Strain Fitness in Escherichia coli ECRC62 around BNILDI_10020

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygfZ and sdhE are separated by 242 nucleotidessdhE and ygfX overlap by 20 nucleotidesygfX and fldB are separated by 39 nucleotidesfldB and xerD are separated by 111 nucleotides BNILDI_10005: ygfZ - tRNA-modifying protein YgfZ, at 2,009,429 to 2,010,409 ygfZ BNILDI_10010: sdhE - FAD assembly factor SdhE, at 2,010,652 to 2,010,918 sdhE BNILDI_10015: ygfX - Inner membrane protein YgfX, at 2,010,899 to 2,011,306 ygfX BNILDI_10020: fldB - flavodoxin FldB, at 2,011,346 to 2,011,867 fldB BNILDI_10025: xerD - site-specific tyrosine recombinase XerD, at 2,011,979 to 2,012,875 xerD Position (kb) 2011 2012Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2010.413 kb on - strandat 2010.438 kb on - strandat 2010.438 kb on - strandat 2010.463 kb on - strandat 2010.463 kb on - strandat 2010.551 kb on + strandat 2010.575 kb on + strandat 2010.582 kb on - strandat 2010.611 kb on + strandat 2010.612 kb on - strandat 2010.714 kb on + strand, within sdhEat 2010.745 kb on + strand, within sdhEat 2010.982 kb on + strand, within ygfXat 2010.988 kb on - strand, within ygfXat 2010.988 kb on - strand, within ygfXat 2011.011 kb on - strand, within ygfXat 2011.076 kb on - strand, within ygfXat 2011.078 kb on + strand, within ygfXat 2011.078 kb on + strand, within ygfXat 2011.172 kb on - strand, within ygfXat 2011.172 kb on - strand, within ygfXat 2011.194 kb on + strand, within ygfXat 2011.194 kb on + strand, within ygfXat 2011.224 kb on + strand, within ygfXat 2011.224 kb on + strand, within ygfXat 2011.225 kb on - strand, within ygfXat 2011.323 kb on + strandat 2011.324 kb on - strandat 2011.353 kb on + strandat 2011.353 kb on + strandat 2011.399 kb on + strand, within fldBat 2011.400 kb on - strand, within fldBat 2011.427 kb on - strand, within fldBat 2011.731 kb on + strand, within fldBat 2011.732 kb on - strand, within fldBat 2011.742 kb on + strand, within fldBat 2011.772 kb on + strand, within fldBat 2011.772 kb on + strand, within fldBat 2011.772 kb on + strand, within fldBat 2011.772 kb on + strand, within fldBat 2011.772 kb on + strand, within fldBat 2011.773 kb on - strand, within fldBat 2011.796 kb on - strand, within fldBat 2011.831 kb on - strandat 2011.861 kb on + strandat 2011.905 kb on + strandat 2011.906 kb on - strandat 2011.910 kb on + strandat 2011.924 kb on + strandat 2011.946 kb on - strandat 2011.967 kb on + strandat 2012.050 kb on + strandat 2012.405 kb on + strand, within xerDat 2012.405 kb on + strand, within xerDat 2012.406 kb on - strand, within xerDat 2012.406 kb on - strand, within xerDat 2012.406 kb on - strand, within xerDat 2012.739 kb on + strand, within xerD

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,010,413 - -2.7
2,010,438 - -1.6
2,010,438 - -0.5
2,010,463 - -0.2
2,010,463 - +0.3
2,010,551 + +0.3
2,010,575 + -0.0
2,010,582 - -0.2
2,010,611 + +2.2
2,010,612 - -3.2
2,010,714 + sdhE BNILDI_10010 0.23 -1.8
2,010,745 + sdhE BNILDI_10010 0.35 -2.4
2,010,982 + ygfX BNILDI_10015 0.20 -0.4
2,010,988 - ygfX BNILDI_10015 0.22 -1.1
2,010,988 - ygfX BNILDI_10015 0.22 +0.6
2,011,011 - ygfX BNILDI_10015 0.27 -0.4
2,011,076 - ygfX BNILDI_10015 0.43 +1.0
2,011,078 + ygfX BNILDI_10015 0.44 -0.2
2,011,078 + ygfX BNILDI_10015 0.44 +0.2
2,011,172 - ygfX BNILDI_10015 0.67 -1.1
2,011,172 - ygfX BNILDI_10015 0.67 +1.8
2,011,194 + ygfX BNILDI_10015 0.72 +0.0
2,011,194 + ygfX BNILDI_10015 0.72 +0.1
2,011,224 + ygfX BNILDI_10015 0.80 -0.5
2,011,224 + ygfX BNILDI_10015 0.80 +1.0
2,011,225 - ygfX BNILDI_10015 0.80 +1.1
2,011,323 + -0.8
2,011,324 - -3.2
2,011,353 + -0.4
2,011,353 + +0.2
2,011,399 + fldB BNILDI_10020 0.10 -3.7
2,011,400 - fldB BNILDI_10020 0.10 -1.9
2,011,427 - fldB BNILDI_10020 0.16 -3.9
2,011,731 + fldB BNILDI_10020 0.74 +0.6
2,011,732 - fldB BNILDI_10020 0.74 -2.1
2,011,742 + fldB BNILDI_10020 0.76 -3.1
2,011,772 + fldB BNILDI_10020 0.82 -3.1
2,011,772 + fldB BNILDI_10020 0.82 -1.5
2,011,772 + fldB BNILDI_10020 0.82 -0.9
2,011,772 + fldB BNILDI_10020 0.82 -0.7
2,011,772 + fldB BNILDI_10020 0.82 -1.8
2,011,773 - fldB BNILDI_10020 0.82 +0.3
2,011,796 - fldB BNILDI_10020 0.86 -0.3
2,011,831 - -0.6
2,011,861 + -0.9
2,011,905 + -2.3
2,011,906 - +0.5
2,011,910 + -0.5
2,011,924 + +0.6
2,011,946 - +1.6
2,011,967 + -0.1
2,012,050 + -1.2
2,012,405 + xerD BNILDI_10025 0.47 -0.9
2,012,405 + xerD BNILDI_10025 0.47 +0.7
2,012,406 - xerD BNILDI_10025 0.48 -0.3
2,012,406 - xerD BNILDI_10025 0.48 -1.2
2,012,406 - xerD BNILDI_10025 0.48 +1.3
2,012,739 + xerD BNILDI_10025 0.85 -0.3

Or see this region's nucleotide sequence