Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yprA and hepA are separated by 223 nucleotides hepA and yeeA are separated by 2 nucleotides
BNILDI_09540: yprA - DEAD/DEAH box helicase, at 1,906,370 to 1,912,684
yprA
BNILDI_09545: hepA - helicase, at 1,912,908 to 1,915,766
hepA
BNILDI_09550: yeeA - Site-specific DNA-methyltransferase (adenine-specific), at 1,915,769 to 1,920,664
yeeA
Position (kb)
1912
1913
1914
1915
1916 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1911.951 kb on + strand, within yprA at 1911.989 kb on - strand, within yprA at 1911.989 kb on - strand, within yprA at 1912.026 kb on + strand, within yprA at 1912.027 kb on - strand, within yprA at 1912.189 kb on - strand at 1912.202 kb on + strand at 1912.214 kb on + strand at 1912.219 kb on + strand at 1912.220 kb on - strand at 1912.222 kb on + strand at 1912.245 kb on + strand at 1912.325 kb on + strand at 1912.325 kb on + strand at 1912.401 kb on + strand at 1912.402 kb on - strand at 1912.444 kb on + strand at 1912.445 kb on - strand at 1912.471 kb on + strand at 1912.472 kb on - strand at 1912.523 kb on + strand at 1912.524 kb on - strand at 1912.524 kb on - strand at 1912.547 kb on - strand at 1912.661 kb on - strand at 1912.667 kb on + strand at 1912.667 kb on + strand at 1912.667 kb on + strand at 1912.668 kb on - strand at 1912.668 kb on - strand at 1912.668 kb on - strand at 1912.668 kb on - strand at 1912.682 kb on + strand at 1912.924 kb on - strand at 1913.002 kb on - strand at 1913.022 kb on - strand at 1913.081 kb on - strand at 1913.151 kb on + strand at 1913.152 kb on - strand at 1913.152 kb on - strand at 1913.152 kb on - strand at 1913.154 kb on - strand at 1913.193 kb on - strand at 1913.534 kb on + strand, within hepA at 1913.535 kb on - strand, within hepA at 1913.604 kb on - strand, within hepA at 1913.604 kb on - strand, within hepA at 1913.604 kb on - strand, within hepA at 1913.660 kb on + strand, within hepA at 1913.693 kb on + strand, within hepA at 1913.702 kb on + strand, within hepA at 1913.703 kb on - strand, within hepA at 1913.729 kb on + strand, within hepA at 1913.730 kb on - strand, within hepA at 1913.797 kb on - strand, within hepA at 1913.803 kb on - strand, within hepA at 1913.892 kb on - strand, within hepA at 1913.937 kb on + strand, within hepA at 1913.938 kb on - strand, within hepA at 1914.108 kb on - strand, within hepA at 1914.110 kb on + strand, within hepA at 1914.371 kb on - strand, within hepA at 1914.385 kb on + strand, within hepA at 1914.385 kb on + strand, within hepA at 1914.439 kb on + strand, within hepA at 1914.439 kb on + strand, within hepA at 1914.439 kb on + strand, within hepA at 1914.440 kb on - strand, within hepA at 1914.494 kb on + strand, within hepA at 1914.515 kb on - strand, within hepA at 1914.527 kb on + strand, within hepA at 1914.527 kb on + strand, within hepA at 1914.528 kb on - strand, within hepA at 1914.700 kb on - strand, within hepA at 1914.700 kb on - strand, within hepA at 1914.700 kb on - strand, within hepA at 1914.826 kb on + strand, within hepA at 1914.827 kb on - strand, within hepA at 1914.827 kb on - strand, within hepA at 1914.900 kb on + strand, within hepA at 1914.900 kb on + strand, within hepA at 1914.922 kb on - strand, within hepA at 1914.945 kb on - strand, within hepA at 1914.945 kb on - strand, within hepA at 1914.952 kb on - strand, within hepA at 1914.971 kb on + strand, within hepA at 1914.971 kb on + strand, within hepA at 1914.972 kb on - strand, within hepA at 1914.972 kb on - strand, within hepA at 1915.057 kb on - strand, within hepA at 1915.061 kb on - strand, within hepA at 1915.231 kb on - strand, within hepA at 1915.681 kb on - strand at 1915.681 kb on - strand at 1915.681 kb on - strand at 1915.817 kb on + strand at 1915.929 kb on + strand at 1915.930 kb on - strand at 1916.047 