctional NADP-dependent 3-hydroxy acid dehydrogenase/3-hydroxypropionate dehydrogenase YdfG, at 3,432,609 to 3,433,355
ydfG
Position (kb)
3431
3432 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3430.628 kb on + strand, within ydfI at 3430.628 kb on + strand, within ydfI at 3430.732 kb on + strand, within ydfI at 3430.732 kb on + strand, within ydfI at 3430.807 kb on - strand, within ydfI at 3431.016 kb on + strand, within ydfI at 3431.017 kb on - strand, within ydfI at 3431.327 kb on + strand at 3431.328 kb on - strand at 3431.359 kb on - strand at 3431.359 kb on - strand at 3431.454 kb on + strand at 3431.454 kb on + strand at 3431.455 kb on - strand at 3431.455 kb on - strand at 3431.455 kb on - strand at 3431.516 kb on + strand, within ydfZ at 3431.606 kb on - strand, within ydfZ at 3431.656 kb on + strand at 3431.656 kb on + strand at 3431.657 kb on - strand at 3431.827 kb on - strand at 3432.350 kb on - strand, within ydfH at 3432.353 kb on - strand, within ydfH at 3432.499 kb on + strand at 3432.500 kb on - strand at 3432.500 kb on - strand at 3432.558 kb on - strand at 3432.637 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,430,628 + ydfI BNILDI_17115 0.46 -1.1 3,430,628 + ydfI BNILDI_17115 0.46 -1.0 3,430,732 + ydfI BNILDI_17115 0.53 -2.9 3,430,732 + ydfI BNILDI_17115 0.53 +0.3 3,430,807 - ydfI BNILDI_17115 0.58 +1.4 3,431,016 + ydfI BNILDI_17115 0.72 +0.9 3,431,017 - ydfI BNILDI_17115 0.73 +0.1 3,431,327 + -0.4 3,431,328 - +0.4 3,431,359 - +0.1 3,431,359 - -2.6 3,431,454 + +0.1 3,431,454 + -1.4 3,431,455 - -0.7 3,431,455 - -1.0 3,431,455 - -0.4 3,431,516 + ydfZ BNILDI_17120 0.31 -0.4 3,431,606 - ydfZ BNILDI_17120 0.75 -2.1 3,431,656 + +2.0 3,431,656 + -1.1 3,431,657 - -0.2 3,431,827 - +0.1 3,432,350 - ydfH BNILDI_17125 0.75 -2.7 3,432,353 - ydfH BNILDI_17125 0.76 +0.0 3,432,499 + +0.9 3,432,500 - -0.4 3,432,500 - -2.3 3,432,558 - -0.2 3,432,637 + -1.7
Or see this region's nucleotide sequence