Strain Fitness in Escherichia coli ECRC62 around BNILDI_13580

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntglpB and glpA overlap by 11 nucleotidesglpA and glpT are separated by 272 nucleotides BNILDI_13575: glpB - glycerol-3-phosphate dehydrogenase subunit GlpB, at 2,721,059 to 2,722,318 glpB BNILDI_13580: glpA - anaerobic glycerol-3-phosphate dehydrogenase subunit A, at 2,722,308 to 2,723,936 glpA BNILDI_13585: glpT - glycerol-3-phosphate transporter, at 2,724,209 to 2,725,567 glpT Position (kb) 2722 2723 2724Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2721.311 kb on + strand, within glpBat 2721.707 kb on + strand, within glpBat 2721.707 kb on + strand, within glpBat 2721.708 kb on - strand, within glpBat 2721.806 kb on + strand, within glpBat 2721.807 kb on - strand, within glpBat 2721.896 kb on + strand, within glpBat 2721.897 kb on - strand, within glpBat 2721.902 kb on + strand, within glpBat 2721.903 kb on - strand, within glpBat 2721.903 kb on - strand, within glpBat 2721.903 kb on - strand, within glpBat 2721.903 kb on - strand, within glpBat 2721.947 kb on + strand, within glpBat 2722.092 kb on + strand, within glpBat 2722.574 kb on + strand, within glpAat 2722.577 kb on + strand, within glpAat 2722.639 kb on - strand, within glpAat 2723.069 kb on + strand, within glpAat 2723.070 kb on - strand, within glpAat 2723.124 kb on + strand, within glpAat 2723.125 kb on - strand, within glpAat 2723.367 kb on - strand, within glpAat 2723.370 kb on + strand, within glpAat 2723.371 kb on - strand, within glpAat 2723.574 kb on + strand, within glpAat 2723.574 kb on + strand, within glpAat 2723.575 kb on - strand, within glpAat 2723.658 kb on - strand, within glpAat 2723.900 kb on + strandat 2723.969 kb on + strandat 2723.976 kb on - strandat 2723.978 kb on + strandat 2723.978 kb on + strandat 2724.182 kb on + strandat 2724.182 kb on + strandat 2724.276 kb on - strandat 2724.309 kb on + strandat 2724.361 kb on + strand, within glpTat 2724.369 kb on - strand, within glpTat 2724.582 kb on + strand, within glpTat 2724.583 kb on - strand, within glpTat 2724.637 kb on + strand, within glpTat 2724.640 kb on + strand, within glpTat 2724.641 kb on - strand, within glpTat 2724.641 kb on - strand, within glpTat 2724.641 kb on - strand, within glpTat 2724.641 kb on - strand, within glpTat 2724.641 kb on - strand, within glpTat 2724.779 kb on + strand, within glpTat 2724.780 kb on - strand, within glpTat 2724.780 kb on - strand, within glpTat 2724.781 kb on + strand, within glpTat 2724.813 kb on + strand, within glpTat 2724.899 kb on + strand, within glpT

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,721,311 + glpB BNILDI_13575 0.20 +0.1
2,721,707 + glpB BNILDI_13575 0.51 -1.7
2,721,707 + glpB BNILDI_13575 0.51 -3.1
2,721,708 - glpB BNILDI_13575 0.52 -1.6
2,721,806 + glpB BNILDI_13575 0.59 -0.6
2,721,807 - glpB BNILDI_13575 0.59 -1.0
2,721,896 + glpB BNILDI_13575 0.66 +0.2
2,721,897 - glpB BNILDI_13575 0.67 +1.6
2,721,902 + glpB BNILDI_13575 0.67 +1.9
2,721,903 - glpB BNILDI_13575 0.67 +0.1
2,721,903 - glpB BNILDI_13575 0.67 +0.7
2,721,903 - glpB BNILDI_13575 0.67 -0.8
2,721,903 - glpB BNILDI_13575 0.67 +0.8
2,721,947 + glpB BNILDI_13575 0.70 +2.1
2,722,092 + glpB BNILDI_13575 0.82 +0.1
2,722,574 + glpA BNILDI_13580 0.16 -3.1
2,722,577 + glpA BNILDI_13580 0.17 -1.0
2,722,639 - glpA BNILDI_13580 0.20 -1.6
2,723,069 + glpA BNILDI_13580 0.47 -0.4
2,723,070 - glpA BNILDI_13580 0.47 -0.2
2,723,124 + glpA BNILDI_13580 0.50 -2.2
2,723,125 - glpA BNILDI_13580 0.50 +0.2
2,723,367 - glpA BNILDI_13580 0.65 -0.2
2,723,370 + glpA BNILDI_13580 0.65 -0.3
2,723,371 - glpA BNILDI_13580 0.65 +1.2
2,723,574 + glpA BNILDI_13580 0.78 +0.8
2,723,574 + glpA BNILDI_13580 0.78 -1.1
2,723,575 - glpA BNILDI_13580 0.78 -0.0
2,723,658 - glpA BNILDI_13580 0.83 -0.4
2,723,900 + -0.0
2,723,969 + +0.3
2,723,976 - -1.8
2,723,978 + -1.7
2,723,978 + -0.8
2,724,182 + -0.5
2,724,182 + -0.4
2,724,276 - +0.8
2,724,309 + +0.9
2,724,361 + glpT BNILDI_13585 0.11 -2.3
2,724,369 - glpT BNILDI_13585 0.12 +0.2
2,724,582 + glpT BNILDI_13585 0.27 +0.1
2,724,583 - glpT BNILDI_13585 0.28 +0.1
2,724,637 + glpT BNILDI_13585 0.31 -1.1
2,724,640 + glpT BNILDI_13585 0.32 +0.8
2,724,641 - glpT BNILDI_13585 0.32 +0.6
2,724,641 - glpT BNILDI_13585 0.32 +0.2
2,724,641 - glpT BNILDI_13585 0.32 +0.1
2,724,641 - glpT BNILDI_13585 0.32 +0.5
2,724,641 - glpT BNILDI_13585 0.32 +2.3
2,724,779 + glpT BNILDI_13585 0.42 +0.3
2,724,780 - glpT BNILDI_13585 0.42 -0.2
2,724,780 - glpT BNILDI_13585 0.42 -1.6
2,724,781 + glpT BNILDI_13585 0.42 -0.4
2,724,813 + glpT BNILDI_13585 0.44 +0.2
2,724,899 + glpT BNILDI_13585 0.51 +2.2

Or see this region's nucleotide sequence