Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BNILDI_15490 and BNILDI_15495 overlap by 1 nucleotides BNILDI_15495 and ruvC overlap by 1 nucleotides ruvC and BNILDI_15505 are separated by 1 nucleotides BNILDI_15505 and yobI are separated by 70 nucleotides yobI and ruvA are separated by 138 nucleotides ruvA and ruvB are separated by 8 nucleotides
BNILDI_15490: BNILDI_15490 - putative transcriptional regulatory protein YebC, at 3,113,714 to 3,114,454
_15490
BNILDI_15495: BNILDI_15495 - Crossover junction endodeoxyribonuclease RuvC, at 3,114,454 to 3,114,489
_15495
BNILDI_15500: ruvC - crossover junction endodeoxyribonuclease RuvC, at 3,114,489 to 3,115,010
ruvC
BNILDI_15505: BNILDI_15505 - YebB family permuted papain-like enzyme, at 3,115,012 to 3,115,614
_15505
BNILDI_15510: yobI - Uncharacterized protein YobI, at 3,115,685 to 3,115,750
yobI
BNILDI_15515: ruvA - Holliday junction branch migration protein RuvA, at 3,115,889 to 3,116,500
ruvA
BNILDI_15520: ruvB - Holliday junction branch migration DNA helicase RuvB, at 3,116,509 to 3,117,519
ruvB
Position (kb)
3115
3116 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3114.102 kb on + strand, within BNILDI_15490 at 3114.107 kb on + strand, within BNILDI_15490 at 3114.207 kb on - strand, within BNILDI_15490 at 3114.253 kb on + strand, within BNILDI_15490 at 3114.823 kb on + strand, within ruvC at 3114.823 kb on + strand, within ruvC at 3115.009 kb on - strand at 3115.136 kb on - strand, within BNILDI_15505 at 3115.287 kb on + strand, within BNILDI_15505 at 3115.414 kb on - strand, within BNILDI_15505 at 3115.483 kb on - strand, within BNILDI_15505 at 3115.560 kb on + strand at 3115.560 kb on + strand at 3115.560 kb on + strand at 3115.606 kb on - strand at 3115.859 kb on + strand at 3115.859 kb on + strand at 3115.941 kb on + strand at 3116.077 kb on + strand, within ruvA at 3116.561 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,114,102 + BNILDI_15490 0.52 -1.0 3,114,107 + BNILDI_15490 0.53 +1.2 3,114,207 - BNILDI_15490 0.67 -0.9 3,114,253 + BNILDI_15490 0.73 +0.7 3,114,823 + ruvC BNILDI_15500 0.64 -2.0 3,114,823 + ruvC BNILDI_15500 0.64 -1.8 3,115,009 - -1.9 3,115,136 - BNILDI_15505 0.21 -1.5 3,115,287 + BNILDI_15505 0.46 -2.3 3,115,414 - BNILDI_15505 0.67 -2.2 3,115,483 - BNILDI_15505 0.78 -1.1 3,115,560 + -0.2 3,115,560 + +2.7 3,115,560 + +1.5 3,115,606 - -0.7 3,115,859 + -1.5 3,115,859 + -1.8 3,115,941 + +0.1 3,116,077 + ruvA BNILDI_15515 0.31 +0.0 3,116,561 + +1.5
Or see this region's nucleotide sequence