Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gshA and luxS are separated by 149 nucleotides luxS and emrB are separated by 63 nucleotides emrB and emrA are separated by 16 nucleotides
BNILDI_11160: gshA - glutamate--cysteine ligase, at 2,251,261 to 2,252,817
gshA
BNILDI_11170: luxS - S-ribosylhomocysteine lyase, at 2,252,967 to 2,253,482
luxS
BNILDI_11175: emrB - multidrug efflux MFS transporter permease subunit EmrB, at 2,253,546 to 2,255,084
emrB
BNILDI_11180: emrA - multidrug efflux MFS transporter periplasmic adaptor subunit EmrA, at 2,255,101 to 2,256,273
emrA
Position (kb)
2253
2254
2255
2256 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2252.622 kb on + strand, within gshA at 2252.622 kb on + strand, within gshA at 2252.900 kb on + strand at 2252.901 kb on - strand at 2252.901 kb on - strand at 2252.901 kb on - strand at 2252.903 kb on - strand at 2252.903 kb on - strand at 2252.965 kb on - strand at 2252.977 kb on + strand at 2252.978 kb on - strand at 2252.978 kb on - strand at 2252.981 kb on + strand at 2252.982 kb on - strand at 2253.156 kb on - strand, within luxS at 2253.185 kb on - strand, within luxS at 2253.185 kb on - strand, within luxS at 2253.197 kb on + strand, within luxS at 2253.229 kb on - strand, within luxS at 2253.230 kb on + strand, within luxS at 2253.245 kb on + strand, within luxS at 2253.357 kb on + strand, within luxS at 2253.357 kb on + strand, within luxS at 2253.418 kb on + strand, within luxS at 2253.419 kb on - strand, within luxS at 2253.487 kb on + strand at 2253.493 kb on - strand at 2253.537 kb on + strand at 2253.537 kb on + strand at 2253.537 kb on + strand at 2253.538 kb on - strand at 2253.538 kb on - strand at 2253.538 kb on - strand at 2253.543 kb on + strand at 2253.543 kb on + strand at 2253.543 kb on + strand at 2253.543 kb on + strand at 2253.547 kb on + strand at 2253.547 kb on + strand at 2253.547 kb on + strand at 2253.547 kb on + strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.590 kb on + strand at 2253.745 kb on + strand, within emrB at 2253.746 kb on - strand, within emrB at 2253.746 kb on - strand, within emrB at 2253.947 kb on + strand, within emrB at 2254.025 kb on + strand, within emrB at 2254.028 kb on - strand, within emrB at 2254.054 kb on + strand, within emrB at 2254.054 kb on + strand, within emrB at 2254.054 kb on + strand, within emrB at 2254.055 kb on - strand, within emrB at 2254.058 kb on + strand, within emrB at 2254.058 kb on + strand, within emrB at 2254.058 kb on + strand, within emrB at 2254.058 kb on + strand, within emrB at 2254.058 kb on + strand, within emrB at 2254.059 kb on - strand, within emrB at 2254.059 kb on - strand, within emrB at 2254.059 kb on - strand, within emrB at 2254.061 kb on + strand, within emrB at 2254.061 kb on + strand, within emrB at 2254.062 kb on - strand, within emrB at 2254.140 kb on - strand, within emrB at 2254.241 kb on + strand, within emrB at 2254.241 kb on + strand, within emrB at 2254.242 kb on - strand, within emrB at 2254.242 kb on - strand, within emrB at 2254.242 kb on - strand, within emrB at 2254.242 kb on - strand, within emrB at 2254.243 kb on + strand, within emrB at 2254.244 kb on - strand, within emrB at 2254.244 kb on - strand, within emrB at 2254.244 kb on - strand, within emrB at 2254.256 kb on + strand, within emrB at 2254.352 kb on + strand, within emrB at 2254.606 kb on + strand, within emrB at 2254.693 kb on + strand, within emrB at 2254.697 kb on + strand, within emrB at 2254.697 kb on + strand, within emrB at 2254.707 kb on + strand, within emrB at 2254.707 kb on + strand, within emrB at 2254.707 kb on + strand, within emrB at 2254.985 kb on + strand at 2255.120 kb on - strand at 2255.197 kb on + strand at 2255.198 kb on - strand at 2255.203 kb on - strand at 2255.212 kb on + strand at 2255.420 kb on + strand, within emrA at 2255.455 kb on - strand, within emrA at 2255.520 kb on + strand, within emrA at 2255.521 kb on - strand, within emrA at 2255.620 kb on - strand, within emrA at 2255.622 kb on + strand, within emrA at 2255.653 kb on - strand, within emrA at 2255.656 kb on + strand, within emrA at 2255.732 kb on + strand, within emrA at 2255.732 kb on + strand, within emrA at 2255.732 kb on + strand, within emrA at 2255.733 kb on - strand, within emrA at 2255.846 kb on + strand, within emrA at 2255.846 kb on + strand, within emrA at 2256.082 kb on - strand, within emrA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,252,622 + gshA BNILDI_11160 0.87 -4.3 2,252,622 + gshA BNILDI_11160 0.87 +0.2 2,252,900 + +0.3 2,252,901 - -1.2 2,252,901 - +0.3 2,252,901 - +0.3 2,252,903 - -3.3 2,252,903 - -2.0 2,252,965 - +0.2 2,252,977 + -3.5 2,252,978 - -0.2 2,252,978 - +0.4 2,252,981 + -0.9 2,252,982 - +0.1 2,253,156 - luxS BNILDI_11170 0.37 -0.7 2,253,185 - luxS BNILDI_11170 0.42 +0.8 2,253,185 - luxS BNILDI_11170 0.42 -1.4 2,253,197 + luxS BNILDI_11170 0.45 +0.2 2,253,229 - luxS BNILDI_11170 0.51 -2.0 2,253,230 + luxS BNILDI_11170 0.51 -0.5 2,253,245 + luxS BNILDI_11170 0.54 -0.4 2,253,357 + luxS BNILDI_11170 0.76 +1.7 2,253,357 + luxS BNILDI_11170 0.76 +1.5 2,253,418 + luxS BNILDI_11170 0.87 -0.7 2,253,419 - luxS BNILDI_11170 0.88 +0.8 2,253,487 + +1.4 2,253,493 - +0.2 2,253,537 + +0.4 2,253,537 + +0.4 2,253,537 + +0.7 2,253,538 - -0.3 2,253,538 - +0.5 2,253,538 - -0.2 2,253,543 + +1.9 2,253,543 + -0.1 2,253,543 + +1.1 2,253,543 + -0.2 2,253,547 + -2.0 2,253,547 + +0.7 2,253,547 + -0.6 2,253,547 + +0.3 2,253,548 - +0.2 2,253,548 - -1.1 2,253,548 - +0.6 2,253,590 + +1.1 2,253,745 + emrB BNILDI_11175 0.13 -0.9 2,253,746 - emrB BNILDI_11175 0.13 +0.3 2,253,746 - emrB BNILDI_11175 0.13 -0.1 2,253,947 + emrB BNILDI_11175 0.26 +0.7 2,254,025 + emrB BNILDI_11175 0.31 +1.1 2,254,028 - emrB BNILDI_11175 0.31 -2.7 2,254,054 + emrB BNILDI_11175 0.33 +0.5 2,254,054 + emrB BNILDI_11175 0.33 +0.1 2,254,054 + emrB BNILDI_11175 0.33 +1.1 2,254,055 - emrB BNILDI_11175 0.33 -0.1 2,254,058 + emrB BNILDI_11175 0.33 +0.4 2,254,058 + emrB BNILDI_11175 0.33 -0.4 2,254,058 + emrB BNILDI_11175 0.33 +0.6 2,254,058 + emrB BNILDI_11175 0.33 +0.4 2,254,058 + emrB BNILDI_11175 0.33 -0.3 2,254,059 - emrB BNILDI_11175 0.33 -0.7 2,254,059 - emrB BNILDI_11175 0.33 -0.1 2,254,059 - emrB BNILDI_11175 0.33 -2.8 2,254,061 + emrB BNILDI_11175 0.33 +0.5 2,254,061 + emrB BNILDI_11175 0.33 -2.1 2,254,062 - emrB BNILDI_11175 0.34 +0.4 2,254,140 - emrB BNILDI_11175 0.39 +2.1 2,254,241 + emrB BNILDI_11175 0.45 +0.9 2,254,241 + emrB BNILDI_11175 0.45 +1.1 2,254,242 - emrB BNILDI_11175 0.45 +1.9 2,254,242 - emrB BNILDI_11175 0.45 -0.6 2,254,242 - emrB BNILDI_11175 0.45 -0.3 2,254,242 - emrB BNILDI_11175 0.45 -0.3 2,254,243 + emrB BNILDI_11175 0.45 -0.0 2,254,244 - emrB BNILDI_11175 0.45 -0.6 2,254,244 - emrB BNILDI_11175 0.45 +0.6 2,254,244 - emrB BNILDI_11175 0.45 +0.4 2,254,256 + emrB BNILDI_11175 0.46 -0.4 2,254,352 + emrB BNILDI_11175 0.52 +1.6 2,254,606 + emrB BNILDI_11175 0.69 -3.2 2,254,693 + emrB BNILDI_11175 0.75 +1.4 2,254,697 + emrB BNILDI_11175 0.75 +1.5 2,254,697 + emrB BNILDI_11175 0.75 +0.8 2,254,707 + emrB BNILDI_11175 0.75 +0.5 2,254,707 + emrB BNILDI_11175 0.75 -4.1 2,254,707 + emrB BNILDI_11175 0.75 -0.9 2,254,985 + -0.3 2,255,120 - -0.1 2,255,197 + -2.5 2,255,198 - -0.6 2,255,203 - -0.3 2,255,212 + +0.2 2,255,420 + emrA BNILDI_11180 0.27 -1.1 2,255,455 - emrA BNILDI_11180 0.30 +0.4 2,255,520 + emrA BNILDI_11180 0.36 -2.2 2,255,521 - emrA BNILDI_11180 0.36 +0.2 2,255,620 - emrA BNILDI_11180 0.44 +0.3 2,255,622 + emrA BNILDI_11180 0.44 -0.8 2,255,653 - emrA BNILDI_11180 0.47 -0.6 2,255,656 + emrA BNILDI_11180 0.47 -0.8 2,255,732 + emrA BNILDI_11180 0.54 +1.1 2,255,732 + emrA BNILDI_11180 0.54 -1.7 2,255,732 + emrA BNILDI_11180 0.54 -1.9 2,255,733 - emrA BNILDI_11180 0.54 -0.5 2,255,846 + emrA BNILDI_11180 0.64 +0.9 2,255,846 + emrA BNILDI_11180 0.64 +1.0 2,256,082 - emrA BNILDI_11180 0.84 +0.1
Or see this region's nucleotide sequence