Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoF and nuoG are separated by 52 nucleotides nuoG and nuoH overlap by 4 nucleotides nuoH and nuoI are separated by 14 nucleotides
BNILDI_13400: nuoF - NADH-quinone oxidoreductase subunit NuoF, at 2,683,510 to 2,684,847
nuoF
BNILDI_13405: nuoG - NADH-quinone oxidoreductase subunit NuoG, at 2,684,900 to 2,687,626
nuoG
BNILDI_13410: nuoH - NADH-quinone oxidoreductase subunit NuoH, at 2,687,623 to 2,688,600
nuoH
BNILDI_13415: nuoI - NADH-quinone oxidoreductase subunit NuoI, at 2,688,615 to 2,689,157
nuoI
Position (kb)
2684
2685
2686
2687
2688 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2683.916 kb on - strand, within nuoF at 2684.040 kb on + strand, within nuoF at 2684.178 kb on + strand, within nuoF at 2684.179 kb on - strand, within nuoF at 2684.235 kb on + strand, within nuoF at 2684.899 kb on - strand at 2684.986 kb on + strand at 2685.101 kb on - strand at 2685.128 kb on - strand at 2685.626 kb on + strand, within nuoG at 2685.669 kb on + strand, within nuoG at 2685.677 kb on + strand, within nuoG at 2685.911 kb on + strand, within nuoG at 2685.912 kb on - strand, within nuoG at 2685.983 kb on + strand, within nuoG at 2685.983 kb on + strand, within nuoG at 2685.984 kb on - strand, within nuoG at 2685.985 kb on + strand, within nuoG at 2686.013 kb on + strand, within nuoG at 2686.014 kb on - strand, within nuoG at 2686.270 kb on - strand, within nuoG at 2686.419 kb on + strand, within nuoG at 2686.483 kb on + strand, within nuoG at 2686.800 kb on - strand, within nuoG at 2686.811 kb on + strand, within nuoG at 2687.346 kb on - strand, within nuoG at 2687.415 kb on - strand at 2687.415 kb on - strand at 2687.480 kb on - strand at 2687.483 kb on + strand at 2687.770 kb on - strand, within nuoH at 2687.895 kb on + strand, within nuoH at 2687.896 kb on - strand, within nuoH at 2688.346 kb on + strand, within nuoH at 2688.457 kb on - strand, within nuoH at 2688.513 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,683,916 - nuoF BNILDI_13400 0.30 -1.7 2,684,040 + nuoF BNILDI_13400 0.40 -2.6 2,684,178 + nuoF BNILDI_13400 0.50 -2.2 2,684,179 - nuoF BNILDI_13400 0.50 -2.6 2,684,235 + nuoF BNILDI_13400 0.54 -0.8 2,684,899 - -2.8 2,684,986 + -3.1 2,685,101 - -3.9 2,685,128 - -2.6 2,685,626 + nuoG BNILDI_13405 0.27 -0.3 2,685,669 + nuoG BNILDI_13405 0.28 -0.3 2,685,677 + nuoG BNILDI_13405 0.28 -1.9 2,685,911 + nuoG BNILDI_13405 0.37 -1.3 2,685,912 - nuoG BNILDI_13405 0.37 -2.5 2,685,983 + nuoG BNILDI_13405 0.40 -5.4 2,685,983 + nuoG BNILDI_13405 0.40 -0.0 2,685,984 - nuoG BNILDI_13405 0.40 -1.3 2,685,985 + nuoG BNILDI_13405 0.40 -1.6 2,686,013 + nuoG BNILDI_13405 0.41 -0.1 2,686,014 - nuoG BNILDI_13405 0.41 -0.8 2,686,270 - nuoG BNILDI_13405 0.50 -3.1 2,686,419 + nuoG BNILDI_13405 0.56 -1.3 2,686,483 + nuoG BNILDI_13405 0.58 -3.3 2,686,800 - nuoG BNILDI_13405 0.70 -1.1 2,686,811 + nuoG BNILDI_13405 0.70 -6.2 2,687,346 - nuoG BNILDI_13405 0.90 -3.7 2,687,415 - -3.1 2,687,415 - -0.8 2,687,480 - -2.6 2,687,483 + -3.8 2,687,770 - nuoH BNILDI_13410 0.15 -2.4 2,687,895 + nuoH BNILDI_13410 0.28 -1.7 2,687,896 - nuoH BNILDI_13410 0.28 -1.7 2,688,346 + nuoH BNILDI_13410 0.74 -1.8 2,688,457 - nuoH BNILDI_13410 0.85 -1.3 2,688,513 - -3.2
Or see this region's nucleotide sequence