Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepB and sseB are separated by 141 nucleotides sseB and sseA are separated by 492 nucleotides
BNILDI_12125: pepB - aminopeptidase PepB, at 2,429,051 to 2,430,334
pepB
BNILDI_12130: sseB - enhanced serine sensitivity protein SseB, at 2,430,476 to 2,431,252
sseB
BNILDI_12140: sseA - 3-mercaptopyruvate sulfurtransferase, at 2,431,745 to 2,432,590
sseA
Position (kb)
2430
2431
2432 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2429.520 kb on - strand, within pepB at 2429.520 kb on - strand, within pepB at 2429.521 kb on + strand, within pepB at 2429.582 kb on + strand, within pepB at 2429.583 kb on - strand, within pepB at 2429.615 kb on + strand, within pepB at 2429.616 kb on - strand, within pepB at 2429.629 kb on - strand, within pepB at 2429.633 kb on - strand, within pepB at 2429.844 kb on - strand, within pepB at 2430.337 kb on + strand at 2430.338 kb on - strand at 2430.341 kb on - strand at 2430.622 kb on + strand, within sseB at 2430.736 kb on - strand, within sseB at 2430.788 kb on - strand, within sseB at 2430.971 kb on - strand, within sseB at 2430.992 kb on - strand, within sseB at 2431.004 kb on - strand, within sseB at 2431.036 kb on + strand, within sseB at 2431.093 kb on - strand, within sseB at 2431.163 kb on - strand, within sseB at 2431.250 kb on + strand at 2431.251 kb on - strand at 2431.346 kb on + strand at 2431.350 kb on + strand at 2431.379 kb on + strand at 2431.379 kb on + strand at 2431.384 kb on - strand at 2431.401 kb on + strand at 2431.565 kb on + strand at 2431.565 kb on + strand at 2431.565 kb on + strand at 2431.566 kb on - strand at 2431.566 kb on - strand at 2431.582 kb on - strand at 2431.582 kb on - strand at 2431.585 kb on - strand at 2431.614 kb on + strand at 2431.625 kb on - strand at 2431.656 kb on - strand at 2431.663 kb on + strand at 2431.664 kb on - strand at 2431.746 kb on + strand at 2432.089 kb on + strand, within sseA at 2432.108 kb on + strand, within sseA at 2432.111 kb on + strand, within sseA at 2432.111 kb on + strand, within sseA at 2432.112 kb on - strand, within sseA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,429,520 - pepB BNILDI_12125 0.37 -0.8 2,429,520 - pepB BNILDI_12125 0.37 +0.2 2,429,521 + pepB BNILDI_12125 0.37 +0.3 2,429,582 + pepB BNILDI_12125 0.41 -1.4 2,429,583 - pepB BNILDI_12125 0.41 +0.5 2,429,615 + pepB BNILDI_12125 0.44 +0.5 2,429,616 - pepB BNILDI_12125 0.44 +0.3 2,429,629 - pepB BNILDI_12125 0.45 -1.8 2,429,633 - pepB BNILDI_12125 0.45 -0.1 2,429,844 - pepB BNILDI_12125 0.62 +1.5 2,430,337 + +0.9 2,430,338 - +0.4 2,430,341 - +1.1 2,430,622 + sseB BNILDI_12130 0.19 +0.5 2,430,736 - sseB BNILDI_12130 0.33 +0.8 2,430,788 - sseB BNILDI_12130 0.40 -1.0 2,430,971 - sseB BNILDI_12130 0.64 -1.7 2,430,992 - sseB BNILDI_12130 0.66 +0.9 2,431,004 - sseB BNILDI_12130 0.68 +1.2 2,431,036 + sseB BNILDI_12130 0.72 +0.4 2,431,093 - sseB BNILDI_12130 0.79 +0.1 2,431,163 - sseB BNILDI_12130 0.88 -2.8 2,431,250 + -2.0 2,431,251 - -2.1 2,431,346 + +2.0 2,431,350 + -1.7 2,431,379 + +0.3 2,431,379 + +0.1 2,431,384 - -2.2 2,431,401 + -0.0 2,431,565 + -0.8 2,431,565 + +0.2 2,431,565 + +2.6 2,431,566 - +0.5 2,431,566 - +0.4 2,431,582 - -0.4 2,431,582 - +0.9 2,431,585 - +0.2 2,431,614 + +1.2 2,431,625 - +1.0 2,431,656 - -1.0 2,431,663 + +0.3 2,431,664 - +1.2 2,431,746 + -4.2 2,432,089 + sseA BNILDI_12140 0.41 -3.0 2,432,108 + sseA BNILDI_12140 0.43 -1.0 2,432,111 + sseA BNILDI_12140 0.43 +1.2 2,432,111 + sseA BNILDI_12140 0.43 +1.0 2,432,112 - sseA BNILDI_12140 0.43 +0.1
Or see this region's nucleotide sequence