Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yqgE and gshB are separated by 36 nucleotides gshB and rsmE are separated by 12 nucleotides rsmE and endA are separated by 79 nucleotides endA and sprT are separated by 94 nucleotides
BNILDI_09720: yqgE - YqgE/AlgH family protein, at 1,954,101 to 1,954,736
yqgE
BNILDI_09725: gshB - glutathione synthase, at 1,954,773 to 1,955,723
gshB
BNILDI_09730: rsmE - 16S rRNA (uracil(1498)-N(3))-methyltransferase, at 1,955,736 to 1,956,467
rsmE
BNILDI_09735: endA - deoxyribonuclease I, at 1,956,547 to 1,957,254
endA
BNILDI_09740: sprT - SprT family zinc-dependent metalloprotease, at 1,957,349 to 1,957,804
sprT
Position (kb)
1955
1956
1957 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1954.809 kb on + strand at 1954.877 kb on + strand, within gshB at 1954.878 kb on - strand, within gshB at 1955.323 kb on - strand, within gshB at 1955.323 kb on - strand, within gshB at 1955.424 kb on - strand, within gshB at 1955.435 kb on - strand, within gshB at 1955.435 kb on - strand, within gshB at 1955.466 kb on - strand, within gshB at 1955.612 kb on + strand, within gshB at 1955.613 kb on - strand, within gshB at 1955.614 kb on + strand, within gshB at 1955.615 kb on - strand, within gshB at 1955.757 kb on + strand at 1955.764 kb on - strand at 1955.927 kb on + strand, within rsmE at 1956.230 kb on + strand, within rsmE at 1956.375 kb on + strand, within rsmE at 1956.448 kb on + strand at 1956.449 kb on - strand at 1956.467 kb on + strand at 1956.467 kb on + strand at 1956.467 kb on + strand at 1956.503 kb on - strand at 1956.543 kb on + strand at 1956.543 kb on + strand at 1956.544 kb on - strand at 1956.581 kb on + strand at 1956.581 kb on + strand at 1956.582 kb on - strand at 1956.651 kb on - strand, within endA at 1956.707 kb on + strand, within endA at 1956.722 kb on + strand, within endA at 1956.722 kb on + strand, within endA at 1956.722 kb on + strand, within endA at 1956.722 kb on + strand, within endA at 1956.722 kb on + strand, within endA at 1956.723 kb on - strand, within endA at 1956.723 kb on - strand, within endA at 1956.723 kb on - strand, within endA at 1956.723 kb on - strand, within endA at 1956.728 kb on + strand, within endA at 1956.728 kb on + strand, within endA at 1956.728 kb on + strand, within endA at 1956.729 kb on - strand, within endA at 1956.813 kb on - strand, within endA at 1956.842 kb on + strand, within endA at 1956.846 kb on + strand, within endA at 1956.847 kb on - strand, within endA at 1956.847 kb on - strand, within endA at 1956.895 kb on - strand, within endA at 1956.946 kb on - strand, within endA at 1957.016 kb on - strand, within endA at 1957.104 kb on + strand, within endA at 1957.105 kb on - strand, within endA at 1957.176 kb on + strand, within endA at 1957.182 kb on + strand, within endA at 1957.250 kb on + strand at 1957.250 kb on + strand at 1957.251 kb on - strand at 1957.251 kb on - strand at 1957.251 kb on - strand at 1957.251 kb on - strand at 1957.259 kb on + strand at 1957.260 kb on - strand at 1957.338 kb on - strand at 1957.390 kb on - strand at 1957.410 kb on - strand, within sprT
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,954,809 + -2.4 1,954,877 + gshB BNILDI_09725 0.11 +0.3 1,954,878 - gshB BNILDI_09725 0.11 -1.4 1,955,323 - gshB BNILDI_09725 0.58 -0.9 1,955,323 - gshB BNILDI_09725 0.58 -0.0 1,955,424 - gshB BNILDI_09725 0.68 -1.0 1,955,435 - gshB BNILDI_09725 0.70 -2.7 1,955,435 - gshB BNILDI_09725 0.70 +1.2 1,955,466 - gshB BNILDI_09725 0.73 -0.1 1,955,612 + gshB BNILDI_09725 0.88 -0.5 1,955,613 - gshB BNILDI_09725 0.88 -1.0 1,955,614 + gshB BNILDI_09725 0.88 -0.5 1,955,615 - gshB BNILDI_09725 0.89 +1.8 1,955,757 + +2.0 1,955,764 - -1.9 1,955,927 + rsmE BNILDI_09730 0.26 -1.8 1,956,230 + rsmE BNILDI_09730 0.67 -2.2 1,956,375 + rsmE BNILDI_09730 0.87 +0.2 1,956,448 + +0.3 1,956,449 - -1.2 1,956,467 + +0.6 1,956,467 + +1.0 1,956,467 + +0.1 1,956,503 - -1.7 1,956,543 + +0.4 1,956,543 + +2.9 1,956,544 - -0.0 1,956,581 + +0.8 1,956,581 + -1.0 1,956,582 - +0.8 1,956,651 - endA BNILDI_09735 0.15 -0.1 1,956,707 + endA BNILDI_09735 0.23 +0.6 1,956,722 + endA BNILDI_09735 0.25 -1.6 1,956,722 + endA BNILDI_09735 0.25 +1.1 1,956,722 + endA BNILDI_09735 0.25 +0.6 1,956,722 + endA BNILDI_09735 0.25 +0.1 1,956,722 + endA BNILDI_09735 0.25 +0.4 1,956,723 - endA BNILDI_09735 0.25 +0.7 1,956,723 - endA BNILDI_09735 0.25 -0.1 1,956,723 - endA BNILDI_09735 0.25 +1.3 1,956,723 - endA BNILDI_09735 0.25 -1.6 1,956,728 + endA BNILDI_09735 0.26 +0.6 1,956,728 + endA BNILDI_09735 0.26 -0.6 1,956,728 + endA BNILDI_09735 0.26 -0.6 1,956,729 - endA BNILDI_09735 0.26 +1.3 1,956,813 - endA BNILDI_09735 0.38 +0.1 1,956,842 + endA BNILDI_09735 0.42 -0.8 1,956,846 + endA BNILDI_09735 0.42 +0.2 1,956,847 - endA BNILDI_09735 0.42 +0.1 1,956,847 - endA BNILDI_09735 0.42 -1.0 1,956,895 - endA BNILDI_09735 0.49 -0.3 1,956,946 - endA BNILDI_09735 0.56 -1.8 1,957,016 - endA BNILDI_09735 0.66 +0.0 1,957,104 + endA BNILDI_09735 0.79 -2.4 1,957,105 - endA BNILDI_09735 0.79 +0.5 1,957,176 + endA BNILDI_09735 0.89 +0.9 1,957,182 + endA BNILDI_09735 0.90 +0.8 1,957,250 + +1.1 1,957,250 + -0.4 1,957,251 - -0.7 1,957,251 - -2.5 1,957,251 - +0.2 1,957,251 - -0.6 1,957,259 + +0.6 1,957,260 - -2.5 1,957,338 - +1.3 1,957,390 - +0.1 1,957,410 - sprT BNILDI_09740 0.13 -0.4
Or see this region's nucleotide sequence