kb on + strand at 1916.090 kb on - strand at 1916.119 kb on + strand at 1916.270 kb on - strand, within yeeA at 1916.505 kb on + strand, within yeeA at 1916.506 kb on - strand, within yeeA at 1916.510 kb on - strand, within yeeA at 1916.607 kb on - strand, within yeeA at 1916.654 kb on + strand, within yeeA at 1916.682 kb on - strand, within yeeA at 1916.735 kb on + strand, within yeeA at 1916.741 kb on + strand, within yeeA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,911,951 + yprA BNILDI_09540 0.88 +0.4 1,911,989 - yprA BNILDI_09540 0.89 -0.3 1,911,989 - yprA BNILDI_09540 0.89 +0.2 1,912,026 + yprA BNILDI_09540 0.90 -0.4 1,912,027 - yprA BNILDI_09540 0.90 -1.2 1,912,189 - +0.4 1,912,202 + +0.3 1,912,214 + -3.8 1,912,219 + +0.6 1,912,220 - +1.0 1,912,222 + +0.1 1,912,245 + -1.4 1,912,325 + +0.3 1,912,325 + +0.5 1,912,401 + -2.7 1,912,402 - -1.0 1,912,444 + +1.4 1,912,445 - -2.9 1,912,471 + -1.4 1,912,472 - +1.6 1,912,523 + -0.3 1,912,524 - +0.0 1,912,524 - +0.2 1,912,547 - -0.1 1,912,661 - -1.1 1,912,667 + -0.1 1,912,667 + -0.8 1,912,667 + +0.3 1,912,668 - +1.3 1,912,668 - -0.4 1,912,668 - -2.5 1,912,668 - +0.7 1,912,682 + +0.7 1,912,924 - +0.1 1,913,002 - +0.4 1,913,022 - -0.7 1,913,081 - -0.1 1,913,151 + -2.6 1,913,152 - +0.1 1,913,152 - -1.3 1,913,152 - +0.5 1,913,154 - -1.3 1,913,193 - +1.7 1,913,534 + hepA BNILDI_09545 0.22 -0.0 1,913,535 - hepA BNILDI_09545 0.22 +0.2 1,913,604 - hepA BNILDI_09545 0.24 -0.6 1,913,604 - hepA BNILDI_09545 0.24 +1.3 1,913,604 - hepA BNILDI_09545 0.24 -0.1 1,913,660 + hepA BNILDI_09545 0.26 -0.6 1,913,693 + hepA BNILDI_09545 0.27 -0.1 1,913,702 + hepA BNILDI_09545 0.28 +0.4 1,913,703 - hepA BNILDI_09545 0.28 -0.5 1,913,729 + hepA BNILDI_09545 0.29 -1.1 1,913,730 - hepA BNILDI_09545 0.29 +1.1 1,913,797 - hepA BNILDI_09545 0.31 -0.3 1,913,803 - hepA BNILDI_09545 0.31 +0.4 1,913,892 - hepA BNILDI_09545 0.34 +0.4 1,913,937 + hepA BNILDI_09545 0.36 -3.3 1,913,938 - hepA BNILDI_09545 0.36 -2.2 1,914,108 - hepA BNILDI_09545 0.42 -0.7 1,914,110 + hepA BNILDI_09545 0.42 -0.6 1,914,371 - hepA BNILDI_09545 0.51 -0.9 1,914,385 + hepA BNILDI_09545 0.52 -0.3 1,914,385 + hepA BNILDI_09545 0.52 +0.6 1,914,439 + hepA BNILDI_09545 0.54 +1.9 1,914,439 + hepA BNILDI_09545 0.54 -2.2 1,914,439 + hepA BNILDI_09545 0.54 +1.2 1,914,440 - hepA BNILDI_09545 0.54 +0.4 1,914,494 + hepA BNILDI_09545 0.55 -0.4 1,914,515 - hepA BNILDI_09545 0.56 +0.2 1,914,527 + hepA BNILDI_09545 0.57 -0.8 1,914,527 + hepA BNILDI_09545 0.57 +1.1 1,914,528 - hepA BNILDI_09545 0.57 +2.1 1,914,700 - hepA BNILDI_09545 0.63 -3.0 1,914,700 - hepA BNILDI_09545 0.63 +0.6 1,914,700 - hepA BNILDI_09545 0.63 -1.2 1,914,826 + hepA BNILDI_09545 0.67 -0.4 1,914,827 - hepA BNILDI_09545 0.67 -4.0 1,914,827 - hepA BNILDI_09545 0.67 +1.1 1,914,900 + hepA BNILDI_09545 0.70 +0.3 1,914,900 + hepA BNILDI_09545 0.70 +0.5 1,914,922 - hepA BNILDI_09545 0.70 +0.5 1,914,945 - hepA BNILDI_09545 0.71 -1.2 1,914,945 - hepA BNILDI_09545 0.71 -1.3 1,914,952 - hepA BNILDI_09545 0.71 -0.5 1,914,971 + hepA BNILDI_09545 0.72 +0.0 1,914,971 + hepA BNILDI_09545 0.72 -0.3 1,914,972 - hepA BNILDI_09545 0.72 +0.8 1,914,972 - hepA BNILDI_09545 0.72 +0.0 1,915,057 - hepA BNILDI_09545 0.75 +0.7 1,915,061 - hepA BNILDI_09545 0.75 -0.4 1,915,231 - hepA BNILDI_09545 0.81 +0.9 1,915,681 - +0.0 1,915,681 - +0.7 1,915,681 - +0.4 1,915,817 + -0.2 1,915,929 + -0.2 1,915,930 - +0.9 1,916,047 + -1.4 1,916,090 - +0.2 1,916,119 + -1.0 1,916,270 - yeeA BNILDI_09550 0.10 +1.7 1,916,505 + yeeA BNILDI_09550 0.15 +2.2 1,916,506 - yeeA BNILDI_09550 0.15 -0.3 1,916,510 - yeeA BNILDI_09550 0.15 -2.9 1,916,607 - yeeA BNILDI_09550 0.17 +1.0 1,916,654 + yeeA BNILDI_09550 0.18 +0.1 1,916,682 - yeeA BNILDI_09550 0.19 +0.4 1,916,735 + yeeA BNILDI_09550 0.20 +0.4 1,916,741 + yeeA BNILDI_09550 0.20 +0.4
Or see this region's nucleotide sequence