Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purT and yebG are separated by 133 nucleotides yebG and yebF are separated by 66 nucleotides yebF and BNILDI_15670 overlap by 87 nucleotides BNILDI_15670 and BNILDI_15675 are separated by 36 nucleotides BNILDI_15675 and yebE are separated by 59 nucleotides
BNILDI_15655: purT - formate-dependent phosphoribosylglycinamide formyltransferase, at 3,147,981 to 3,149,159
purT
BNILDI_15660: yebG - DNA damage-inducible protein YebG, at 3,149,293 to 3,149,583
yebG
BNILDI_15665: yebF - Protein YebF, at 3,149,650 to 3,150,006
yebF
BNILDI_15670: BNILDI_15670 - hypothetical protein, at 3,149,920 to 3,150,126
_15670
BNILDI_15675: BNILDI_15675 - hypothetical protein, at 3,150,163 to 3,150,273
_15675
BNILDI_15680: yebE - Inner membrane protein YebE, at 3,150,333 to 3,150,992
yebE
Position (kb)
3149
3150 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3148.498 kb on - strand, within purT at 3148.658 kb on + strand, within purT at 3148.905 kb on - strand, within purT at 3148.928 kb on - strand, within purT at 3148.928 kb on - strand, within purT at 3148.959 kb on + strand, within purT at 3148.960 kb on - strand, within purT at 3148.979 kb on + strand, within purT at 3148.982 kb on + strand, within purT at 3149.030 kb on - strand, within purT at 3149.109 kb on + strand at 3149.109 kb on + strand at 3149.110 kb on - strand at 3149.110 kb on - strand at 3149.110 kb on - strand at 3149.151 kb on + strand at 3149.151 kb on + strand at 3149.177 kb on + strand at 3149.311 kb on + strand at 3149.311 kb on + strand at 3149.311 kb on + strand at 3149.312 kb on - strand at 3149.374 kb on + strand, within yebG at 3149.374 kb on + strand, within yebG at 3149.379 kb on + strand, within yebG at 3149.610 kb on - strand at 3149.789 kb on + strand, within yebF at 3149.790 kb on - strand, within yebF at 3149.895 kb on + strand, within yebF at 3150.018 kb on - strand, within BNILDI_15670 at 3150.084 kb on - strand, within BNILDI_15670 at 3150.154 kb on + strand at 3150.188 kb on - strand, within BNILDI_15675 at 3150.188 kb on - strand, within BNILDI_15675 at 3150.192 kb on + strand, within BNILDI_15675 at 3150.192 kb on + strand, within BNILDI_15675 at 3150.193 kb on - strand, within BNILDI_15675 at 3150.193 kb on - strand, within BNILDI_15675 at 3150.197 kb on - strand, within BNILDI_15675 at 3150.197 kb on - strand, within BNILDI_15675 at 3150.201 kb on + strand, within BNILDI_15675 at 3150.244 kb on + strand, within BNILDI_15675 at 3150.270 kb on - strand at 3150.299 kb on + strand at 3150.347 kb on - strand at 3150.386 kb on + strand at 3150.440 kb on - strand, within yebE at 3150.471 kb on - strand, within yebE at 3150.471 kb on - strand, within yebE at 3150.482 kb on + strand, within yebE at 3150.492 kb on - strand, within yebE at 3150.498 kb on + strand, within yebE at 3150.561 kb on + strand, within yebE at 3150.561 kb on + strand, within yebE
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,148,498 - purT BNILDI_15655 0.44 -3.1 3,148,658 + purT BNILDI_15655 0.57 -0.7 3,148,905 - purT BNILDI_15655 0.78 -1.2 3,148,928 - purT BNILDI_15655 0.80 -0.4 3,148,928 - purT BNILDI_15655 0.80 -1.4 3,148,959 + purT BNILDI_15655 0.83 +0.0 3,148,960 - purT BNILDI_15655 0.83 -0.6 3,148,979 + purT BNILDI_15655 0.85 -1.2 3,148,982 + purT BNILDI_15655 0.85 +1.0 3,149,030 - purT BNILDI_15655 0.89 +0.8 3,149,109 + -0.3 3,149,109 + +0.1 3,149,110 - +0.8 3,149,110 - +1.0 3,149,110 - -2.1 3,149,151 + +0.6 3,149,151 + -0.2 3,149,177 + +0.4 3,149,311 + +0.6 3,149,311 + +0.9 3,149,311 + -0.2 3,149,312 - -0.0 3,149,374 + yebG BNILDI_15660 0.28 -0.6 3,149,374 + yebG BNILDI_15660 0.28 -2.6 3,149,379 + yebG BNILDI_15660 0.30 -1.1 3,149,610 - +0.1 3,149,789 + yebF BNILDI_15665 0.39 +0.8 3,149,790 - yebF BNILDI_15665 0.39 +0.1 3,149,895 + yebF BNILDI_15665 0.69 +0.8 3,150,018 - BNILDI_15670 0.47 -0.1 3,150,084 - BNILDI_15670 0.79 +1.2 3,150,154 + +0.1 3,150,188 - BNILDI_15675 0.23 -0.1 3,150,188 - BNILDI_15675 0.23 -0.2 3,150,192 + BNILDI_15675 0.26 +0.1 3,150,192 + BNILDI_15675 0.26 +1.1 3,150,193 - BNILDI_15675 0.27 -0.3 3,150,193 - BNILDI_15675 0.27 +0.2 3,150,197 - BNILDI_15675 0.31 -0.2 3,150,197 - BNILDI_15675 0.31 -0.2 3,150,201 + BNILDI_15675 0.34 -2.7 3,150,244 + BNILDI_15675 0.73 -0.6 3,150,270 - -0.5 3,150,299 + -0.6 3,150,347 - +0.2 3,150,386 + -1.8 3,150,440 - yebE BNILDI_15680 0.16 -0.3 3,150,471 - yebE BNILDI_15680 0.21 +1.8 3,150,471 - yebE BNILDI_15680 0.21 -3.2 3,150,482 + yebE BNILDI_15680 0.23 +0.4 3,150,492 - yebE BNILDI_15680 0.24 -0.2 3,150,498 + yebE BNILDI_15680 0.25 -0.7 3,150,561 + yebE BNILDI_15680 0.35 -1.4 3,150,561 + yebE BNILDI_15680 0.35 +0.4
Or see this region's nucleotide sequence