Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt emrA and emrR are separated by 126 nucleotides emrR and ygaH are separated by 90 nucleotides ygaH and ygaZ overlap by 11 nucleotides ygaZ and ygaY are separated by 123 nucleotides
BNILDI_11180: emrA - multidrug efflux MFS transporter periplasmic adaptor subunit EmrA, at 2,255,101 to 2,256,273
emrA
BNILDI_11185: emrR - multidrug efflux transporter EmrAB transcriptional repressor EmrR, at 2,256,400 to 2,256,930
emrR
BNILDI_11190: ygaH - L-valine transporter subunit YgaH, at 2,257,021 to 2,257,356
ygaH
BNILDI_11195: ygaZ - L-valine exporter subunit YgaZ, at 2,257,346 to 2,258,083
ygaZ
BNILDI_11200: ygaY - Putative uncharacterized transporter YgaY, at 2,258,207 to 2,259,391
ygaY
Position (kb)
2257
2258 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2256.082 kb on - strand, within emrA at 2256.107 kb on + strand, within emrA at 2256.108 kb on - strand, within emrA at 2256.108 kb on - strand, within emrA at 2256.152 kb on + strand, within emrA at 2256.153 kb on - strand, within emrA at 2256.175 kb on - strand at 2256.181 kb on - strand at 2256.273 kb on + strand at 2256.273 kb on + strand at 2256.273 kb on + strand at 2256.274 kb on - strand at 2256.294 kb on + strand at 2256.369 kb on - strand at 2256.370 kb on + strand at 2256.370 kb on + strand at 2256.371 kb on - strand at 2256.371 kb on - strand at 2256.532 kb on - strand, within emrR at 2256.701 kb on - strand, within emrR at 2256.718 kb on - strand, within emrR at 2256.925 kb on - strand at 2256.992 kb on + strand at 2256.992 kb on + strand at 2256.993 kb on - strand at 2257.023 kb on - strand at 2257.023 kb on - strand at 2257.024 kb on + strand at 2257.024 kb on + strand at 2257.025 kb on - strand at 2257.025 kb on - strand at 2257.027 kb on - strand at 2257.027 kb on - strand at 2257.027 kb on - strand at 2257.027 kb on - strand at 2257.068 kb on + strand, within ygaH at 2257.069 kb on - strand, within ygaH at 2257.086 kb on + strand, within ygaH at 2257.086 kb on + strand, within ygaH at 2257.103 kb on + strand, within ygaH at 2257.169 kb on - strand, within ygaH at 2257.169 kb on - strand, within ygaH at 2257.307 kb on + strand, within ygaH at 2257.327 kb on + strand at 2257.339 kb on + strand at 2257.349 kb on + strand at 2257.349 kb on + strand at 2257.469 kb on + strand, within ygaZ at 2257.469 kb on + strand, within ygaZ at 2257.529 kb on - strand, within ygaZ at 2257.546 kb on + strand, within ygaZ at 2257.554 kb on - strand, within ygaZ at 2257.781 kb on - strand, within ygaZ at 2257.790 kb on - strand, within ygaZ at 2257.861 kb on + strand, within ygaZ at 2257.911 kb on + strand, within ygaZ at 2257.911 kb on + strand, within ygaZ at 2257.912 kb on - strand, within ygaZ at 2257.996 kb on + strand, within ygaZ at 2257.999 kb on - strand, within ygaZ at 2258.002 kb on + strand, within ygaZ at 2258.003 kb on - strand, within ygaZ at 2258.003 kb on - strand, within ygaZ at 2258.005 kb on + strand, within ygaZ at 2258.091 kb on - strand at 2258.112 kb on + strand at 2258.118 kb on + strand at 2258.173 kb on + strand at 2258.173 kb on + strand at 2258.173 kb on + strand at 2258.173 kb on + strand at 2258.174 kb on - strand at 2258.353 kb on + strand, within ygaY
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,256,082 - emrA BNILDI_11180 0.84 +0.1 2,256,107 + emrA BNILDI_11180 0.86 +0.1 2,256,108 - emrA BNILDI_11180 0.86 -4.2 2,256,108 - emrA BNILDI_11180 0.86 -1.5 2,256,152 + emrA BNILDI_11180 0.90 +0.2 2,256,153 - emrA BNILDI_11180 0.90 -0.4 2,256,175 - +0.0 2,256,181 - -0.4 2,256,273 + +1.8 2,256,273 + -0.7 2,256,273 + -2.4 2,256,274 - -0.6 2,256,294 + -0.2 2,256,369 - +0.8 2,256,370 + +0.7 2,256,370 + +1.3 2,256,371 - -0.5 2,256,371 - +0.6 2,256,532 - emrR BNILDI_11185 0.25 -0.1 2,256,701 - emrR BNILDI_11185 0.57 -0.0 2,256,718 - emrR BNILDI_11185 0.60 -0.0 2,256,925 - -0.7 2,256,992 + -0.1 2,256,992 + -0.8 2,256,993 - -0.0 2,257,023 - +0.4 2,257,023 - -1.0 2,257,024 + -0.1 2,257,024 + -1.8 2,257,025 - +0.2 2,257,025 - +1.4 2,257,027 - +0.6 2,257,027 - -0.3 2,257,027 - +1.4 2,257,027 - -2.7 2,257,068 + ygaH BNILDI_11190 0.14 +1.7 2,257,069 - ygaH BNILDI_11190 0.14 -0.2 2,257,086 + ygaH BNILDI_11190 0.19 -0.8 2,257,086 + ygaH BNILDI_11190 0.19 +1.3 2,257,103 + ygaH BNILDI_11190 0.24 -0.1 2,257,169 - ygaH BNILDI_11190 0.44 +0.3 2,257,169 - ygaH BNILDI_11190 0.44 +0.4 2,257,307 + ygaH BNILDI_11190 0.85 -0.3 2,257,327 + -3.3 2,257,339 + +0.5 2,257,349 + -0.5 2,257,349 + -2.8 2,257,469 + ygaZ BNILDI_11195 0.17 +0.1 2,257,469 + ygaZ BNILDI_11195 0.17 +0.4 2,257,529 - ygaZ BNILDI_11195 0.25 +0.5 2,257,546 + ygaZ BNILDI_11195 0.27 +0.9 2,257,554 - ygaZ BNILDI_11195 0.28 +0.1 2,257,781 - ygaZ BNILDI_11195 0.59 -1.6 2,257,790 - ygaZ BNILDI_11195 0.60 -0.9 2,257,861 + ygaZ BNILDI_11195 0.70 -0.8 2,257,911 + ygaZ BNILDI_11195 0.77 +1.8 2,257,911 + ygaZ BNILDI_11195 0.77 -1.7 2,257,912 - ygaZ BNILDI_11195 0.77 -0.0 2,257,996 + ygaZ BNILDI_11195 0.88 -2.3 2,257,999 - ygaZ BNILDI_11195 0.88 -2.2 2,258,002 + ygaZ BNILDI_11195 0.89 -1.5 2,258,003 - ygaZ BNILDI_11195 0.89 +0.9 2,258,003 - ygaZ BNILDI_11195 0.89 -0.3 2,258,005 + ygaZ BNILDI_11195 0.89 +0.4 2,258,091 - +0.3 2,258,112 + -0.0 2,258,118 + +0.4 2,258,173 + +1.4 2,258,173 + -1.4 2,258,173 + -0.7 2,258,173 + -0.5 2,258,174 - -3.8 2,258,353 + ygaY BNILDI_11200 0.12 +1.7
Or see this region's nucleotide sequence