Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeeW and cbtA overlap by 4 nucleotides cbtA and cbtA are separated by 88 nucleotides cbtA and yeeT are separated by 73 nucleotides
BNILDI_14830: yeeW - Protein YeeW, at 2,992,773 to 2,992,967
yeeW
BNILDI_14835: cbtA - type IV toxin-antitoxin system toxin CbtA, at 2,992,964 to 2,993,338
cbtA
BNILDI_14840: cbtA - type IV toxin-antitoxin system toxin CbtA, at 2,993,427 to 2,993,795
cbtA
BNILDI_14845: yeeT - Uncharacterized protein YeeT, at 2,993,869 to 2,994,090
yeeT
Position (kb)
2992
2993
2994 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2991.996 kb on + strand at 2991.997 kb on - strand at 2991.997 kb on - strand at 2992.099 kb on + strand at 2992.100 kb on - strand at 2992.117 kb on + strand at 2992.135 kb on + strand at 2992.267 kb on - strand at 2992.267 kb on - strand at 2992.277 kb on + strand at 2992.352 kb on + strand at 2992.356 kb on + strand at 2992.357 kb on - strand at 2992.357 kb on - strand at 2992.357 kb on - strand at 2992.367 kb on - strand at 2992.386 kb on + strand at 2992.520 kb on + strand at 2992.525 kb on + strand at 2992.559 kb on + strand at 2992.562 kb on + strand at 2992.585 kb on - strand at 2992.709 kb on + strand at 2992.710 kb on - strand at 2992.710 kb on - strand at 2992.751 kb on + strand at 2992.751 kb on + strand at 2992.849 kb on + strand, within yeeW at 2992.936 kb on - strand, within yeeW at 2992.999 kb on + strand at 2993.000 kb on - strand at 2993.000 kb on - strand at 2993.013 kb on + strand, within cbtA at 2993.014 kb on - strand, within cbtA at 2993.014 kb on - strand, within cbtA at 2993.065 kb on + strand, within cbtA at 2993.072 kb on + strand, within cbtA at 2993.076 kb on - strand, within cbtA at 2993.239 kb on - strand, within cbtA at 2993.367 kb on - strand at 2993.518 kb on + strand, within cbtA at 2993.518 kb on + strand, within cbtA at 2993.977 kb on - strand, within yeeT at 2993.978 kb on + strand, within yeeT at 2993.978 kb on + strand, within yeeT at 2994.263 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,991,996 + -3.6 2,991,997 - -0.3 2,991,997 - +0.7 2,992,099 + -1.1 2,992,100 - -2.2 2,992,117 + -1.1 2,992,135 + +0.2 2,992,267 - +0.1 2,992,267 - -2.4 2,992,277 + +1.9 2,992,352 + -3.1 2,992,356 + -0.1 2,992,357 - -0.2 2,992,357 - -0.7 2,992,357 - +0.4 2,992,367 - +0.9 2,992,386 + -3.0 2,992,520 + +0.6 2,992,525 + -1.6 2,992,559 + +0.1 2,992,562 + +0.2 2,992,585 - -0.6 2,992,709 + -0.6 2,992,710 - +0.6 2,992,710 - +2.1 2,992,751 + +0.6 2,992,751 + -0.1 2,992,849 + yeeW BNILDI_14830 0.39 -0.2 2,992,936 - yeeW BNILDI_14830 0.84 +0.6 2,992,999 + +0.3 2,993,000 - +0.1 2,993,000 - +1.4 2,993,013 + cbtA BNILDI_14835 0.13 -0.2 2,993,014 - cbtA BNILDI_14835 0.13 +0.1 2,993,014 - cbtA BNILDI_14835 0.13 +0.1 2,993,065 + cbtA BNILDI_14835 0.27 -0.3 2,993,072 + cbtA BNILDI_14835 0.29 +1.0 2,993,076 - cbtA BNILDI_14835 0.30 +0.8 2,993,239 - cbtA BNILDI_14835 0.73 +0.1 2,993,367 - -0.1 2,993,518 + cbtA BNILDI_14840 0.25 -2.8 2,993,518 + cbtA BNILDI_14840 0.25 +0.1 2,993,977 - yeeT BNILDI_14845 0.49 -1.1 2,993,978 + yeeT BNILDI_14845 0.49 +1.1 2,993,978 + yeeT BNILDI_14845 0.49 -0.4 2,994,263 + +0.2
Or see this region's nucleotide sequence