Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BNILDI_11505 and BNILDI_11510 are separated by 121 nucleotides BNILDI_11510 and BNILDI_11515 are separated by 32 nucleotides BNILDI_11515 and BNILDI_11520 are separated by 174 nucleotides BNILDI_11520 and BNILDI_11525 are separated by 22 nucleotides BNILDI_11525 and BNILDI_11530 are separated by 67 nucleotides
BNILDI_11505: BNILDI_11505 - phage repressor protein, at 2,316,872 to 2,317,504
_11505
BNILDI_11510: BNILDI_11510 - Rha family transcriptional regulator, at 2,317,626 to 2,317,868
_11510
BNILDI_11515: BNILDI_11515 - Protein ORFb in retron Ec67, at 2,317,901 to 2,318,410
_11515
BNILDI_11520: BNILDI_11520 - hypothetical protein, at 2,318,585 to 2,318,809
_11520
BNILDI_11525: BNILDI_11525 - Protein ORFc in retron Ec67, at 2,318,832 to 2,319,173
_11525
BNILDI_11530: BNILDI_11530 - DUF2732 domain-containing protein, at 2,319,241 to 2,319,474
_11530
Position (kb)
2317
2318
2319 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2317.594 kb on + strand at 2317.617 kb on + strand at 2317.626 kb on - strand at 2317.631 kb on + strand at 2317.638 kb on - strand at 2317.638 kb on - strand at 2317.804 kb on - strand, within BNILDI_11510 at 2317.962 kb on + strand, within BNILDI_11515 at 2318.106 kb on + strand, within BNILDI_11515 at 2318.107 kb on - strand, within BNILDI_11515 at 2318.107 kb on - strand, within BNILDI_11515 at 2318.183 kb on + strand, within BNILDI_11515 at 2318.245 kb on - strand, within BNILDI_11515 at 2318.272 kb on + strand, within BNILDI_11515 at 2318.284 kb on + strand, within BNILDI_11515 at 2318.284 kb on + strand, within BNILDI_11515 at 2318.285 kb on - strand, within BNILDI_11515 at 2318.285 kb on - strand, within BNILDI_11515 at 2318.418 kb on - strand at 2318.455 kb on + strand at 2318.478 kb on + strand at 2318.825 kb on - strand at 2318.841 kb on - strand at 2319.298 kb on - strand, within BNILDI_11530
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,317,594 + +0.3 2,317,617 + +0.5 2,317,626 - -0.4 2,317,631 + -0.3 2,317,638 - +0.7 2,317,638 - -0.6 2,317,804 - BNILDI_11510 0.73 -3.5 2,317,962 + BNILDI_11515 0.12 -0.6 2,318,106 + BNILDI_11515 0.40 +0.5 2,318,107 - BNILDI_11515 0.40 -0.9 2,318,107 - BNILDI_11515 0.40 +1.7 2,318,183 + BNILDI_11515 0.55 +1.1 2,318,245 - BNILDI_11515 0.67 +0.4 2,318,272 + BNILDI_11515 0.73 -0.5 2,318,284 + BNILDI_11515 0.75 -1.9 2,318,284 + BNILDI_11515 0.75 +1.0 2,318,285 - BNILDI_11515 0.75 +0.1 2,318,285 - BNILDI_11515 0.75 +0.1 2,318,418 - +0.5 2,318,455 + +0.4 2,318,478 + +1.3 2,318,825 - +0.6 2,318,841 - +1.7 2,319,298 - BNILDI_11530 0.24 +0.0
Or see this region's nucleotide sequence