Strain Fitness in Escherichia coli ECRC62 around BNILDI_15660

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpurT and yebG are separated by 133 nucleotidesyebG and yebF are separated by 66 nucleotidesyebF and BNILDI_15670 overlap by 87 nucleotidesBNILDI_15670 and BNILDI_15675 are separated by 36 nucleotidesBNILDI_15675 and yebE are separated by 59 nucleotides BNILDI_15655: purT - formate-dependent phosphoribosylglycinamide formyltransferase, at 3,147,981 to 3,149,159 purT BNILDI_15660: yebG - DNA damage-inducible protein YebG, at 3,149,293 to 3,149,583 yebG BNILDI_15665: yebF - Protein YebF, at 3,149,650 to 3,150,006 yebF BNILDI_15670: BNILDI_15670 - hypothetical protein, at 3,149,920 to 3,150,126 _15670 BNILDI_15675: BNILDI_15675 - hypothetical protein, at 3,150,163 to 3,150,273 _15675 BNILDI_15680: yebE - Inner membrane protein YebE, at 3,150,333 to 3,150,992 yebE Position (kb) 3149 3150Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3148.498 kb on - strand, within purTat 3148.658 kb on + strand, within purTat 3148.905 kb on - strand, within purTat 3148.928 kb on - strand, within purTat 3148.928 kb on - strand, within purTat 3148.959 kb on + strand, within purTat 3148.960 kb on - strand, within purTat 3148.979 kb on + strand, within purTat 3148.982 kb on + strand, within purTat 3149.030 kb on - strand, within purTat 3149.109 kb on + strandat 3149.109 kb on + strandat 3149.110 kb on - strandat 3149.110 kb on - strandat 3149.110 kb on - strandat 3149.151 kb on + strandat 3149.151 kb on + strandat 3149.177 kb on + strandat 3149.311 kb on + strandat 3149.311 kb on + strandat 3149.311 kb on + strandat 3149.312 kb on - strandat 3149.374 kb on + strand, within yebGat 3149.374 kb on + strand, within yebGat 3149.379 kb on + strand, within yebGat 3149.610 kb on - strandat 3149.789 kb on + strand, within yebFat 3149.790 kb on - strand, within yebFat 3149.895 kb on + strand, within yebFat 3150.018 kb on - strand, within BNILDI_15670at 3150.084 kb on - strand, within BNILDI_15670at 3150.154 kb on + strandat 3150.188 kb on - strand, within BNILDI_15675at 3150.188 kb on - strand, within BNILDI_15675at 3150.192 kb on + strand, within BNILDI_15675at 3150.192 kb on + strand, within BNILDI_15675at 3150.193 kb on - strand, within BNILDI_15675at 3150.193 kb on - strand, within BNILDI_15675at 3150.197 kb on - strand, within BNILDI_15675at 3150.197 kb on - strand, within BNILDI_15675at 3150.201 kb on + strand, within BNILDI_15675at 3150.244 kb on + strand, within BNILDI_15675at 3150.270 kb on - strandat 3150.299 kb on + strandat 3150.347 kb on - strandat 3150.386 kb on + strandat 3150.440 kb on - strand, within yebEat 3150.471 kb on - strand, within yebEat 3150.471 kb on - strand, within yebEat 3150.482 kb on + strand, within yebEat 3150.492 kb on - strand, within yebEat 3150.498 kb on + strand, within yebEat 3150.561 kb on + strand, within yebEat 3150.561 kb on + strand, within yebE

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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3,148,498 - purT BNILDI_15655 0.44 -3.1
3,148,658 + purT BNILDI_15655 0.57 -0.7
3,148,905 - purT BNILDI_15655 0.78 -1.2
3,148,928 - purT BNILDI_15655 0.80 -0.4
3,148,928 - purT BNILDI_15655 0.80 -1.4
3,148,959 + purT BNILDI_15655 0.83 +0.0
3,148,960 - purT BNILDI_15655 0.83 -0.6
3,148,979 + purT BNILDI_15655 0.85 -1.2
3,148,982 + purT BNILDI_15655 0.85 +1.0
3,149,030 - purT BNILDI_15655 0.89 +0.8
3,149,109 + -0.3
3,149,109 + +0.1
3,149,110 - +0.8
3,149,110 - +1.0
3,149,110 - -2.1
3,149,151 + +0.6
3,149,151 + -0.2
3,149,177 + +0.4
3,149,311 + +0.6
3,149,311 + +0.9
3,149,311 + -0.2
3,149,312 - -0.0
3,149,374 + yebG BNILDI_15660 0.28 -0.6
3,149,374 + yebG BNILDI_15660 0.28 -2.6
3,149,379 + yebG BNILDI_15660 0.30 -1.1
3,149,610 - +0.1
3,149,789 + yebF BNILDI_15665 0.39 +0.8
3,149,790 - yebF BNILDI_15665 0.39 +0.1
3,149,895 + yebF BNILDI_15665 0.69 +0.8
3,150,018 - BNILDI_15670 0.47 -0.1
3,150,084 - BNILDI_15670 0.79 +1.2
3,150,154 + +0.1
3,150,188 - BNILDI_15675 0.23 -0.1
3,150,188 - BNILDI_15675 0.23 -0.2
3,150,192 + BNILDI_15675 0.26 +0.1
3,150,192 + BNILDI_15675 0.26 +1.1
3,150,193 - BNILDI_15675 0.27 -0.3
3,150,193 - BNILDI_15675 0.27 +0.2
3,150,197 - BNILDI_15675 0.31 -0.2
3,150,197 - BNILDI_15675 0.31 -0.2
3,150,201 + BNILDI_15675 0.34 -2.7
3,150,244 + BNILDI_15675 0.73 -0.6
3,150,270 - -0.5
3,150,299 + -0.6
3,150,347 - +0.2
3,150,386 + -1.8
3,150,440 - yebE BNILDI_15680 0.16 -0.3
3,150,471 - yebE BNILDI_15680 0.21 +1.8
3,150,471 - yebE BNILDI_15680 0.21 -3.2
3,150,482 + yebE BNILDI_15680 0.23 +0.4
3,150,492 - yebE BNILDI_15680 0.24 -0.2
3,150,498 + yebE BNILDI_15680 0.25 -0.7
3,150,561 + yebE BNILDI_15680 0.35 -1.4
3,150,561 + yebE BNILDI_15680 0.35 +0.4

Or see this region's nucleotide sequence