Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ldtE and lpp are separated by 63 nucleotides lpp and pykF are separated by 310 nucleotides pykF and fumD are separated by 556 nucleotides
BNILDI_16590: ldtE - L,D-transpeptidase LdtE, at 3,322,804 to 3,323,808
ldtE
BNILDI_16595: lpp - murein lipoprotein Lpp, at 3,323,872 to 3,324,108
lpp
BNILDI_16600: pykF - pyruvate kinase PykF, at 3,324,419 to 3,325,831
pykF
BNILDI_16605: fumD - fumarate hydratase FumD, at 3,326,388 to 3,326,597
fumD
Position (kb)
3324
3325
3326 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3323.430 kb on + strand, within ldtE at 3323.430 kb on + strand, within ldtE at 3323.430 kb on + strand, within ldtE at 3323.431 kb on - strand, within ldtE at 3323.431 kb on - strand, within ldtE at 3323.455 kb on - strand, within ldtE at 3323.545 kb on + strand, within ldtE at 3323.545 kb on + strand, within ldtE at 3323.635 kb on - strand, within ldtE at 3323.644 kb on - strand, within ldtE at 3323.680 kb on + strand, within ldtE at 3323.680 kb on + strand, within ldtE at 3323.681 kb on - strand, within ldtE at 3323.787 kb on + strand at 3323.787 kb on + strand at 3323.874 kb on - strand at 3323.874 kb on - strand at 3323.874 kb on - strand at 3323.874 kb on - strand at 3323.883 kb on - strand at 3323.887 kb on - strand at 3324.064 kb on - strand, within lpp at 3324.064 kb on - strand, within lpp at 3324.144 kb on - strand at 3324.149 kb on + strand at 3324.206 kb on + strand at 3324.207 kb on - strand at 3324.312 kb on + strand at 3324.313 kb on - strand at 3324.314 kb on + strand at 3324.417 kb on + strand at 3324.420 kb on + strand at 3324.664 kb on - strand, within pykF at 3324.668 kb on - strand, within pykF at 3324.671 kb on + strand, within pykF at 3324.878 kb on - strand, within pykF at 3325.030 kb on + strand, within pykF at 3325.064 kb on - strand, within pykF at 3325.406 kb on - strand, within pykF at 3325.504 kb on - strand, within pykF at 3325.544 kb on - strand, within pykF at 3325.769 kb on - strand at 3325.841 kb on - strand at 3325.941 kb on - strand at 3326.235 kb on + strand at 3326.258 kb on + strand at 3326.259 kb on - strand at 3326.262 kb on + strand at 3326.262 kb on + strand at 3326.262 kb on + strand at 3326.263 kb on - strand at 3326.263 kb on - strand at 3326.350 kb on + strand at 3326.350 kb on + strand at 3326.350 kb on + strand at 3326.350 kb on + strand at 3326.351 kb on - strand at 3326.352 kb on + strand at 3326.372 kb on + strand at 3326.382 kb on + strand at 3326.489 kb on + strand, within fumD at 3326.490 kb on - strand, within fumD at 3326.509 kb on + strand, within fumD at 3326.510 kb on - strand, within fumD at 3326.513 kb on + strand, within fumD
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,323,430 + ldtE BNILDI_16590 0.62 +0.9 3,323,430 + ldtE BNILDI_16590 0.62 -2.0 3,323,430 + ldtE BNILDI_16590 0.62 +0.5 3,323,431 - ldtE BNILDI_16590 0.62 +1.4 3,323,431 - ldtE BNILDI_16590 0.62 -0.3 3,323,455 - ldtE BNILDI_16590 0.65 +0.8 3,323,545 + ldtE BNILDI_16590 0.74 -0.3 3,323,545 + ldtE BNILDI_16590 0.74 +0.1 3,323,635 - ldtE BNILDI_16590 0.83 -0.4 3,323,644 - ldtE BNILDI_16590 0.84 -0.6 3,323,680 + ldtE BNILDI_16590 0.87 -2.9 3,323,680 + ldtE BNILDI_16590 0.87 +0.5 3,323,681 - ldtE BNILDI_16590 0.87 -1.6 3,323,787 + +1.0 3,323,787 + -0.5 3,323,874 - -0.0 3,323,874 - +0.2 3,323,874 - +0.1 3,323,874 - +1.1 3,323,883 - -0.1 3,323,887 - +1.3 3,324,064 - lpp BNILDI_16595 0.81 +0.4 3,324,064 - lpp BNILDI_16595 0.81 +1.1 3,324,144 - -0.3 3,324,149 + -0.0 3,324,206 + +0.9 3,324,207 - +0.1 3,324,312 + -0.4 3,324,313 - +0.2 3,324,314 + +0.6 3,324,417 + -3.5 3,324,420 + +2.3 3,324,664 - pykF BNILDI_16600 0.17 +0.1 3,324,668 - pykF BNILDI_16600 0.18 +1.8 3,324,671 + pykF BNILDI_16600 0.18 +0.3 3,324,878 - pykF BNILDI_16600 0.32 +0.9 3,325,030 + pykF BNILDI_16600 0.43 +1.3 3,325,064 - pykF BNILDI_16600 0.46 -0.4 3,325,406 - pykF BNILDI_16600 0.70 -1.7 3,325,504 - pykF BNILDI_16600 0.77 +0.3 3,325,544 - pykF BNILDI_16600 0.80 +0.5 3,325,769 - +1.1 3,325,841 - +0.2 3,325,941 - +0.3 3,326,235 + -0.1 3,326,258 + +0.4 3,326,259 - -1.7 3,326,262 + +0.8 3,326,262 + -0.2 3,326,262 + -1.2 3,326,263 - +1.1 3,326,263 - +0.9 3,326,350 + +0.5 3,326,350 + +0.6 3,326,350 + +0.3 3,326,350 + +0.3 3,326,351 - +0.2 3,326,352 + -0.1 3,326,372 + +0.3 3,326,382 + -0.6 3,326,489 + fumD BNILDI_16605 0.48 +0.4 3,326,490 - fumD BNILDI_16605 0.49 +0.0 3,326,509 + fumD BNILDI_16605 0.58 -1.3 3,326,510 - fumD BNILDI_16605 0.58 +1.2 3,326,513 + fumD BNILDI_16605 0.60 +0.3
Or see this region's nucleotide sequence