Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gcvP and ygfF are separated by 260 nucleotides ygfF and bglA are separated by 56 nucleotides
BNILDI_09980: gcvP - aminomethyl-transferring glycine dehydrogenase, at 2,002,806 to 2,005,679
gcvP
BNILDI_09985: ygfF - SDR family oxidoreductase, at 2,005,940 to 2,006,683
ygfF
BNILDI_09990: bglA - 6-phospho-beta-glucosidase BglA, at 2,006,740 to 2,008,173
bglA
Position (kb)
2005
2006
2007 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2005.017 kb on - strand, within gcvP at 2005.110 kb on + strand, within gcvP at 2005.111 kb on - strand, within gcvP at 2005.111 kb on - strand, within gcvP at 2005.111 kb on - strand, within gcvP at 2005.176 kb on + strand, within gcvP at 2005.176 kb on + strand, within gcvP at 2005.177 kb on - strand, within gcvP at 2005.177 kb on - strand, within gcvP at 2005.179 kb on - strand, within gcvP at 2005.179 kb on - strand, within gcvP at 2005.215 kb on + strand, within gcvP at 2005.216 kb on - strand, within gcvP at 2005.216 kb on - strand, within gcvP at 2005.250 kb on + strand, within gcvP at 2005.324 kb on - strand, within gcvP at 2005.487 kb on + strand at 2005.593 kb on + strand at 2005.672 kb on - strand at 2005.678 kb on - strand at 2005.694 kb on + strand at 2005.739 kb on + strand at 2005.739 kb on + strand at 2005.996 kb on - strand at 2006.033 kb on + strand, within ygfF at 2006.074 kb on - strand, within ygfF at 2006.074 kb on - strand, within ygfF at 2006.396 kb on + strand, within ygfF at 2006.396 kb on + strand, within ygfF at 2006.396 kb on + strand, within ygfF at 2006.396 kb on + strand, within ygfF at 2006.397 kb on - strand, within ygfF at 2006.397 kb on - strand, within ygfF at 2006.504 kb on - strand, within ygfF at 2006.504 kb on - strand, within ygfF at 2006.504 kb on - strand, within ygfF at 2006.504 kb on - strand, within ygfF at 2006.504 kb on - strand, within ygfF at 2006.622 kb on + strand at 2006.625 kb on + strand at 2006.625 kb on + strand at 2006.626 kb on - strand at 2006.626 kb on - strand at 2006.642 kb on + strand at 2006.642 kb on + strand at 2006.643 kb on - strand at 2006.681 kb on + strand at 2006.682 kb on - strand at 2006.716 kb on + strand at 2006.716 kb on + strand at 2006.717 kb on - strand at 2006.717 kb on - strand at 2006.717 kb on - strand at 2006.741 kb on + strand at 2006.784 kb on + strand at 2006.785 kb on - strand at 2006.797 kb on - strand at 2006.797 kb on - strand at 2006.836 kb on - strand at 2006.843 kb on + strand at 2006.843 kb on + strand at 2006.843 kb on + strand at 2006.844 kb on - strand at 2006.847 kb on + strand at 2006.848 kb on - strand at 2006.915 kb on + strand, within bglA at 2006.939 kb on + strand, within bglA at 2006.940 kb on - strand, within bglA at 2007.058 kb on - strand, within bglA at 2007.084 kb on - strand, within bglA at 2007.084 kb on - strand, within bglA at 2007.084 kb on - strand, within bglA at 2007.084 kb on - strand, within bglA at 2007.112 kb on + strand, within bglA at 2007.230 kb on + strand, within bglA at 2007.230 kb on + strand, within bglA at 2007.230 kb on + strand, within bglA at 2007.231 kb on - strand, within bglA at 2007.248 kb on + strand, within bglA at 2007.271 kb on + strand, within bglA at 2007.271 kb on + strand, within bglA at 2007.272 kb on - strand, within bglA at 2007.272 kb on - strand, within bglA at 2007.303 kb on + strand, within bglA at 2007.335 kb on + strand, within bglA at 2007.335 kb on + strand, within bglA at 2007.335 kb on + strand, within bglA at 2007.336 kb on - strand, within bglA at 2007.336 kb on - strand, within bglA at 2007.336 kb on - strand, within bglA at 2007.459 kb on + strand, within bglA at 2007.460 kb on - strand, within bglA at 2007.545 kb on + strand, within bglA at 2007.545 kb on + strand, within bglA at 2007.546 kb on - strand, within bglA at 2007.546 kb on - strand, within bglA at 2007.546 kb on - strand, within bglA at 2007.578 kb on + strand, within bglA at 2007.579 kb on - strand, within bglA at 2007.579 kb on - strand, within bglA at 2007.659 kb on + strand, within bglA at 2007.660 kb on - strand, within bglA at 2007.660 kb on - strand, within bglA at 2007.669 kb on + strand, within bglA at 2007.670 kb on - strand, within bglA at 2007.670 kb on - strand, within bglA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,005,017 - gcvP BNILDI_09980 0.77 -0.4 2,005,110 + gcvP BNILDI_09980 0.80 -0.4 2,005,111 - gcvP BNILDI_09980 0.80 +0.2 2,005,111 - gcvP BNILDI_09980 0.80 +1.7 2,005,111 - gcvP BNILDI_09980 0.80 +0.1 2,005,176 + gcvP BNILDI_09980 0.82 +0.5 2,005,176 + gcvP BNILDI_09980 0.82 -2.2 2,005,177 - gcvP BNILDI_09980 0.82 -3.8 2,005,177 - gcvP BNILDI_09980 0.82 -0.6 2,005,179 - gcvP BNILDI_09980 0.83 -1.1 2,005,179 - gcvP BNILDI_09980 0.83 -4.0 2,005,215 + gcvP BNILDI_09980 0.84 -0.0 2,005,216 - gcvP BNILDI_09980 0.84 +0.2 2,005,216 - gcvP BNILDI_09980 0.84 +0.8 2,005,250 + gcvP BNILDI_09980 0.85 -2.3 2,005,324 - gcvP BNILDI_09980 0.88 -0.9 2,005,487 + -0.1 2,005,593 + -2.1 2,005,672 - +0.2 2,005,678 - +0.3 2,005,694 + -0.1 2,005,739 + +0.8 2,005,739 + +0.0 2,005,996 - +1.0 2,006,033 + ygfF BNILDI_09985 0.12 +0.7 2,006,074 - ygfF BNILDI_09985 0.18 -0.1 2,006,074 - ygfF BNILDI_09985 0.18 +0.7 2,006,396 + ygfF BNILDI_09985 0.61 -3.9 2,006,396 + ygfF BNILDI_09985 0.61 +0.0 2,006,396 + ygfF BNILDI_09985 0.61 +0.4 2,006,396 + ygfF BNILDI_09985 0.61 +1.5 2,006,397 - ygfF BNILDI_09985 0.61 -3.7 2,006,397 - ygfF BNILDI_09985 0.61 -0.3 2,006,504 - ygfF BNILDI_09985 0.76 +0.4 2,006,504 - ygfF BNILDI_09985 0.76 -2.9 2,006,504 - ygfF BNILDI_09985 0.76 +0.4 2,006,504 - ygfF BNILDI_09985 0.76 -0.8 2,006,504 - ygfF BNILDI_09985 0.76 -3.1 2,006,622 + -0.8 2,006,625 + -0.4 2,006,625 + +1.9 2,006,626 - -0.2 2,006,626 - -0.2 2,006,642 + +0.3 2,006,642 + -0.1 2,006,643 - -1.0 2,006,681 + +0.3 2,006,682 - +0.0 2,006,716 + -0.3 2,006,716 + -0.5 2,006,717 - -3.3 2,006,717 - +0.4 2,006,717 - +0.1 2,006,741 + -0.0 2,006,784 + -3.6 2,006,785 - -0.6 2,006,797 - -0.5 2,006,797 - +0.9 2,006,836 - +1.4 2,006,843 + -0.3 2,006,843 + -0.6 2,006,843 + -0.9 2,006,844 - -0.4 2,006,847 + -0.2 2,006,848 - -2.8 2,006,915 + bglA BNILDI_09990 0.12 -3.4 2,006,939 + bglA BNILDI_09990 0.14 -0.5 2,006,940 - bglA BNILDI_09990 0.14 -1.1 2,007,058 - bglA BNILDI_09990 0.22 +0.0 2,007,084 - bglA BNILDI_09990 0.24 -0.8 2,007,084 - bglA BNILDI_09990 0.24 -0.5 2,007,084 - bglA BNILDI_09990 0.24 +0.7 2,007,084 - bglA BNILDI_09990 0.24 +0.4 2,007,112 + bglA BNILDI_09990 0.26 -0.1 2,007,230 + bglA BNILDI_09990 0.34 -1.1 2,007,230 + bglA BNILDI_09990 0.34 +1.4 2,007,230 + bglA BNILDI_09990 0.34 +0.4 2,007,231 - bglA BNILDI_09990 0.34 -0.3 2,007,248 + bglA BNILDI_09990 0.35 +1.2 2,007,271 + bglA BNILDI_09990 0.37 -0.6 2,007,271 + bglA BNILDI_09990 0.37 +0.8 2,007,272 - bglA BNILDI_09990 0.37 -0.2 2,007,272 - bglA BNILDI_09990 0.37 -1.6 2,007,303 + bglA BNILDI_09990 0.39 -3.4 2,007,335 + bglA BNILDI_09990 0.41 +0.2 2,007,335 + bglA BNILDI_09990 0.41 +0.4 2,007,335 + bglA BNILDI_09990 0.41 -1.1 2,007,336 - bglA BNILDI_09990 0.42 +0.2 2,007,336 - bglA BNILDI_09990 0.42 +0.3 2,007,336 - bglA BNILDI_09990 0.42 -0.0 2,007,459 + bglA BNILDI_09990 0.50 -3.0 2,007,460 - bglA BNILDI_09990 0.50 -0.8 2,007,545 + bglA BNILDI_09990 0.56 -0.0 2,007,545 + bglA BNILDI_09990 0.56 +0.5 2,007,546 - bglA BNILDI_09990 0.56 +1.5 2,007,546 - bglA BNILDI_09990 0.56 -0.5 2,007,546 - bglA BNILDI_09990 0.56 +0.3 2,007,578 + bglA BNILDI_09990 0.58 -1.2 2,007,579 - bglA BNILDI_09990 0.59 -3.2 2,007,579 - bglA BNILDI_09990 0.59 +0.6 2,007,659 + bglA BNILDI_09990 0.64 -0.8 2,007,660 - bglA BNILDI_09990 0.64 -0.2 2,007,660 - bglA BNILDI_09990 0.64 -1.0 2,007,669 + bglA BNILDI_09990 0.65 -1.8 2,007,670 - bglA BNILDI_09990 0.65 +1.7 2,007,670 - bglA BNILDI_09990 0.65 -2.5
Or see this region's nucleotide sequence