Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfA and bcp are separated by 252 nucleotides bcp and gcvR overlap by 1 nucleotides gcvR and dapA are separated by 145 nucleotides
BNILDI_12370: hyfA - hydrogenase 4 subunit HyfA, at 2,484,330 to 2,484,947
hyfA
BNILDI_12375: bcp - thioredoxin-dependent thiol peroxidase, at 2,485,200 to 2,485,670
bcp
BNILDI_12380: gcvR - glycine cleavage system transcriptional repressor, at 2,485,670 to 2,486,242
gcvR
BNILDI_12385: dapA - 4-hydroxy-tetrahydrodipicolinate synthase, at 2,486,388 to 2,487,266
dapA
Position (kb)
2485
2486
2487 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2484.856 kb on - strand, within hyfA at 2484.869 kb on + strand, within hyfA at 2484.870 kb on - strand, within hyfA at 2484.870 kb on - strand, within hyfA at 2484.899 kb on + strand at 2484.899 kb on + strand at 2484.899 kb on + strand at 2484.899 kb on + strand at 2484.902 kb on + strand at 2484.902 kb on + strand at 2484.902 kb on + strand at 2484.903 kb on - strand at 2484.903 kb on - strand at 2484.903 kb on - strand at 2484.903 kb on - strand at 2484.911 kb on + strand at 2484.912 kb on - strand at 2485.157 kb on - strand at 2485.157 kb on - strand at 2485.157 kb on - strand at 2485.197 kb on + strand at 2485.404 kb on - strand, within bcp at 2485.404 kb on - strand, within bcp at 2485.512 kb on + strand, within bcp at 2485.527 kb on + strand, within bcp at 2485.565 kb on - strand, within bcp at 2485.565 kb on - strand, within bcp at 2485.601 kb on + strand, within bcp at 2485.602 kb on - strand, within bcp at 2485.671 kb on + strand at 2485.675 kb on + strand at 2485.675 kb on + strand at 2485.676 kb on - strand at 2485.745 kb on - strand, within gcvR at 2485.748 kb on + strand, within gcvR at 2485.749 kb on - strand, within gcvR at 2485.882 kb on + strand, within gcvR at 2485.883 kb on - strand, within gcvR at 2485.883 kb on - strand, within gcvR at 2485.883 kb on - strand, within gcvR at 2485.883 kb on - strand, within gcvR at 2486.010 kb on - strand, within gcvR at 2486.059 kb on + strand, within gcvR at 2486.090 kb on - strand, within gcvR at 2486.093 kb on - strand, within gcvR at 2486.093 kb on - strand, within gcvR at 2486.093 kb on - strand, within gcvR at 2486.093 kb on - strand, within gcvR at 2486.137 kb on + strand, within gcvR at 2486.221 kb on - strand at 2486.301 kb on + strand at 2486.301 kb on + strand at 2486.301 kb on + strand at 2486.301 kb on + strand at 2486.301 kb on + strand at 2486.301 kb on + strand at 2486.301 kb on + strand at 2486.301 kb on + strand at 2486.302 kb on - strand at 2486.302 kb on - strand at 2486.302 kb on - strand at 2486.302 kb on - strand at 2486.302 kb on - strand at 2486.302 kb on - strand at 2486.302 kb on - strand at 2486.352 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,484,856 - hyfA BNILDI_12370 0.85 -0.4 2,484,869 + hyfA BNILDI_12370 0.87 -0.1 2,484,870 - hyfA BNILDI_12370 0.87 +0.7 2,484,870 - hyfA BNILDI_12370 0.87 -1.7 2,484,899 + -0.6 2,484,899 + -0.1 2,484,899 + +0.1 2,484,899 + +2.3 2,484,902 + +1.1 2,484,902 + +0.9 2,484,902 + +0.4 2,484,903 - -3.1 2,484,903 - -0.5 2,484,903 - +0.4 2,484,903 - -0.7 2,484,911 + +0.2 2,484,912 - -0.5 2,485,157 - +0.5 2,485,157 - +0.4 2,485,157 - -1.0 2,485,197 + +0.1 2,485,404 - bcp BNILDI_12375 0.43 -0.0 2,485,404 - bcp BNILDI_12375 0.43 -0.0 2,485,512 + bcp BNILDI_12375 0.66 -1.5 2,485,527 + bcp BNILDI_12375 0.69 -4.1 2,485,565 - bcp BNILDI_12375 0.77 -0.0 2,485,565 - bcp BNILDI_12375 0.77 -0.3 2,485,601 + bcp BNILDI_12375 0.85 +1.0 2,485,602 - bcp BNILDI_12375 0.85 +0.2 2,485,671 + -0.6 2,485,675 + +0.9 2,485,675 + +2.1 2,485,676 - +0.6 2,485,745 - gcvR BNILDI_12380 0.13 +0.4 2,485,748 + gcvR BNILDI_12380 0.14 -2.9 2,485,749 - gcvR BNILDI_12380 0.14 -0.0 2,485,882 + gcvR BNILDI_12380 0.37 -0.6 2,485,883 - gcvR BNILDI_12380 0.37 +0.3 2,485,883 - gcvR BNILDI_12380 0.37 -0.0 2,485,883 - gcvR BNILDI_12380 0.37 +0.2 2,485,883 - gcvR BNILDI_12380 0.37 +0.2 2,486,010 - gcvR BNILDI_12380 0.59 -1.1 2,486,059 + gcvR BNILDI_12380 0.68 +0.3 2,486,090 - gcvR BNILDI_12380 0.73 -0.4 2,486,093 - gcvR BNILDI_12380 0.74 -1.6 2,486,093 - gcvR BNILDI_12380 0.74 -0.7 2,486,093 - gcvR BNILDI_12380 0.74 +1.6 2,486,093 - gcvR BNILDI_12380 0.74 +0.5 2,486,137 + gcvR BNILDI_12380 0.82 -1.6 2,486,221 - +0.9 2,486,301 + -1.9 2,486,301 + -2.0 2,486,301 + -1.6 2,486,301 + +0.3 2,486,301 + -3.5 2,486,301 + +0.1 2,486,301 + -0.2 2,486,301 + -0.2 2,486,302 - +0.9 2,486,302 - +0.2 2,486,302 - -0.1 2,486,302 - +0.1 2,486,302 - +0.4 2,486,302 - -2.2 2,486,302 - +0.1 2,486,352 + -0.1
Or see this region's nucleotide sequence