Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygfZ and sdhE are separated by 242 nucleotides sdhE and ygfX overlap by 20 nucleotides ygfX and fldB are separated by 39 nucleotides fldB and xerD are separated by 111 nucleotides
BNILDI_10005: ygfZ - tRNA-modifying protein YgfZ, at 2,009,429 to 2,010,409
ygfZ
BNILDI_10010: sdhE - FAD assembly factor SdhE, at 2,010,652 to 2,010,918
sdhE
BNILDI_10015: ygfX - Inner membrane protein YgfX, at 2,010,899 to 2,011,306
ygfX
BNILDI_10020: fldB - flavodoxin FldB, at 2,011,346 to 2,011,867
fldB
BNILDI_10025: xerD - site-specific tyrosine recombinase XerD, at 2,011,979 to 2,012,875
xerD
Position (kb)
2011
2012 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2010.413 kb on - strand at 2010.438 kb on - strand at 2010.438 kb on - strand at 2010.463 kb on - strand at 2010.463 kb on - strand at 2010.551 kb on + strand at 2010.575 kb on + strand at 2010.582 kb on - strand at 2010.611 kb on + strand at 2010.612 kb on - strand at 2010.714 kb on + strand, within sdhE at 2010.745 kb on + strand, within sdhE at 2010.982 kb on + strand, within ygfX at 2010.988 kb on - strand, within ygfX at 2010.988 kb on - strand, within ygfX at 2011.011 kb on - strand, within ygfX at 2011.076 kb on - strand, within ygfX at 2011.078 kb on + strand, within ygfX at 2011.078 kb on + strand, within ygfX at 2011.172 kb on - strand, within ygfX at 2011.172 kb on - strand, within ygfX at 2011.194 kb on + strand, within ygfX at 2011.194 kb on + strand, within ygfX at 2011.224 kb on + strand, within ygfX at 2011.224 kb on + strand, within ygfX at 2011.225 kb on - strand, within ygfX at 2011.323 kb on + strand at 2011.324 kb on - strand at 2011.353 kb on + strand at 2011.353 kb on + strand at 2011.399 kb on + strand, within fldB at 2011.400 kb on - strand, within fldB at 2011.427 kb on - strand, within fldB at 2011.731 kb on + strand, within fldB at 2011.732 kb on - strand, within fldB at 2011.742 kb on + strand, within fldB at 2011.772 kb on + strand, within fldB at 2011.772 kb on + strand, within fldB at 2011.772 kb on + strand, within fldB at 2011.772 kb on + strand, within fldB at 2011.772 kb on + strand, within fldB at 2011.773 kb on - strand, within fldB at 2011.796 kb on - strand, within fldB at 2011.831 kb on - strand at 2011.861 kb on + strand at 2011.905 kb on + strand at 2011.906 kb on - strand at 2011.910 kb on + strand at 2011.924 kb on + strand at 2011.946 kb on - strand at 2011.967 kb on + strand at 2012.050 kb on + strand at 2012.405 kb on + strand, within xerD at 2012.405 kb on + strand, within xerD at 2012.406 kb on - strand, within xerD at 2012.406 kb on - strand, within xerD at 2012.406 kb on - strand, within xerD at 2012.739 kb on + strand, within xerD
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,010,413 - -2.7 2,010,438 - -1.6 2,010,438 - -0.5 2,010,463 - -0.2 2,010,463 - +0.3 2,010,551 + +0.3 2,010,575 + -0.0 2,010,582 - -0.2 2,010,611 + +2.2 2,010,612 - -3.2 2,010,714 + sdhE BNILDI_10010 0.23 -1.8 2,010,745 + sdhE BNILDI_10010 0.35 -2.4 2,010,982 + ygfX BNILDI_10015 0.20 -0.4 2,010,988 - ygfX BNILDI_10015 0.22 -1.1 2,010,988 - ygfX BNILDI_10015 0.22 +0.6 2,011,011 - ygfX BNILDI_10015 0.27 -0.4 2,011,076 - ygfX BNILDI_10015 0.43 +1.0 2,011,078 + ygfX BNILDI_10015 0.44 -0.2 2,011,078 + ygfX BNILDI_10015 0.44 +0.2 2,011,172 - ygfX BNILDI_10015 0.67 -1.1 2,011,172 - ygfX BNILDI_10015 0.67 +1.8 2,011,194 + ygfX BNILDI_10015 0.72 +0.0 2,011,194 + ygfX BNILDI_10015 0.72 +0.1 2,011,224 + ygfX BNILDI_10015 0.80 -0.5 2,011,224 + ygfX BNILDI_10015 0.80 +1.0 2,011,225 - ygfX BNILDI_10015 0.80 +1.1 2,011,323 + -0.8 2,011,324 - -3.2 2,011,353 + -0.4 2,011,353 + +0.2 2,011,399 + fldB BNILDI_10020 0.10 -3.7 2,011,400 - fldB BNILDI_10020 0.10 -1.9 2,011,427 - fldB BNILDI_10020 0.16 -3.9 2,011,731 + fldB BNILDI_10020 0.74 +0.6 2,011,732 - fldB BNILDI_10020 0.74 -2.1 2,011,742 + fldB BNILDI_10020 0.76 -3.1 2,011,772 + fldB BNILDI_10020 0.82 -3.1 2,011,772 + fldB BNILDI_10020 0.82 -1.5 2,011,772 + fldB BNILDI_10020 0.82 -0.9 2,011,772 + fldB BNILDI_10020 0.82 -0.7 2,011,772 + fldB BNILDI_10020 0.82 -1.8 2,011,773 - fldB BNILDI_10020 0.82 +0.3 2,011,796 - fldB BNILDI_10020 0.86 -0.3 2,011,831 - -0.6 2,011,861 + -0.9 2,011,905 + -2.3 2,011,906 - +0.5 2,011,910 + -0.5 2,011,924 + +0.6 2,011,946 - +1.6 2,011,967 + -0.1 2,012,050 + -1.2 2,012,405 + xerD BNILDI_10025 0.47 -0.9 2,012,405 + xerD BNILDI_10025 0.47 +0.7 2,012,406 - xerD BNILDI_10025 0.48 -0.3 2,012,406 - xerD BNILDI_10025 0.48 -1.2 2,012,406 - xerD BNILDI_10025 0.48 +1.3 2,012,739 + xerD BNILDI_10025 0.85 -0.3
Or see this region's nucleotide sequence