Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yedP and yodD are separated by 297 nucleotides yodD and dsrB are separated by 162 nucleotides dsrB and rcsA are separated by 43 nucleotides rcsA and fliR are separated by 289 nucleotides
BNILDI_15050: yedP - mannosyl-3-phosphoglycerate phosphatase-related protein, at 3,038,370 to 3,039,185
yedP
BNILDI_15060: yodD - YodD family peroxide/acid resistance protein, at 3,039,483 to 3,039,710
yodD
BNILDI_15065: dsrB - protein DsrB, at 3,039,873 to 3,040,061
dsrB
BNILDI_15070: rcsA - transcriptional regulator RcsA, at 3,040,105 to 3,040,728
rcsA
BNILDI_15075: fliR - flagellar type III secretion system protein FliR, at 3,041,018 to 3,041,803
fliR
Position (kb)
3039
3040
3041 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3038.875 kb on - strand, within yedP at 3038.903 kb on + strand, within yedP at 3038.904 kb on - strand, within yedP at 3039.041 kb on + strand, within yedP at 3039.042 kb on - strand, within yedP at 3039.047 kb on + strand, within yedP at 3039.115 kb on + strand at 3039.115 kb on + strand at 3039.119 kb on + strand at 3039.119 kb on + strand at 3039.120 kb on - strand at 3039.120 kb on - strand at 3039.319 kb on - strand at 3039.431 kb on + strand at 3039.554 kb on + strand, within yodD at 3039.554 kb on + strand, within yodD at 3039.554 kb on + strand, within yodD at 3039.774 kb on + strand at 3039.774 kb on + strand at 3039.778 kb on + strand at 3039.781 kb on - strand at 3039.893 kb on - strand, within dsrB at 3039.969 kb on + strand, within dsrB at 3040.013 kb on + strand, within dsrB at 3040.060 kb on - strand at 3040.177 kb on - strand, within rcsA at 3040.606 kb on + strand, within rcsA at 3040.687 kb on + strand at 3040.705 kb on + strand at 3040.733 kb on + strand at 3040.774 kb on + strand at 3040.812 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,038,875 - yedP BNILDI_15050 0.62 -2.7 3,038,903 + yedP BNILDI_15050 0.65 -0.2 3,038,904 - yedP BNILDI_15050 0.65 -0.7 3,039,041 + yedP BNILDI_15050 0.82 +0.2 3,039,042 - yedP BNILDI_15050 0.82 -0.2 3,039,047 + yedP BNILDI_15050 0.83 +0.0 3,039,115 + +0.0 3,039,115 + -0.2 3,039,119 + +1.6 3,039,119 + -4.3 3,039,120 - +0.2 3,039,120 - +0.7 3,039,319 - +0.8 3,039,431 + +0.1 3,039,554 + yodD BNILDI_15060 0.31 +1.6 3,039,554 + yodD BNILDI_15060 0.31 -3.1 3,039,554 + yodD BNILDI_15060 0.31 -2.0 3,039,774 + -0.2 3,039,774 + +0.2 3,039,778 + +0.4 3,039,781 - +0.5 3,039,893 - dsrB BNILDI_15065 0.11 -1.8 3,039,969 + dsrB BNILDI_15065 0.51 -1.4 3,040,013 + dsrB BNILDI_15065 0.74 -0.4 3,040,060 - +0.5 3,040,177 - rcsA BNILDI_15070 0.12 -0.2 3,040,606 + rcsA BNILDI_15070 0.80 +2.3 3,040,687 + +1.1 3,040,705 + +0.5 3,040,733 + +0.6 3,040,774 + -1.2 3,040,812 - -2.4
Or see this region's nucleotide sequence