Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspS and nudB are separated by 60 nucleotides nudB and BNILDI_15490 are separated by 28 nucleotides BNILDI_15490 and BNILDI_15495 overlap by 1 nucleotides BNILDI_15495 and ruvC overlap by 1 nucleotides
BNILDI_15480: aspS - aspartate--tRNA ligase, at 3,111,343 to 3,113,115
aspS
BNILDI_15485: nudB - dihydroneopterin triphosphate diphosphatase, at 3,113,176 to 3,113,685
nudB
BNILDI_15490: BNILDI_15490 - putative transcriptional regulatory protein YebC, at 3,113,714 to 3,114,454
_15490
BNILDI_15495: BNILDI_15495 - Crossover junction endodeoxyribonuclease RuvC, at 3,114,454 to 3,114,489
_15495
BNILDI_15500: ruvC - crossover junction endodeoxyribonuclease RuvC, at 3,114,489 to 3,115,010
ruvC
Position (kb)
3113
3114 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3113.116 kb on + strand at 3113.127 kb on + strand at 3113.183 kb on + strand at 3113.183 kb on + strand at 3113.184 kb on - strand at 3113.187 kb on + strand at 3113.187 kb on + strand at 3113.191 kb on + strand at 3113.191 kb on + strand at 3113.191 kb on + strand at 3113.191 kb on + strand at 3113.192 kb on - strand at 3113.241 kb on + strand, within nudB at 3113.250 kb on + strand, within nudB at 3113.281 kb on + strand, within nudB at 3113.281 kb on + strand, within nudB at 3113.402 kb on + strand, within nudB at 3113.403 kb on - strand, within nudB at 3113.447 kb on + strand, within nudB at 3113.524 kb on - strand, within nudB at 3113.594 kb on - strand, within nudB at 3113.631 kb on + strand, within nudB at 3113.887 kb on + strand, within BNILDI_15490 at 3114.102 kb on + strand, within BNILDI_15490 at 3114.107 kb on + strand, within BNILDI_15490 at 3114.207 kb on - strand, within BNILDI_15490 at 3114.253 kb on + strand, within BNILDI_15490
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,113,116 + -0.9 3,113,127 + -1.5 3,113,183 + -0.1 3,113,183 + -2.3 3,113,184 - +0.1 3,113,187 + -1.0 3,113,187 + -0.8 3,113,191 + +0.0 3,113,191 + -1.3 3,113,191 + -0.8 3,113,191 + -0.4 3,113,192 - +0.1 3,113,241 + nudB BNILDI_15485 0.13 -3.0 3,113,250 + nudB BNILDI_15485 0.15 -1.1 3,113,281 + nudB BNILDI_15485 0.21 -1.2 3,113,281 + nudB BNILDI_15485 0.21 +0.9 3,113,402 + nudB BNILDI_15485 0.44 -2.1 3,113,403 - nudB BNILDI_15485 0.45 -2.5 3,113,447 + nudB BNILDI_15485 0.53 -3.3 3,113,524 - nudB BNILDI_15485 0.68 +0.5 3,113,594 - nudB BNILDI_15485 0.82 -2.6 3,113,631 + nudB BNILDI_15485 0.89 -2.4 3,113,887 + BNILDI_15490 0.23 +0.7 3,114,102 + BNILDI_15490 0.52 -1.0 3,114,107 + BNILDI_15490 0.53 +1.2 3,114,207 - BNILDI_15490 0.67 -0.9 3,114,253 + BNILDI_15490 0.73 +0.7
Or see this region's nucleotide sequence