Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yniA and ydiZ are separated by 105 nucleotides ydiZ and pfkB are separated by 100 nucleotides pfkB and ydiY are separated by 286 nucleotides
BNILDI_16330: yniA - putative ketoamine kinase YniA, at 3,273,159 to 3,274,019
yniA
BNILDI_16335: ydiZ - Uncharacterized protein YdiZ, at 3,274,125 to 3,274,415
ydiZ
BNILDI_16340: pfkB - 6-phosphofructokinase II, at 3,274,516 to 3,275,445
pfkB
BNILDI_16345: ydiY - Uncharacterized protein YdiY, at 3,275,732 to 3,276,490
ydiY
Position (kb)
3274
3275
3276 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3273.766 kb on + strand, within yniA at 3273.819 kb on - strand, within yniA at 3273.828 kb on + strand, within yniA at 3273.829 kb on - strand, within yniA at 3273.957 kb on - strand at 3273.981 kb on + strand at 3274.220 kb on + strand, within ydiZ at 3274.248 kb on - strand, within ydiZ at 3274.262 kb on + strand, within ydiZ at 3274.346 kb on + strand, within ydiZ at 3274.415 kb on + strand at 3274.451 kb on + strand at 3274.451 kb on + strand at 3274.529 kb on + strand at 3274.696 kb on - strand, within pfkB at 3275.107 kb on + strand, within pfkB at 3275.162 kb on - strand, within pfkB at 3275.431 kb on - strand at 3275.436 kb on + strand at 3275.437 kb on - strand at 3275.437 kb on - strand at 3275.437 kb on - strand at 3275.456 kb on + strand at 3275.529 kb on - strand at 3275.615 kb on + strand at 3275.765 kb on - strand at 3275.765 kb on - strand at 3275.920 kb on + strand, within ydiY at 3275.920 kb on + strand, within ydiY at 3275.989 kb on + strand, within ydiY at 3275.989 kb on + strand, within ydiY at 3275.990 kb on - strand, within ydiY at 3275.990 kb on - strand, within ydiY at 3275.990 kb on - strand, within ydiY at 3275.998 kb on + strand, within ydiY at 3276.269 kb on + strand, within ydiY at 3276.371 kb on - strand, within ydiY
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,273,766 + yniA BNILDI_16330 0.70 +1.2 3,273,819 - yniA BNILDI_16330 0.77 +1.7 3,273,828 + yniA BNILDI_16330 0.78 -0.2 3,273,829 - yniA BNILDI_16330 0.78 -1.1 3,273,957 - +0.2 3,273,981 + -0.2 3,274,220 + ydiZ BNILDI_16335 0.33 +0.8 3,274,248 - ydiZ BNILDI_16335 0.42 -0.6 3,274,262 + ydiZ BNILDI_16335 0.47 -0.1 3,274,346 + ydiZ BNILDI_16335 0.76 -0.6 3,274,415 + -0.8 3,274,451 + +0.8 3,274,451 + -0.8 3,274,529 + -0.3 3,274,696 - pfkB BNILDI_16340 0.19 -2.7 3,275,107 + pfkB BNILDI_16340 0.64 -1.3 3,275,162 - pfkB BNILDI_16340 0.69 -0.2 3,275,431 - +0.7 3,275,436 + -2.3 3,275,437 - +3.5 3,275,437 - +1.0 3,275,437 - -0.4 3,275,456 + -1.2 3,275,529 - +1.1 3,275,615 + -0.2 3,275,765 - +0.4 3,275,765 - -2.3 3,275,920 + ydiY BNILDI_16345 0.25 +1.5 3,275,920 + ydiY BNILDI_16345 0.25 -2.4 3,275,989 + ydiY BNILDI_16345 0.34 -1.7 3,275,989 + ydiY BNILDI_16345 0.34 -0.7 3,275,990 - ydiY BNILDI_16345 0.34 -3.3 3,275,990 - ydiY BNILDI_16345 0.34 -1.3 3,275,990 - ydiY BNILDI_16345 0.34 -0.7 3,275,998 + ydiY BNILDI_16345 0.35 +0.3 3,276,269 + ydiY BNILDI_16345 0.71 -0.4 3,276,371 - ydiY BNILDI_16345 0.84 +0.3
Or see this region's nucleotide sequence