Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutE and eutJ are separated by 10 nucleotides eutJ and eutG overlap by 11 nucleotides
BNILDI_12535: eutE - Acetaldehyde dehydrogenase (acetylating) EutE, at 2,515,157 to 2,516,560
eutE
BNILDI_12540: eutJ - ethanolamine utilization protein EutJ, at 2,516,571 to 2,517,407
eutJ
BNILDI_12545: eutG - ethanolamine utilization ethanol dehydrogenase EutG, at 2,517,397 to 2,518,584
eutG
Position (kb)
2516
2517
2518 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2515.581 kb on + strand, within eutE at 2515.581 kb on + strand, within eutE at 2515.585 kb on + strand, within eutE at 2515.726 kb on + strand, within eutE at 2515.977 kb on + strand, within eutE at 2515.994 kb on - strand, within eutE at 2516.387 kb on - strand, within eutE at 2516.532 kb on + strand at 2516.535 kb on + strand at 2516.993 kb on - strand, within eutJ at 2516.993 kb on - strand, within eutJ at 2516.993 kb on - strand, within eutJ at 2516.993 kb on - strand, within eutJ at 2517.114 kb on - strand, within eutJ at 2517.114 kb on - strand, within eutJ at 2517.138 kb on + strand, within eutJ at 2517.139 kb on - strand, within eutJ at 2517.244 kb on + strand, within eutJ at 2517.244 kb on + strand, within eutJ at 2517.266 kb on + strand, within eutJ at 2517.267 kb on - strand, within eutJ at 2517.325 kb on + strand at 2517.368 kb on + strand at 2517.394 kb on + strand at 2517.394 kb on + strand at 2517.394 kb on + strand at 2517.395 kb on - strand at 2517.395 kb on - strand at 2517.395 kb on - strand at 2517.647 kb on + strand, within eutG at 2518.057 kb on + strand, within eutG at 2518.058 kb on - strand, within eutG at 2518.058 kb on - strand, within eutG at 2518.075 kb on - strand, within eutG at 2518.148 kb on - strand, within eutG at 2518.195 kb on - strand, within eutG at 2518.195 kb on - strand, within eutG at 2518.273 kb on - strand, within eutG
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,515,581 + eutE BNILDI_12535 0.30 +0.2 2,515,581 + eutE BNILDI_12535 0.30 -3.0 2,515,585 + eutE BNILDI_12535 0.30 -0.2 2,515,726 + eutE BNILDI_12535 0.41 +0.1 2,515,977 + eutE BNILDI_12535 0.58 +1.2 2,515,994 - eutE BNILDI_12535 0.60 +1.2 2,516,387 - eutE BNILDI_12535 0.88 +0.4 2,516,532 + -2.7 2,516,535 + -0.2 2,516,993 - eutJ BNILDI_12540 0.50 -4.5 2,516,993 - eutJ BNILDI_12540 0.50 -2.3 2,516,993 - eutJ BNILDI_12540 0.50 +1.0 2,516,993 - eutJ BNILDI_12540 0.50 +0.5 2,517,114 - eutJ BNILDI_12540 0.65 +0.2 2,517,114 - eutJ BNILDI_12540 0.65 +0.9 2,517,138 + eutJ BNILDI_12540 0.68 -0.3 2,517,139 - eutJ BNILDI_12540 0.68 -1.0 2,517,244 + eutJ BNILDI_12540 0.80 +1.1 2,517,244 + eutJ BNILDI_12540 0.80 -1.3 2,517,266 + eutJ BNILDI_12540 0.83 -0.4 2,517,267 - eutJ BNILDI_12540 0.83 -0.1 2,517,325 + -3.3 2,517,368 + +0.0 2,517,394 + +1.3 2,517,394 + +1.5 2,517,394 + -3.4 2,517,395 - -0.4 2,517,395 - -0.1 2,517,395 - -0.1 2,517,647 + eutG BNILDI_12545 0.21 -0.3 2,518,057 + eutG BNILDI_12545 0.56 -0.1 2,518,058 - eutG BNILDI_12545 0.56 +0.4 2,518,058 - eutG BNILDI_12545 0.56 +0.4 2,518,075 - eutG BNILDI_12545 0.57 -1.6 2,518,148 - eutG BNILDI_12545 0.63 +1.0 2,518,195 - eutG BNILDI_12545 0.67 -2.7 2,518,195 - eutG BNILDI_12545 0.67 -1.8 2,518,273 - eutG BNILDI_12545 0.74 -0.3
Or see this region's nucleotide sequence